Repurposing tRNAs for nonsense suppression

Here, the authors report de novo design, optimization and characterization of tRNAs that decode UGA stop codons in E. coli. The structure of the ribosome in a complex with the designed tRNA bound to a UGA stop codon suggests that distinct A-site ligands (tRNAs versus release factors) induce distinct...

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Autores principales:	Suki Albers, Bertrand Beckert, Marco C. Matthies, Chandra Sekhar Mandava, Raphael Schuster, Carolin Seuring, Maria Riedner, Suparna Sanyal, Andrew E. Torda, Daniel N. Wilson, Zoya Ignatova
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/7b78cafe4a344dc8aed8e39af165c5be
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spelling	oai:doaj.org-article:7b78cafe4a344dc8aed8e39af165c5be2021-12-02T17:44:59ZRepurposing tRNAs for nonsense suppression10.1038/s41467-021-24076-x2041-1723https://doaj.org/article/7b78cafe4a344dc8aed8e39af165c5be2021-06-01T00:00:00Zhttps://doi.org/10.1038/s41467-021-24076-xhttps://doaj.org/toc/2041-1723Here, the authors report de novo design, optimization and characterization of tRNAs that decode UGA stop codons in E. coli. The structure of the ribosome in a complex with the designed tRNA bound to a UGA stop codon suggests that distinct A-site ligands (tRNAs versus release factors) induce distinct conformation of the stop codon within the mRNA in the decoding center.Suki AlbersBertrand BeckertMarco C. MatthiesChandra Sekhar MandavaRaphael SchusterCarolin SeuringMaria RiednerSuparna SanyalAndrew E. TordaDaniel N. WilsonZoya IgnatovaNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Suki Albers Bertrand Beckert Marco C. Matthies Chandra Sekhar Mandava Raphael Schuster Carolin Seuring Maria Riedner Suparna Sanyal Andrew E. Torda Daniel N. Wilson Zoya Ignatova Repurposing tRNAs for nonsense suppression
description	Here, the authors report de novo design, optimization and characterization of tRNAs that decode UGA stop codons in E. coli. The structure of the ribosome in a complex with the designed tRNA bound to a UGA stop codon suggests that distinct A-site ligands (tRNAs versus release factors) induce distinct conformation of the stop codon within the mRNA in the decoding center.
format	article
author	Suki Albers Bertrand Beckert Marco C. Matthies Chandra Sekhar Mandava Raphael Schuster Carolin Seuring Maria Riedner Suparna Sanyal Andrew E. Torda Daniel N. Wilson Zoya Ignatova
author_facet	Suki Albers Bertrand Beckert Marco C. Matthies Chandra Sekhar Mandava Raphael Schuster Carolin Seuring Maria Riedner Suparna Sanyal Andrew E. Torda Daniel N. Wilson Zoya Ignatova
author_sort	Suki Albers
title	Repurposing tRNAs for nonsense suppression
title_short	Repurposing tRNAs for nonsense suppression
title_full	Repurposing tRNAs for nonsense suppression
title_fullStr	Repurposing tRNAs for nonsense suppression
title_full_unstemmed	Repurposing tRNAs for nonsense suppression
title_sort	repurposing trnas for nonsense suppression
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/7b78cafe4a344dc8aed8e39af165c5be
work_keys_str_mv	AT sukialbers repurposingtrnasfornonsensesuppression AT bertrandbeckert repurposingtrnasfornonsensesuppression AT marcocmatthies repurposingtrnasfornonsensesuppression AT chandrasekharmandava repurposingtrnasfornonsensesuppression AT raphaelschuster repurposingtrnasfornonsensesuppression AT carolinseuring repurposingtrnasfornonsensesuppression AT mariariedner repurposingtrnasfornonsensesuppression AT suparnasanyal repurposingtrnasfornonsensesuppression AT andrewetorda repurposingtrnasfornonsensesuppression AT danielnwilson repurposingtrnasfornonsensesuppression AT zoyaignatova repurposingtrnasfornonsensesuppression
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Repurposing tRNAs for nonsense suppression

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