Transcriptional analysis of Lactobacillus brevis to N-butanol and ferulic acid stress responses.

<h4>Background</h4>The presence of anti-microbial phenolic compounds, such as the model compound ferulic acid, in biomass hydrolysates pose significant challenges to the widespread use of biomass in conjunction with whole cell biocatalysis or fermentation. Currently, these inhibitory com...

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Autores principales:	James Winkler, Katy C Kao
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7b766bcf301540c390b3bdcc20b372df
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spelling	oai:doaj.org-article:7b766bcf301540c390b3bdcc20b372df2021-11-18T06:48:53ZTranscriptional analysis of Lactobacillus brevis to N-butanol and ferulic acid stress responses.1932-620310.1371/journal.pone.0021438https://doaj.org/article/7b766bcf301540c390b3bdcc20b372df2011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21829598/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>The presence of anti-microbial phenolic compounds, such as the model compound ferulic acid, in biomass hydrolysates pose significant challenges to the widespread use of biomass in conjunction with whole cell biocatalysis or fermentation. Currently, these inhibitory compounds must be removed through additional downstream processing or sufficiently diluted to create environments suitable for most industrially important microbial strains. Simultaneously, product toxicity must also be overcome to allow for efficient production of next generation biofuels such as n-butanol, isopropanol, and others from these low cost feedstocks.<h4>Methodology and principal findings</h4>This study explores the high ferulic acid and n-butanol tolerance in Lactobacillus brevis, a lactic acid bacterium often found in fermentation processes, by global transcriptional response analysis. The transcriptional profile of L. brevis reveals that the presence of ferulic acid triggers the expression of currently uncharacterized membrane proteins, possibly in an effort to counteract ferulic acid induced changes in membrane fluidity and ion leakage. In contrast to the ferulic acid stress response, n-butanol challenges to growing cultures primarily induce genes within the fatty acid synthesis pathway and reduced the proportion of 19:1 cyclopropane fatty acid within the L. brevis membrane. Both inhibitors also triggered generalized stress responses. Separate attempts to alter flux through the Escherichia coli fatty acid synthesis by overexpressing acetyl-CoA carboxylase subunits and deleting cyclopropane fatty acid synthase (cfa) both failed to improve n-butanol tolerance in E. coli, indicating that additional components of the stress response are required to confer n-butanol resistance.<h4>Conclusions</h4>Several promising routes for understanding both ferulic acid and n-butanol tolerance have been identified from L. brevis gene expression data. These insights may be used to guide further engineering of model industrial organisms to better tolerate both classes of inhibitors to enable facile production of biofuels from lignocellulosic biomass.James WinklerKaty C KaoPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 8, p e21438 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q James Winkler Katy C Kao Transcriptional analysis of Lactobacillus brevis to N-butanol and ferulic acid stress responses.
description	<h4>Background</h4>The presence of anti-microbial phenolic compounds, such as the model compound ferulic acid, in biomass hydrolysates pose significant challenges to the widespread use of biomass in conjunction with whole cell biocatalysis or fermentation. Currently, these inhibitory compounds must be removed through additional downstream processing or sufficiently diluted to create environments suitable for most industrially important microbial strains. Simultaneously, product toxicity must also be overcome to allow for efficient production of next generation biofuels such as n-butanol, isopropanol, and others from these low cost feedstocks.<h4>Methodology and principal findings</h4>This study explores the high ferulic acid and n-butanol tolerance in Lactobacillus brevis, a lactic acid bacterium often found in fermentation processes, by global transcriptional response analysis. The transcriptional profile of L. brevis reveals that the presence of ferulic acid triggers the expression of currently uncharacterized membrane proteins, possibly in an effort to counteract ferulic acid induced changes in membrane fluidity and ion leakage. In contrast to the ferulic acid stress response, n-butanol challenges to growing cultures primarily induce genes within the fatty acid synthesis pathway and reduced the proportion of 19:1 cyclopropane fatty acid within the L. brevis membrane. Both inhibitors also triggered generalized stress responses. Separate attempts to alter flux through the Escherichia coli fatty acid synthesis by overexpressing acetyl-CoA carboxylase subunits and deleting cyclopropane fatty acid synthase (cfa) both failed to improve n-butanol tolerance in E. coli, indicating that additional components of the stress response are required to confer n-butanol resistance.<h4>Conclusions</h4>Several promising routes for understanding both ferulic acid and n-butanol tolerance have been identified from L. brevis gene expression data. These insights may be used to guide further engineering of model industrial organisms to better tolerate both classes of inhibitors to enable facile production of biofuels from lignocellulosic biomass.
format	article
author	James Winkler Katy C Kao
author_facet	James Winkler Katy C Kao
author_sort	James Winkler
title	Transcriptional analysis of Lactobacillus brevis to N-butanol and ferulic acid stress responses.
title_short	Transcriptional analysis of Lactobacillus brevis to N-butanol and ferulic acid stress responses.
title_full	Transcriptional analysis of Lactobacillus brevis to N-butanol and ferulic acid stress responses.
title_fullStr	Transcriptional analysis of Lactobacillus brevis to N-butanol and ferulic acid stress responses.
title_full_unstemmed	Transcriptional analysis of Lactobacillus brevis to N-butanol and ferulic acid stress responses.
title_sort	transcriptional analysis of lactobacillus brevis to n-butanol and ferulic acid stress responses.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/7b766bcf301540c390b3bdcc20b372df
work_keys_str_mv	AT jameswinkler transcriptionalanalysisoflactobacillusbrevistonbutanolandferulicacidstressresponses AT katyckao transcriptionalanalysisoflactobacillusbrevistonbutanolandferulicacidstressresponses
_version_	1718424359317536768

Transcriptional analysis of Lactobacillus brevis to N-butanol and ferulic acid stress responses.

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