Third Harmonic Generation microscopy distinguishes malignant cell grade in human breast tissue biopsies

Abstract The ability to distinguish and grade malignant cells during surgical procedures in a fast, non-invasive and staining-free manner is of high importance in tumor management. To this extend, Third Harmonic Generation (THG), Second Harmonic Generation (SHG) and Fourier-Transform Infrared (FTIR)...

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Autores principales:	Evangelia Gavgiotaki, George Filippidis, Vassilis Tsafas, Savvas Bovasianos, George Kenanakis, Vasilios Georgoulias, Maria Tzardi, Sofia Agelaki, Irene Athanassakis
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7b58e9de714342a8b9cdb73e06acdf0a
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spelling	oai:doaj.org-article:7b58e9de714342a8b9cdb73e06acdf0a2021-12-02T15:39:40ZThird Harmonic Generation microscopy distinguishes malignant cell grade in human breast tissue biopsies10.1038/s41598-020-67857-y2045-2322https://doaj.org/article/7b58e9de714342a8b9cdb73e06acdf0a2020-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-67857-yhttps://doaj.org/toc/2045-2322Abstract The ability to distinguish and grade malignant cells during surgical procedures in a fast, non-invasive and staining-free manner is of high importance in tumor management. To this extend, Third Harmonic Generation (THG), Second Harmonic Generation (SHG) and Fourier-Transform Infrared (FTIR) spectroscopy were applied to discriminate malignant from healthy cells in human breast tissue biopsies. Indeed, integration of non-linear processes into a single, unified microscopy platform offered complementary structural information within individual cells at the submicron level. Using a single laser beam, label-free THG imaging techniques provided important morphological information as to the mean nuclear and cytoplasmic area, cell volume and tissue intensity, which upon quantification could not only distinguish cancerous from benign breast tissues but also define disease severity. Simultaneously, collagen fibers that could be detected by SHG imaging showed a well structured continuity in benign tumor tissues, which were gradually disoriented along with disease severity. Combination of THG imaging with FTIR spectroscopy could provide a clearer distinction among the different grades of breast cancer, since FTIR analysis showed increased lipid concentrations in malignant tissues. Thus, the use of non-linear optical microscopy can be considered as powerful and harmless tool for tumor cell diagnostics even during real time surgery procedures.Evangelia GavgiotakiGeorge FilippidisVassilis TsafasSavvas BovasianosGeorge KenanakisVasilios GeorgouliasMaria TzardiSofia AgelakiIrene AthanassakisNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-13 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Evangelia Gavgiotaki George Filippidis Vassilis Tsafas Savvas Bovasianos George Kenanakis Vasilios Georgoulias Maria Tzardi Sofia Agelaki Irene Athanassakis Third Harmonic Generation microscopy distinguishes malignant cell grade in human breast tissue biopsies
description	Abstract The ability to distinguish and grade malignant cells during surgical procedures in a fast, non-invasive and staining-free manner is of high importance in tumor management. To this extend, Third Harmonic Generation (THG), Second Harmonic Generation (SHG) and Fourier-Transform Infrared (FTIR) spectroscopy were applied to discriminate malignant from healthy cells in human breast tissue biopsies. Indeed, integration of non-linear processes into a single, unified microscopy platform offered complementary structural information within individual cells at the submicron level. Using a single laser beam, label-free THG imaging techniques provided important morphological information as to the mean nuclear and cytoplasmic area, cell volume and tissue intensity, which upon quantification could not only distinguish cancerous from benign breast tissues but also define disease severity. Simultaneously, collagen fibers that could be detected by SHG imaging showed a well structured continuity in benign tumor tissues, which were gradually disoriented along with disease severity. Combination of THG imaging with FTIR spectroscopy could provide a clearer distinction among the different grades of breast cancer, since FTIR analysis showed increased lipid concentrations in malignant tissues. Thus, the use of non-linear optical microscopy can be considered as powerful and harmless tool for tumor cell diagnostics even during real time surgery procedures.
format	article
author	Evangelia Gavgiotaki George Filippidis Vassilis Tsafas Savvas Bovasianos George Kenanakis Vasilios Georgoulias Maria Tzardi Sofia Agelaki Irene Athanassakis
author_facet	Evangelia Gavgiotaki George Filippidis Vassilis Tsafas Savvas Bovasianos George Kenanakis Vasilios Georgoulias Maria Tzardi Sofia Agelaki Irene Athanassakis
author_sort	Evangelia Gavgiotaki
title	Third Harmonic Generation microscopy distinguishes malignant cell grade in human breast tissue biopsies
title_short	Third Harmonic Generation microscopy distinguishes malignant cell grade in human breast tissue biopsies
title_full	Third Harmonic Generation microscopy distinguishes malignant cell grade in human breast tissue biopsies
title_fullStr	Third Harmonic Generation microscopy distinguishes malignant cell grade in human breast tissue biopsies
title_full_unstemmed	Third Harmonic Generation microscopy distinguishes malignant cell grade in human breast tissue biopsies
title_sort	third harmonic generation microscopy distinguishes malignant cell grade in human breast tissue biopsies
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/7b58e9de714342a8b9cdb73e06acdf0a
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Third Harmonic Generation microscopy distinguishes malignant cell grade in human breast tissue biopsies

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