Engineered MATE multidrug transporters reveal two functionally distinct ion-coupling pathways in NorM from Vibrio cholerae

Raturi and Nair et al. describe functional ion pathways within the MATE transporter NorM from Vibrio cholerae (NorM-VC). By creating chimeric proteins and mutants of NorM-VC and NorM-PS, they dissect the localisation of ion-coupling events and roles of conserved catalytic carboxylates in H+ binding...

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Autores principales:	Sagar Raturi, Asha V. Nair, Keiko Shinoda, Himansha Singh, Boyan Bai, Satoshi Murakami, Hideaki Fujitani, Hendrik W. van Veen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/7b5a1295b8814806b230f1ab59fe38f8
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spelling	oai:doaj.org-article:7b5a1295b8814806b230f1ab59fe38f82021-12-02T17:02:05ZEngineered MATE multidrug transporters reveal two functionally distinct ion-coupling pathways in NorM from Vibrio cholerae10.1038/s42003-021-02081-62399-3642https://doaj.org/article/7b5a1295b8814806b230f1ab59fe38f82021-05-01T00:00:00Zhttps://doi.org/10.1038/s42003-021-02081-6https://doaj.org/toc/2399-3642Raturi and Nair et al. describe functional ion pathways within the MATE transporter NorM from Vibrio cholerae (NorM-VC). By creating chimeric proteins and mutants of NorM-VC and NorM-PS, they dissect the localisation of ion-coupling events and roles of conserved catalytic carboxylates in H+ binding and Na+ coordination, with findings useful to the field of transporters and drug resistance pumps.Sagar RaturiAsha V. NairKeiko ShinodaHimansha SinghBoyan BaiSatoshi MurakamiHideaki FujitaniHendrik W. van VeenNature PortfolioarticleBiology (General)QH301-705.5ENCommunications Biology, Vol 4, Iss 1, Pp 1-17 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 Sagar Raturi Asha V. Nair Keiko Shinoda Himansha Singh Boyan Bai Satoshi Murakami Hideaki Fujitani Hendrik W. van Veen Engineered MATE multidrug transporters reveal two functionally distinct ion-coupling pathways in NorM from Vibrio cholerae
description	Raturi and Nair et al. describe functional ion pathways within the MATE transporter NorM from Vibrio cholerae (NorM-VC). By creating chimeric proteins and mutants of NorM-VC and NorM-PS, they dissect the localisation of ion-coupling events and roles of conserved catalytic carboxylates in H+ binding and Na+ coordination, with findings useful to the field of transporters and drug resistance pumps.
format	article
author	Sagar Raturi Asha V. Nair Keiko Shinoda Himansha Singh Boyan Bai Satoshi Murakami Hideaki Fujitani Hendrik W. van Veen
author_facet	Sagar Raturi Asha V. Nair Keiko Shinoda Himansha Singh Boyan Bai Satoshi Murakami Hideaki Fujitani Hendrik W. van Veen
author_sort	Sagar Raturi
title	Engineered MATE multidrug transporters reveal two functionally distinct ion-coupling pathways in NorM from Vibrio cholerae
title_short	Engineered MATE multidrug transporters reveal two functionally distinct ion-coupling pathways in NorM from Vibrio cholerae
title_full	Engineered MATE multidrug transporters reveal two functionally distinct ion-coupling pathways in NorM from Vibrio cholerae
title_fullStr	Engineered MATE multidrug transporters reveal two functionally distinct ion-coupling pathways in NorM from Vibrio cholerae
title_full_unstemmed	Engineered MATE multidrug transporters reveal two functionally distinct ion-coupling pathways in NorM from Vibrio cholerae
title_sort	engineered mate multidrug transporters reveal two functionally distinct ion-coupling pathways in norm from vibrio cholerae
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/7b5a1295b8814806b230f1ab59fe38f8
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Engineered MATE multidrug transporters reveal two functionally distinct ion-coupling pathways in NorM from Vibrio cholerae

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