Characterisation and Molecular Analysis of an Unusual Chimeric Methicillin Resistant Staphylococcus Aureus Strain and its Bacteriophages
In the context of microarray-based epidemiological typing of the clonal organism Staphylococcus aureus/MRSA, a strain was identified that did not belong to known clonal complexes. The molecular analysis by microarray-based typing yielded signals suggesting that it was a mosaic or hybrid strain of tw...
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Frontiers Media S.A.
2021
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oai:doaj.org-article:7b6d661890e04bf2b50c68b8f6cf65112021-11-18T06:10:50ZCharacterisation and Molecular Analysis of an Unusual Chimeric Methicillin Resistant Staphylococcus Aureus Strain and its Bacteriophages1664-802110.3389/fgene.2021.723958https://doaj.org/article/7b6d661890e04bf2b50c68b8f6cf65112021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fgene.2021.723958/fullhttps://doaj.org/toc/1664-8021In the context of microarray-based epidemiological typing of the clonal organism Staphylococcus aureus/MRSA, a strain was identified that did not belong to known clonal complexes. The molecular analysis by microarray-based typing yielded signals suggesting that it was a mosaic or hybrid strain of two lineages. To verify this result, the isolate was sequenced with both, short-read Illumina and long-read Nanopore technologies and analysed in detail. This supported the hypothesis that the genome of this strain, ST6610-MRSA-IVg comprised of segments originating from two different clonal complexes (CC). While the backbone of the strain’s genome, i.e., roughly 2 megabases, belongs to CC8, a continuous insert of 894 kb (approx. 30% of the genome) originated from CC140. Beside core genomic markers in the normal succession and orientation, this insert also included the mecA gene, coding for PbP2a and causing methicillin resistance, localised on an SCCmec IVg element. This particular SCCmec type was also previously observed in CC140 MRSA from African countries. A second conspicuous observation was the presence of the trimethoprim resistance gene dfrG within on a prophage that occupied an attachment site normally used by Panton-Valentine Leucocidin phages. This observation could indicate a role of large-scale chromosomal recombination in the evolution of S. aureus as well as a role of phages in the dissemination of antibiotic resistance genes.Sindy Burgold-VoigtSindy Burgold-VoigtStefan MoneckeStefan MoneckeStefan MoneckeAlexandra SimbeckThomas HolzmannBärbel KieningerElisabeth M. Liebler-TenorioSascha D. BraunSascha D. BraunMaximilian CollatzMaximilian CollatzCelia DiezelCelia DiezelElke MüllerElke MüllerWulf Schneider-BrachertRalf EhrichtRalf EhrichtRalf EhrichtFrontiers Media S.A.articleStaphylococcus aureustrimethoprimMRSAbacteriophageschromosomal replacementGeneticsQH426-470ENFrontiers in Genetics, Vol 12 (2021) |
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Staphylococcus aureus trimethoprim MRSA bacteriophages chromosomal replacement Genetics QH426-470 |
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Staphylococcus aureus trimethoprim MRSA bacteriophages chromosomal replacement Genetics QH426-470 Sindy Burgold-Voigt Sindy Burgold-Voigt Stefan Monecke Stefan Monecke Stefan Monecke Alexandra Simbeck Thomas Holzmann Bärbel Kieninger Elisabeth M. Liebler-Tenorio Sascha D. Braun Sascha D. Braun Maximilian Collatz Maximilian Collatz Celia Diezel Celia Diezel Elke Müller Elke Müller Wulf Schneider-Brachert Ralf Ehricht Ralf Ehricht Ralf Ehricht Characterisation and Molecular Analysis of an Unusual Chimeric Methicillin Resistant Staphylococcus Aureus Strain and its Bacteriophages |
| description |
In the context of microarray-based epidemiological typing of the clonal organism Staphylococcus aureus/MRSA, a strain was identified that did not belong to known clonal complexes. The molecular analysis by microarray-based typing yielded signals suggesting that it was a mosaic or hybrid strain of two lineages. To verify this result, the isolate was sequenced with both, short-read Illumina and long-read Nanopore technologies and analysed in detail. This supported the hypothesis that the genome of this strain, ST6610-MRSA-IVg comprised of segments originating from two different clonal complexes (CC). While the backbone of the strain’s genome, i.e., roughly 2 megabases, belongs to CC8, a continuous insert of 894 kb (approx. 30% of the genome) originated from CC140. Beside core genomic markers in the normal succession and orientation, this insert also included the mecA gene, coding for PbP2a and causing methicillin resistance, localised on an SCCmec IVg element. This particular SCCmec type was also previously observed in CC140 MRSA from African countries. A second conspicuous observation was the presence of the trimethoprim resistance gene dfrG within on a prophage that occupied an attachment site normally used by Panton-Valentine Leucocidin phages. This observation could indicate a role of large-scale chromosomal recombination in the evolution of S. aureus as well as a role of phages in the dissemination of antibiotic resistance genes. |
| format |
article |
| author |
Sindy Burgold-Voigt Sindy Burgold-Voigt Stefan Monecke Stefan Monecke Stefan Monecke Alexandra Simbeck Thomas Holzmann Bärbel Kieninger Elisabeth M. Liebler-Tenorio Sascha D. Braun Sascha D. Braun Maximilian Collatz Maximilian Collatz Celia Diezel Celia Diezel Elke Müller Elke Müller Wulf Schneider-Brachert Ralf Ehricht Ralf Ehricht Ralf Ehricht |
| author_facet |
Sindy Burgold-Voigt Sindy Burgold-Voigt Stefan Monecke Stefan Monecke Stefan Monecke Alexandra Simbeck Thomas Holzmann Bärbel Kieninger Elisabeth M. Liebler-Tenorio Sascha D. Braun Sascha D. Braun Maximilian Collatz Maximilian Collatz Celia Diezel Celia Diezel Elke Müller Elke Müller Wulf Schneider-Brachert Ralf Ehricht Ralf Ehricht Ralf Ehricht |
| author_sort |
Sindy Burgold-Voigt |
| title |
Characterisation and Molecular Analysis of an Unusual Chimeric Methicillin Resistant Staphylococcus Aureus Strain and its Bacteriophages |
| title_short |
Characterisation and Molecular Analysis of an Unusual Chimeric Methicillin Resistant Staphylococcus Aureus Strain and its Bacteriophages |
| title_full |
Characterisation and Molecular Analysis of an Unusual Chimeric Methicillin Resistant Staphylococcus Aureus Strain and its Bacteriophages |
| title_fullStr |
Characterisation and Molecular Analysis of an Unusual Chimeric Methicillin Resistant Staphylococcus Aureus Strain and its Bacteriophages |
| title_full_unstemmed |
Characterisation and Molecular Analysis of an Unusual Chimeric Methicillin Resistant Staphylococcus Aureus Strain and its Bacteriophages |
| title_sort |
characterisation and molecular analysis of an unusual chimeric methicillin resistant staphylococcus aureus strain and its bacteriophages |
| publisher |
Frontiers Media S.A. |
| publishDate |
2021 |
| url |
https://doaj.org/article/7b6d661890e04bf2b50c68b8f6cf6511 |
| work_keys_str_mv |
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