Anti-tumor effects of brucine immuno-nanoparticles on hepatocellular carcinoma

Jian-Min Qin1, Pei-Hao Yin1, Qi Li1, Zhong-Qiu Sa1, Xia Sheng1, Lin Yang1, Tao Huang1, Min Zhang1, Ke-Pan Gao2, Qing-Hua Chen2, Jing-Wei Ma3, He-Bai Shen31Department of General Surgery, Putuo Hospital, Shanghai University of Traditional Chinese Medicine, 2National Pharmaceutical Engineering Research...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Qin JM, Yin PH, Li Q, Sa ZQ, Sheng X, Yang L, Huang T, Zhang M, Gao KP, Chen QH, Ma JW, Shen HB
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2012
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/7b84a2aacfd84c3c908b61a3e34a82cb
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:7b84a2aacfd84c3c908b61a3e34a82cb
record_format	dspace
spelling	oai:doaj.org-article:7b84a2aacfd84c3c908b61a3e34a82cb2021-12-02T05:16:56ZAnti-tumor effects of brucine immuno-nanoparticles on hepatocellular carcinoma1176-91141178-2013https://doaj.org/article/7b84a2aacfd84c3c908b61a3e34a82cb2012-01-01T00:00:00Zhttp://www.dovepress.com/anti-tumor-effects-of-brucine-immuno-nanoparticles-on-hepatocellular-c-a9110https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Jian-Min Qin1, Pei-Hao Yin1, Qi Li1, Zhong-Qiu Sa1, Xia Sheng1, Lin Yang1, Tao Huang1, Min Zhang1, Ke-Pan Gao2, Qing-Hua Chen2, Jing-Wei Ma3, He-Bai Shen31Department of General Surgery, Putuo Hospital, Shanghai University of Traditional Chinese Medicine, 2National Pharmaceutical Engineering Research Center; Shanghai Institute of Pharmaceutical Industry, 3Department of Physical Chemistry, Shanghai Normal University, Shanghai, People&#39;s Republic of ChinaBackground: Hepatocellular carcinoma is difficult to diagnose early, and most patients are already in the late stages of the disease when they are admitted to hospital. The total 5-year survival rate is less than 5%. Recent studies have showed that brucine has a good anti-tumor effect, but high toxicity, poor water solubility, short half-life, narrow therapeutic window, and a toxic dose that is close to the therapeutic dose, which all limit its clinical application. This study evaluated the effects of brucine immuno-nanoparticles (BIN) on hepatocellular carcinoma.Materials and methods: Anionic polymerization, chemical modification technology, and phacoemulsification technology were used to prepare a carboxylated polyethylene glycol-polylactic acid copolymer carrier material. Chemical coupling technology was utilized to develop anti-human AFP McAb-polyethylene glycol-polylactic acid copolymer BIN. The size, shape, zeta potential, drug loading, encapsulation efficiency, and release of these immune-nanoparticles were studied in vitro. The targeting, and growth, invasion, and metastasis inhibitory effects of this treatment on liver cancer SMMC-7721 cells were tested.Results: BIN were of uniform size with an average particle size of 249 &plusmn; 77 nm and zeta potential of -18.7 &plusmn; 4.19 mV. The encapsulation efficiency was 76.0% &plusmn; 2.3% and the drug load was 5.6% &plusmn; 0.2%. Complete uptake and even distribution around the liver cancer cell membrane were observed.Conclusion: BIN had even size distribution, was stable, and had a slow-releasing effect. BIN targeted the cell membrane of the liver cancer cell SMMC-7721 and significantly inhibited the growth, adhesion, invasion, and metastasis of SMMC-7721 cells. As a novel drug carrier system, BIN are a potentially promising targeting treatment for liver cancer.Keywords: cancer targeting, hepatocellular carcinoma, nanoparticles, targeted drug delivery, anti-tumor effectQin JMYin PHLi QSa ZQSheng XYang LHuang TZhang MGao KPChen QHMa JWShen HBDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2012, Iss default, Pp 369-379 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Qin JM Yin PH Li Q Sa ZQ Sheng X Yang L Huang T Zhang M Gao KP Chen QH Ma JW Shen HB Anti-tumor effects of brucine immuno-nanoparticles on hepatocellular carcinoma
