THE ROLE OF GENETIC POLYMORPHISMS OF GENE IL-1β (-31 Т/С) AND IL-6 (-174 G/C) IN THE COURSE OF CHRONIC HEPATITIS B AND C IN CHILDREN
Abstract. Objectives: Chronic viral hepatitis В (CHB) and С (CHC) comprise an important issue of modern pediatrics. Immunogenetic disorders are among key factors of their pathogenesis. The aim of present research was to reveal interactions between polymorphisms of cytokine IL-1β and IL-6 genes and c...
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oai:doaj.org-article:7b59fec2400b4a1bbf85cbca3af07e452021-11-18T08:03:43ZTHE ROLE OF GENETIC POLYMORPHISMS OF GENE IL-1β (-31 Т/С) AND IL-6 (-174 G/C) IN THE COURSE OF CHRONIC HEPATITIS B AND C IN CHILDREN1563-06252313-741X10.15789/1563-0625-2013-6-535-542https://doaj.org/article/7b59fec2400b4a1bbf85cbca3af07e452014-07-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/672https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XAbstract. Objectives: Chronic viral hepatitis В (CHB) and С (CHC) comprise an important issue of modern pediatrics. Immunogenetic disorders are among key factors of their pathogenesis. The aim of present research was to reveal interactions between polymorphisms of cytokine IL-1β and IL-6 genes and clinical characteristics of the disease in children.The study included forty-eight children aged from 6 to 17 years (a median of 12 years old), including thirty patients with CHC, and eighteen – with CHB. Polymorphism of cytokine genes – interleukin 1β (IL-1β) – T31C, interleukin 6 (IL-6) – G174C was evaluated by means of allele-specific polymerase chain reaction (PCR); the amplification products were detected by horizontal electrophoresis (“Litekh”, Russia).The groups of CHB and CHC patients were characterized by increased prevalence of CC genotype of IL- 1β Т/С -31gene and СС genotype of IL-6 -174 G/C gene variants, as compared to control group [OR = 3.66 (95% CI – 1.2-11.0) and OR = 2.81 (95% CI – 1.33-5.87)]. The IL-6 -174 G/C polymorphism of is associated with higher IL-1β synthesis in GG genotype carriers, when compared to GC (p = 0.016) and СС genotypes (p = 0.030). In children with active CHB and CHC, polymorphisms of IL-1β -31 ТС and СС, as well as IL-6 -174 СC, were related to the higher cytolysis level, while the genotypes IL-1β -31 ТТ and IL-6 -174 GC and GG – with absence of cytolytic features(p = 0.022; p = 0.038).Presence of polymorphisms of IL-1β and IL-6 genes may sufficiently contribute to susceptibility to chronic hepatitis development and have an impact on extent of clinical and immunological presentations of the disease.S. V. RomanovaТ. А. VidmanovaЕ. А. ZhukovaЕ. V. ErmolinaN. I. TolkachevaI. V. MayanskayaI. А. TimchenkoSPb RAACIarticlechronic viral hepatitisinterleukinsgene polymorphismil-1βil-6childrenImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 15, Iss 6, Pp 535-542 (2014) |
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DOAJ |
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RU |
| topic |
chronic viral hepatitis interleukins gene polymorphism il-1β il-6 children Immunologic diseases. Allergy RC581-607 |
| spellingShingle |
chronic viral hepatitis interleukins gene polymorphism il-1β il-6 children Immunologic diseases. Allergy RC581-607 S. V. Romanova Т. А. Vidmanova Е. А. Zhukova Е. V. Ermolina N. I. Tolkacheva I. V. Mayanskaya I. А. Timchenko THE ROLE OF GENETIC POLYMORPHISMS OF GENE IL-1β (-31 Т/С) AND IL-6 (-174 G/C) IN THE COURSE OF CHRONIC HEPATITIS B AND C IN CHILDREN |
| description |
Abstract. Objectives: Chronic viral hepatitis В (CHB) and С (CHC) comprise an important issue of modern pediatrics. Immunogenetic disorders are among key factors of their pathogenesis. The aim of present research was to reveal interactions between polymorphisms of cytokine IL-1β and IL-6 genes and clinical characteristics of the disease in children.The study included forty-eight children aged from 6 to 17 years (a median of 12 years old), including thirty patients with CHC, and eighteen – with CHB. Polymorphism of cytokine genes – interleukin 1β (IL-1β) – T31C, interleukin 6 (IL-6) – G174C was evaluated by means of allele-specific polymerase chain reaction (PCR); the amplification products were detected by horizontal electrophoresis (“Litekh”, Russia).The groups of CHB and CHC patients were characterized by increased prevalence of CC genotype of IL- 1β Т/С -31gene and СС genotype of IL-6 -174 G/C gene variants, as compared to control group [OR = 3.66 (95% CI – 1.2-11.0) and OR = 2.81 (95% CI – 1.33-5.87)]. The IL-6 -174 G/C polymorphism of is associated with higher IL-1β synthesis in GG genotype carriers, when compared to GC (p = 0.016) and СС genotypes (p = 0.030). In children with active CHB and CHC, polymorphisms of IL-1β -31 ТС and СС, as well as IL-6 -174 СC, were related to the higher cytolysis level, while the genotypes IL-1β -31 ТТ and IL-6 -174 GC and GG – with absence of cytolytic features(p = 0.022; p = 0.038).Presence of polymorphisms of IL-1β and IL-6 genes may sufficiently contribute to susceptibility to chronic hepatitis development and have an impact on extent of clinical and immunological presentations of the disease. |
| format |
article |
| author |
S. V. Romanova Т. А. Vidmanova Е. А. Zhukova Е. V. Ermolina N. I. Tolkacheva I. V. Mayanskaya I. А. Timchenko |
| author_facet |
S. V. Romanova Т. А. Vidmanova Е. А. Zhukova Е. V. Ermolina N. I. Tolkacheva I. V. Mayanskaya I. А. Timchenko |
| author_sort |
S. V. Romanova |
| title |
THE ROLE OF GENETIC POLYMORPHISMS OF GENE IL-1β (-31 Т/С) AND IL-6 (-174 G/C) IN THE COURSE OF CHRONIC HEPATITIS B AND C IN CHILDREN |
| title_short |
THE ROLE OF GENETIC POLYMORPHISMS OF GENE IL-1β (-31 Т/С) AND IL-6 (-174 G/C) IN THE COURSE OF CHRONIC HEPATITIS B AND C IN CHILDREN |
| title_full |
THE ROLE OF GENETIC POLYMORPHISMS OF GENE IL-1β (-31 Т/С) AND IL-6 (-174 G/C) IN THE COURSE OF CHRONIC HEPATITIS B AND C IN CHILDREN |
| title_fullStr |
THE ROLE OF GENETIC POLYMORPHISMS OF GENE IL-1β (-31 Т/С) AND IL-6 (-174 G/C) IN THE COURSE OF CHRONIC HEPATITIS B AND C IN CHILDREN |
| title_full_unstemmed |
THE ROLE OF GENETIC POLYMORPHISMS OF GENE IL-1β (-31 Т/С) AND IL-6 (-174 G/C) IN THE COURSE OF CHRONIC HEPATITIS B AND C IN CHILDREN |
| title_sort |
role of genetic polymorphisms of gene il-1β (-31 т/с) and il-6 (-174 g/c) in the course of chronic hepatitis b and c in children |
| publisher |
SPb RAACI |
| publishDate |
2014 |
| url |
https://doaj.org/article/7b59fec2400b4a1bbf85cbca3af07e45 |
| work_keys_str_mv |
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| _version_ |
1718422429151264768 |