Expression analysis of inflammasome sensors and implication of NLRP12 inflammasome in prostate cancer

Abstract Inflammasomes are multi-proteins complex regulating inflammation-associated signaling. While inflammation plays a critical role in cancer cell growth, studies remain uncharacterized on the role of inflammasomes in prostate cancer. Using Gene Expression Omnibus (GEO) public datasets, we scre...

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Autores principales:	Dev Karan, Ossama Tawfik, Seema Dubey
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7b7f964462ea41359eaf0de215bfbd41
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spelling	oai:doaj.org-article:7b7f964462ea41359eaf0de215bfbd412021-12-02T11:40:44ZExpression analysis of inflammasome sensors and implication of NLRP12 inflammasome in prostate cancer10.1038/s41598-017-04286-42045-2322https://doaj.org/article/7b7f964462ea41359eaf0de215bfbd412017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-04286-4https://doaj.org/toc/2045-2322Abstract Inflammasomes are multi-proteins complex regulating inflammation-associated signaling. While inflammation plays a critical role in cancer cell growth, studies remain uncharacterized on the role of inflammasomes in prostate cancer. Using Gene Expression Omnibus (GEO) public datasets, we screened the expression profiles of inflammasome sensors NLRP3, NLRC4, NLRP6, NRLP12, and AIM2 in prostate tumor tissues, and verified their mRNA level in a panel of prostate cancer cell lines. The selected expression of NLRP3 and NLRP12 inflammasomes was validated, and the clinical association was evaluated in human prostate archival tumor tissues. We observed that the expression of inflammasome sensors was dysregulated at the mRNA level except for the NLRP12. The intensity of NLRP12 immunostaining was significantly higher in malignant prostate as compared to their adjacent benign tissues. In contrast, the NLRP3 immunostaining in prostate tissues was heterogeneous. The inflammasome complex proteins ASC (apoptosis-associated speck-like protein containing a CARD) and pro-caspase-1, as well as its downstream targets IL-1β and IL-18 were confined to aggressive prostate cancer cells. These data suggest an increased expression of NLRP12 in association with prostate cancer and support the role of NLRP12 inflammasome complex regulating inflammatory cytokines in understanding the role of inflammation in the prostate cancer.Dev KaranOssama TawfikSeema DubeyNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Dev Karan Ossama Tawfik Seema Dubey Expression analysis of inflammasome sensors and implication of NLRP12 inflammasome in prostate cancer
description	Abstract Inflammasomes are multi-proteins complex regulating inflammation-associated signaling. While inflammation plays a critical role in cancer cell growth, studies remain uncharacterized on the role of inflammasomes in prostate cancer. Using Gene Expression Omnibus (GEO) public datasets, we screened the expression profiles of inflammasome sensors NLRP3, NLRC4, NLRP6, NRLP12, and AIM2 in prostate tumor tissues, and verified their mRNA level in a panel of prostate cancer cell lines. The selected expression of NLRP3 and NLRP12 inflammasomes was validated, and the clinical association was evaluated in human prostate archival tumor tissues. We observed that the expression of inflammasome sensors was dysregulated at the mRNA level except for the NLRP12. The intensity of NLRP12 immunostaining was significantly higher in malignant prostate as compared to their adjacent benign tissues. In contrast, the NLRP3 immunostaining in prostate tissues was heterogeneous. The inflammasome complex proteins ASC (apoptosis-associated speck-like protein containing a CARD) and pro-caspase-1, as well as its downstream targets IL-1β and IL-18 were confined to aggressive prostate cancer cells. These data suggest an increased expression of NLRP12 in association with prostate cancer and support the role of NLRP12 inflammasome complex regulating inflammatory cytokines in understanding the role of inflammation in the prostate cancer.
format	article
author	Dev Karan Ossama Tawfik Seema Dubey
author_facet	Dev Karan Ossama Tawfik Seema Dubey
author_sort	Dev Karan
title	Expression analysis of inflammasome sensors and implication of NLRP12 inflammasome in prostate cancer
title_short	Expression analysis of inflammasome sensors and implication of NLRP12 inflammasome in prostate cancer
title_full	Expression analysis of inflammasome sensors and implication of NLRP12 inflammasome in prostate cancer
title_fullStr	Expression analysis of inflammasome sensors and implication of NLRP12 inflammasome in prostate cancer
title_full_unstemmed	Expression analysis of inflammasome sensors and implication of NLRP12 inflammasome in prostate cancer
title_sort	expression analysis of inflammasome sensors and implication of nlrp12 inflammasome in prostate cancer
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/7b7f964462ea41359eaf0de215bfbd41
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_version_	1718395590414434304

Expression analysis of inflammasome sensors and implication of NLRP12 inflammasome in prostate cancer

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