Short Communication: Immunostimulatory effect of tempoyak (fermented durian) on inducing cytokine production (IL-6 and TNF-?) by RAW 264.7 cells

Susanto S, Sumarpo A, Parikesit AA, Putra ABNN, Ishida E, Tabuchi K, Sugahara T. 2018. Short Communication: Immunostimulatory effect of tempoyak (fermented durian) on inducing cytokine production (IL-6 and TNF-?) by RAW 264.7 cells. Biodiversitas 19: 318-322. Indonesia is known to be home to various...

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Autores principales:	SULVANIA SUSANTO, ANTON SUMARPO, ARLI ADITYA PARIKESIT, AGUS BUDIAWAN NARO PUTRA, ERI ISHIDA, KAHO TABUCH, TAKUYA SUGAHARA
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2018
Materias:	cytokine production il-6 immunostimulatory tempoyak tnf-ð›¼ Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/7b725678827642bab7ae57e36d2958b8
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spelling	oai:doaj.org-article:7b725678827642bab7ae57e36d2958b82021-11-16T14:01:05ZShort Communication: Immunostimulatory effect of tempoyak (fermented durian) on inducing cytokine production (IL-6 and TNF-?) by RAW 264.7 cells1412-033X2085-472210.13057/biodiv/d190143https://doaj.org/article/7b725678827642bab7ae57e36d2958b82018-01-01T00:00:00Zhttps://smujo.id/biodiv/article/view/2367https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Susanto S, Sumarpo A, Parikesit AA, Putra ABNN, Ishida E, Tabuchi K, Sugahara T. 2018. Short Communication: Immunostimulatory effect of tempoyak (fermented durian) on inducing cytokine production (IL-6 and TNF-?) by RAW 264.7 cells. Biodiversitas 19: 318-322. Indonesia is known to be home to various fermented foods with many-reported usage as potential sources of probiotics. Tempoyak (fermented durian) is among one of the Indonesian fermented foods that are rarely studied for its bioactivities. This study was conducted to evaluate the potential bioactivities of tempoyak, particularly the immunostimulatory aspects. Water extract of tempoyak was prepared by suspending the freeze-dried tempoyak sample in distilled water. Immunostimulatory activity of tempoyak water extract was evaluated using mouse macrophage cell line RAW 264.7. ELISA was used to screen cytokine productions (IL-6 and TNF-?) by RAW 264.7 cells following treatment with tempoyak water extract. In addition, real-time RT-PCR was also used to determine IL-6 and TNF-? mRNA expression. We showed that water extract of tempoyak exerts immunostimulatory effects towards RAW 264.7 cells. This was observed from the increased production of IL-6 and TNF-? in a dose-dependent manner. This was also supported by increased IL-6 and TNF-? mRNA expression. Our finding suggests that tempoyak has immunostimulatory effects towards murine macrophage cell line RAW 264.7. However, further studies are needed to identify the specific compounds responsible for inducing immunostimulatory effects.SULVANIA SUSANTOANTON SUMARPOARLI ADITYA PARIKESITAGUS BUDIAWAN NARO PUTRAERI ISHIDAKAHO TABUCHTAKUYA SUGAHARAMBI & UNS Soloarticlecytokine productionil-6immunostimulatorytempoyaktnf-ð›¼Biology (General)QH301-705.5ENBiodiversitas, Vol 19, Iss 1, Pp 318-322 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	cytokine production il-6 immunostimulatory tempoyak tnf-ð›¼ Biology (General) QH301-705.5
spellingShingle	cytokine production il-6 immunostimulatory tempoyak tnf-ð›¼ Biology (General) QH301-705.5 SULVANIA SUSANTO ANTON SUMARPO ARLI ADITYA PARIKESIT AGUS BUDIAWAN NARO PUTRA ERI ISHIDA KAHO TABUCH TAKUYA SUGAHARA Short Communication: Immunostimulatory effect of tempoyak (fermented durian) on inducing cytokine production (IL-6 and TNF-?) by RAW 264.7 cells
description	Susanto S, Sumarpo A, Parikesit AA, Putra ABNN, Ishida E, Tabuchi K, Sugahara T. 2018. Short Communication: Immunostimulatory effect of tempoyak (fermented durian) on inducing cytokine production (IL-6 and TNF-?) by RAW 264.7 cells. Biodiversitas 19: 318-322. Indonesia is known to be home to various fermented foods with many-reported usage as potential sources of probiotics. Tempoyak (fermented durian) is among one of the Indonesian fermented foods that are rarely studied for its bioactivities. This study was conducted to evaluate the potential bioactivities of tempoyak, particularly the immunostimulatory aspects. Water extract of tempoyak was prepared by suspending the freeze-dried tempoyak sample in distilled water. Immunostimulatory activity of tempoyak water extract was evaluated using mouse macrophage cell line RAW 264.7. ELISA was used to screen cytokine productions (IL-6 and TNF-?) by RAW 264.7 cells following treatment with tempoyak water extract. In addition, real-time RT-PCR was also used to determine IL-6 and TNF-? mRNA expression. We showed that water extract of tempoyak exerts immunostimulatory effects towards RAW 264.7 cells. This was observed from the increased production of IL-6 and TNF-? in a dose-dependent manner. This was also supported by increased IL-6 and TNF-? mRNA expression. Our finding suggests that tempoyak has immunostimulatory effects towards murine macrophage cell line RAW 264.7. However, further studies are needed to identify the specific compounds responsible for inducing immunostimulatory effects.
format	article
author	SULVANIA SUSANTO ANTON SUMARPO ARLI ADITYA PARIKESIT AGUS BUDIAWAN NARO PUTRA ERI ISHIDA KAHO TABUCH TAKUYA SUGAHARA
author_facet	SULVANIA SUSANTO ANTON SUMARPO ARLI ADITYA PARIKESIT AGUS BUDIAWAN NARO PUTRA ERI ISHIDA KAHO TABUCH TAKUYA SUGAHARA
author_sort	SULVANIA SUSANTO
title	Short Communication: Immunostimulatory effect of tempoyak (fermented durian) on inducing cytokine production (IL-6 and TNF-?) by RAW 264.7 cells
title_short	Short Communication: Immunostimulatory effect of tempoyak (fermented durian) on inducing cytokine production (IL-6 and TNF-?) by RAW 264.7 cells
title_full	Short Communication: Immunostimulatory effect of tempoyak (fermented durian) on inducing cytokine production (IL-6 and TNF-?) by RAW 264.7 cells
title_fullStr	Short Communication: Immunostimulatory effect of tempoyak (fermented durian) on inducing cytokine production (IL-6 and TNF-?) by RAW 264.7 cells
title_full_unstemmed	Short Communication: Immunostimulatory effect of tempoyak (fermented durian) on inducing cytokine production (IL-6 and TNF-?) by RAW 264.7 cells
title_sort	short communication: immunostimulatory effect of tempoyak (fermented durian) on inducing cytokine production (il-6 and tnf-?) by raw 264.7 cells
publisher	MBI & UNS Solo
publishDate	2018
url	https://doaj.org/article/7b725678827642bab7ae57e36d2958b8
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Short Communication: Immunostimulatory effect of tempoyak (fermented durian) on inducing cytokine production (IL-6 and TNF-?) by RAW 264.7 cells

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