Methylated (−)-epigallocatechin 3-O-gallate potentiates the effect of split vaccine accompanied with upregulation of Toll-like receptor 5

Abstract Split-virus vaccine serves as a major countermeasure against influenza virus, but its effectiveness and protective action are not complete. We previously demonstrated the effect of Benifuuki, a green tea cultivar in Japan, on enhancing the split-virus vaccine–elicited immune response. Howev...

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Autores principales:	Motofumi Kumazoe, Kanako Takamatsu, Fuyumi Horie, Ren Yoshitomi, Hiroki Hamagami, Hiroshi Tanaka, Yoshinori Fujimura, Hirofumi Tachibana
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7b8788c280364e29bde46c56c5696202
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spelling	oai:doaj.org-article:7b8788c280364e29bde46c56c56962022021-12-05T12:15:57ZMethylated (−)-epigallocatechin 3-O-gallate potentiates the effect of split vaccine accompanied with upregulation of Toll-like receptor 510.1038/s41598-021-02346-42045-2322https://doaj.org/article/7b8788c280364e29bde46c56c56962022021-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-02346-4https://doaj.org/toc/2045-2322Abstract Split-virus vaccine serves as a major countermeasure against influenza virus, but its effectiveness and protective action are not complete. We previously demonstrated the effect of Benifuuki, a green tea cultivar in Japan, on enhancing the split-virus vaccine–elicited immune response. However, little is known about the detail mechanisms. Here, we show that EGCG3”Me intake significantly potentiated the vaccine-elicited hemagglutination inhibition titer increase. Flow cytometry analysis revealed the increased Toll-like receptor 5 (TLR5) expression after EGCG3”Me treatment in lamina propria dendritic cells (LPDCs) and macrophages, which play crucial roles in the humoral immune system. TLR5 expression correlated with the level of interleukin-6 (IL-6)/C–C chemokine type receptor 5, which are important mediators of the humoral immunity. Taken together, In vivo and ex vivo studies showed that EGCG3”Me potentiated the split-virus vaccine–elicited immune response accompanied with the upregulation of TLR5 in intestine and splenocyte macrophages.Motofumi KumazoeKanako TakamatsuFuyumi HorieRen YoshitomiHiroki HamagamiHiroshi TanakaYoshinori FujimuraHirofumi TachibanaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Motofumi Kumazoe Kanako Takamatsu Fuyumi Horie Ren Yoshitomi Hiroki Hamagami Hiroshi Tanaka Yoshinori Fujimura Hirofumi Tachibana Methylated (−)-epigallocatechin 3-O-gallate potentiates the effect of split vaccine accompanied with upregulation of Toll-like receptor 5
description	Abstract Split-virus vaccine serves as a major countermeasure against influenza virus, but its effectiveness and protective action are not complete. We previously demonstrated the effect of Benifuuki, a green tea cultivar in Japan, on enhancing the split-virus vaccine–elicited immune response. However, little is known about the detail mechanisms. Here, we show that EGCG3”Me intake significantly potentiated the vaccine-elicited hemagglutination inhibition titer increase. Flow cytometry analysis revealed the increased Toll-like receptor 5 (TLR5) expression after EGCG3”Me treatment in lamina propria dendritic cells (LPDCs) and macrophages, which play crucial roles in the humoral immune system. TLR5 expression correlated with the level of interleukin-6 (IL-6)/C–C chemokine type receptor 5, which are important mediators of the humoral immunity. Taken together, In vivo and ex vivo studies showed that EGCG3”Me potentiated the split-virus vaccine–elicited immune response accompanied with the upregulation of TLR5 in intestine and splenocyte macrophages.
format	article
author	Motofumi Kumazoe Kanako Takamatsu Fuyumi Horie Ren Yoshitomi Hiroki Hamagami Hiroshi Tanaka Yoshinori Fujimura Hirofumi Tachibana
author_facet	Motofumi Kumazoe Kanako Takamatsu Fuyumi Horie Ren Yoshitomi Hiroki Hamagami Hiroshi Tanaka Yoshinori Fujimura Hirofumi Tachibana
author_sort	Motofumi Kumazoe
title	Methylated (−)-epigallocatechin 3-O-gallate potentiates the effect of split vaccine accompanied with upregulation of Toll-like receptor 5
title_short	Methylated (−)-epigallocatechin 3-O-gallate potentiates the effect of split vaccine accompanied with upregulation of Toll-like receptor 5
title_full	Methylated (−)-epigallocatechin 3-O-gallate potentiates the effect of split vaccine accompanied with upregulation of Toll-like receptor 5
title_fullStr	Methylated (−)-epigallocatechin 3-O-gallate potentiates the effect of split vaccine accompanied with upregulation of Toll-like receptor 5
title_full_unstemmed	Methylated (−)-epigallocatechin 3-O-gallate potentiates the effect of split vaccine accompanied with upregulation of Toll-like receptor 5
title_sort	methylated (−)-epigallocatechin 3-o-gallate potentiates the effect of split vaccine accompanied with upregulation of toll-like receptor 5
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/7b8788c280364e29bde46c56c5696202
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Methylated (−)-epigallocatechin 3-O-gallate potentiates the effect of split vaccine accompanied with upregulation of Toll-like receptor 5

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