description	Jian-Min Qin1, Pei-Hao Yin1, Qi Li1, Zhong-Qiu Sa1, Xia Sheng1, Lin Yang1, Tao Huang1, Min Zhang1, Ke-Pan Gao2, Qing-Hua Chen2, Jing-Wei Ma3, He-Bai Shen31Department of General Surgery, Putuo Hospital, Shanghai University of Traditional Chinese Medicine, 2National Pharmaceutical Engineering Research Center; Shanghai Institute of Pharmaceutical Industry, 3Department of Physical Chemistry, Shanghai Normal University, Shanghai, People&#39;s Republic of ChinaBackground: Hepatocellular carcinoma is difficult to diagnose early, and most patients are already in the late stages of the disease when they are admitted to hospital. The total 5-year survival rate is less than 5%. Recent studies have showed that brucine has a good anti-tumor effect, but high toxicity, poor water solubility, short half-life, narrow therapeutic window, and a toxic dose that is close to the therapeutic dose, which all limit its clinical application. This study evaluated the effects of brucine immuno-nanoparticles (BIN) on hepatocellular carcinoma.Materials and methods: Anionic polymerization, chemical modification technology, and phacoemulsification technology were used to prepare a carboxylated polyethylene glycol-polylactic acid copolymer carrier material. Chemical coupling technology was utilized to develop anti-human AFP McAb-polyethylene glycol-polylactic acid copolymer BIN. The size, shape, zeta potential, drug loading, encapsulation efficiency, and release of these immune-nanoparticles were studied in vitro. The targeting, and growth, invasion, and metastasis inhibitory effects of this treatment on liver cancer SMMC-7721 cells were tested.Results: BIN were of uniform size with an average particle size of 249 &plusmn; 77 nm and zeta potential of -18.7 &plusmn; 4.19 mV. The encapsulation efficiency was 76.0% &plusmn; 2.3% and the drug load was 5.6% &plusmn; 0.2%. Complete uptake and even distribution around the liver cancer cell membrane were observed.Conclusion: BIN had even size distribution, was stable, and had a slow-releasing effect. BIN targeted the cell membrane of the liver cancer cell SMMC-7721 and significantly inhibited the growth, adhesion, invasion, and metastasis of SMMC-7721 cells. As a novel drug carrier system, BIN are a potentially promising targeting treatment for liver cancer.Keywords: cancer targeting, hepatocellular carcinoma, nanoparticles, targeted drug delivery, anti-tumor effect
format	article
author	Qin JM Yin PH Li Q Sa ZQ Sheng X Yang L Huang T Zhang M Gao KP Chen QH Ma JW Shen HB
author_facet	Qin JM Yin PH Li Q Sa ZQ Sheng X Yang L Huang T Zhang M Gao KP Chen QH Ma JW Shen HB
author_sort	Qin JM
title	Anti-tumor effects of brucine immuno-nanoparticles on hepatocellular carcinoma
title_short	Anti-tumor effects of brucine immuno-nanoparticles on hepatocellular carcinoma
title_full	Anti-tumor effects of brucine immuno-nanoparticles on hepatocellular carcinoma
title_fullStr	Anti-tumor effects of brucine immuno-nanoparticles on hepatocellular carcinoma
title_full_unstemmed	Anti-tumor effects of brucine immuno-nanoparticles on hepatocellular carcinoma
title_sort	anti-tumor effects of brucine immuno-nanoparticles on hepatocellular carcinoma
publisher	Dove Medical Press
publishDate	2012
url	https://doaj.org/article/7b84a2aacfd84c3c908b61a3e34a82cb
work_keys_str_mv	AT qinjm antitumoreffectsofbrucineimmunonanoparticlesonhepatocellularcarcinoma AT yinph antitumoreffectsofbrucineimmunonanoparticlesonhepatocellularcarcinoma AT liq antitumoreffectsofbrucineimmunonanoparticlesonhepatocellularcarcinoma AT sazq antitumoreffectsofbrucineimmunonanoparticlesonhepatocellularcarcinoma AT shengx antitumoreffectsofbrucineimmunonanoparticlesonhepatocellularcarcinoma AT yangl antitumoreffectsofbrucineimmunonanoparticlesonhepatocellularcarcinoma AT huangt antitumoreffectsofbrucineimmunonanoparticlesonhepatocellularcarcinoma AT zhangm antitumoreffectsofbrucineimmunonanoparticlesonhepatocellularcarcinoma AT gaokp antitumoreffectsofbrucineimmunonanoparticlesonhepatocellularcarcinoma AT chenqh antitumoreffectsofbrucineimmunonanoparticlesonhepatocellularcarcinoma AT majw antitumoreffectsofbrucineimmunonanoparticlesonhepatocellularcarcinoma AT shenhb antitumoreffectsofbrucineimmunonanoparticlesonhepatocellularcarcinoma
_version_	1718400480177029120

Anti-tumor effects of brucine immuno-nanoparticles on hepatocellular carcinoma

Ejemplares similares