The Uptake and Deconjugation of Androstenone Sulfate in the Adipose Tissue of the Boar

Boars express high testicular levels of sulfotransferase enzymes, and consequently, the boar taint causing compound androstenone predominantly circulates as a steroid sulfate. Androstenone sulfate is suspected to function as a steroid reservoir that can be deconjugated to provide a source of free an...

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Autores principales:	Christine Bone, E. James Squires
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	pig boar taint androstenone androstenone sulfate steroid uptake deconjugation Veterinary medicine SF600-1100 Zoology QL1-991
Acceso en línea:	https://doaj.org/article/7b6fb09d3d574df18ad933f6ae765001
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spelling	oai:doaj.org-article:7b6fb09d3d574df18ad933f6ae7650012021-11-25T16:17:28ZThe Uptake and Deconjugation of Androstenone Sulfate in the Adipose Tissue of the Boar10.3390/ani111131582076-2615https://doaj.org/article/7b6fb09d3d574df18ad933f6ae7650012021-11-01T00:00:00Zhttps://www.mdpi.com/2076-2615/11/11/3158https://doaj.org/toc/2076-2615Boars express high testicular levels of sulfotransferase enzymes, and consequently, the boar taint causing compound androstenone predominantly circulates as a steroid sulfate. Androstenone sulfate is suspected to function as a steroid reservoir that can be deconjugated to provide a source of free androstenone for accumulation. Therefore, the purpose of this study was to characterize the uptake and deconjugation of androstenone sulfate in the adipose tissue of the boar. Real-time PCR was used to quantify the expression of steroid sulfatase (STS) and several organic anion transporting polypeptides (OATPs) in the adipose tissue. Additionally, [<sup>3</sup>H]-androstenone sulfate was incubated with adipocytes or supernatant from homogenized fat to assess steroid uptake and conversion, respectively. A positive correlation existed between OATP-B expression and androstenone sulfate uptake (r = 0.86, <i>p</i> = 0.03), as well as between STS expression and androstenone sulfate conversion (r = 0.76, <i>p</i> < 0.001). Moreover, fat androstenone concentrations were positively correlated (r = 0.85, <i>p</i> < 0.001) with androstenone sulfate conversion and tended to increase with STS expression in early maturing boars. This suggests that androstenone sulfate uptake and deconjugation are mediated by OATP-B and STS, respectively, which may influence the development of boar taint in early maturing animals.Christine BoneE. James SquiresMDPI AGarticlepigboar taintandrostenoneandrostenone sulfatesteroid uptakedeconjugationVeterinary medicineSF600-1100ZoologyQL1-991ENAnimals, Vol 11, Iss 3158, p 3158 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	pig boar taint androstenone androstenone sulfate steroid uptake deconjugation Veterinary medicine SF600-1100 Zoology QL1-991
spellingShingle	pig boar taint androstenone androstenone sulfate steroid uptake deconjugation Veterinary medicine SF600-1100 Zoology QL1-991 Christine Bone E. James Squires The Uptake and Deconjugation of Androstenone Sulfate in the Adipose Tissue of the Boar
description	Boars express high testicular levels of sulfotransferase enzymes, and consequently, the boar taint causing compound androstenone predominantly circulates as a steroid sulfate. Androstenone sulfate is suspected to function as a steroid reservoir that can be deconjugated to provide a source of free androstenone for accumulation. Therefore, the purpose of this study was to characterize the uptake and deconjugation of androstenone sulfate in the adipose tissue of the boar. Real-time PCR was used to quantify the expression of steroid sulfatase (STS) and several organic anion transporting polypeptides (OATPs) in the adipose tissue. Additionally, [<sup>3</sup>H]-androstenone sulfate was incubated with adipocytes or supernatant from homogenized fat to assess steroid uptake and conversion, respectively. A positive correlation existed between OATP-B expression and androstenone sulfate uptake (r = 0.86, <i>p</i> = 0.03), as well as between STS expression and androstenone sulfate conversion (r = 0.76, <i>p</i> < 0.001). Moreover, fat androstenone concentrations were positively correlated (r = 0.85, <i>p</i> < 0.001) with androstenone sulfate conversion and tended to increase with STS expression in early maturing boars. This suggests that androstenone sulfate uptake and deconjugation are mediated by OATP-B and STS, respectively, which may influence the development of boar taint in early maturing animals.
format	article
author	Christine Bone E. James Squires
author_facet	Christine Bone E. James Squires
author_sort	Christine Bone
title	The Uptake and Deconjugation of Androstenone Sulfate in the Adipose Tissue of the Boar
title_short	The Uptake and Deconjugation of Androstenone Sulfate in the Adipose Tissue of the Boar
title_full	The Uptake and Deconjugation of Androstenone Sulfate in the Adipose Tissue of the Boar
title_fullStr	The Uptake and Deconjugation of Androstenone Sulfate in the Adipose Tissue of the Boar
title_full_unstemmed	The Uptake and Deconjugation of Androstenone Sulfate in the Adipose Tissue of the Boar
title_sort	uptake and deconjugation of androstenone sulfate in the adipose tissue of the boar
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/7b6fb09d3d574df18ad933f6ae765001
work_keys_str_mv	AT christinebone theuptakeanddeconjugationofandrostenonesulfateintheadiposetissueoftheboar AT ejamessquires theuptakeanddeconjugationofandrostenonesulfateintheadiposetissueoftheboar AT christinebone uptakeanddeconjugationofandrostenonesulfateintheadiposetissueoftheboar AT ejamessquires uptakeanddeconjugationofandrostenonesulfateintheadiposetissueoftheboar
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The Uptake and Deconjugation of Androstenone Sulfate in the Adipose Tissue of the Boar

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