Integration of culture-based and molecular analysis of a complex sponge-associated bacterial community.

The bacterial communities of sponges have been studied using molecular techniques as well as culture-based techniques, but the communities described by these two methods are remarkably distinct. Culture-based methods describe communities dominated by Proteobacteria, and Actinomycetes while molecular...

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Autores principales:	Naomi F Montalvo, Jeanette Davis, Jan Vicente, Raquel Pittiglio, Jacques Ravel, Russell T Hill
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7b8c0644141947fa97a18e8807b92b43
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spelling	oai:doaj.org-article:7b8c0644141947fa97a18e8807b92b432021-11-18T08:28:43ZIntegration of culture-based and molecular analysis of a complex sponge-associated bacterial community.1932-620310.1371/journal.pone.0090517https://doaj.org/article/7b8c0644141947fa97a18e8807b92b432014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24618773/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203The bacterial communities of sponges have been studied using molecular techniques as well as culture-based techniques, but the communities described by these two methods are remarkably distinct. Culture-based methods describe communities dominated by Proteobacteria, and Actinomycetes while molecular methods describe communities dominated by predominantly uncultivated groups such as the Chloroflexi, Acidobacteria, and Acidimicrobidae. In this study, we used a wide range of culture media to increase the diversity of cultivable bacteria from the closely related giant barrel sponges, Xestospongia muta collected from the Florida Keys, Atlantic Ocean and Xestospongia testudinaria, collected from Indonesia, Pacific Ocean. Over 400 pure cultures were isolated and identified from X. muta and X. testudinaria and over 90 bacterial species were represented. Over 16,000 pyrosequences were analyzed and assigned to 976 OTUs. We employed both cultured-based methods and pyrosequencing to look for patterns of overlap between the culturable and molecular communities. Only one OTU was found in both the molecular and culturable communities, revealing limitations inherent in both approaches.Naomi F MontalvoJeanette DavisJan VicenteRaquel PittiglioJacques RavelRussell T HillPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 3, p e90517 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Naomi F Montalvo Jeanette Davis Jan Vicente Raquel Pittiglio Jacques Ravel Russell T Hill Integration of culture-based and molecular analysis of a complex sponge-associated bacterial community.
description	The bacterial communities of sponges have been studied using molecular techniques as well as culture-based techniques, but the communities described by these two methods are remarkably distinct. Culture-based methods describe communities dominated by Proteobacteria, and Actinomycetes while molecular methods describe communities dominated by predominantly uncultivated groups such as the Chloroflexi, Acidobacteria, and Acidimicrobidae. In this study, we used a wide range of culture media to increase the diversity of cultivable bacteria from the closely related giant barrel sponges, Xestospongia muta collected from the Florida Keys, Atlantic Ocean and Xestospongia testudinaria, collected from Indonesia, Pacific Ocean. Over 400 pure cultures were isolated and identified from X. muta and X. testudinaria and over 90 bacterial species were represented. Over 16,000 pyrosequences were analyzed and assigned to 976 OTUs. We employed both cultured-based methods and pyrosequencing to look for patterns of overlap between the culturable and molecular communities. Only one OTU was found in both the molecular and culturable communities, revealing limitations inherent in both approaches.
format	article
author	Naomi F Montalvo Jeanette Davis Jan Vicente Raquel Pittiglio Jacques Ravel Russell T Hill
author_facet	Naomi F Montalvo Jeanette Davis Jan Vicente Raquel Pittiglio Jacques Ravel Russell T Hill
author_sort	Naomi F Montalvo
title	Integration of culture-based and molecular analysis of a complex sponge-associated bacterial community.
title_short	Integration of culture-based and molecular analysis of a complex sponge-associated bacterial community.
title_full	Integration of culture-based and molecular analysis of a complex sponge-associated bacterial community.
title_fullStr	Integration of culture-based and molecular analysis of a complex sponge-associated bacterial community.
title_full_unstemmed	Integration of culture-based and molecular analysis of a complex sponge-associated bacterial community.
title_sort	integration of culture-based and molecular analysis of a complex sponge-associated bacterial community.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/7b8c0644141947fa97a18e8807b92b43
work_keys_str_mv	AT naomifmontalvo integrationofculturebasedandmolecularanalysisofacomplexspongeassociatedbacterialcommunity AT jeanettedavis integrationofculturebasedandmolecularanalysisofacomplexspongeassociatedbacterialcommunity AT janvicente integrationofculturebasedandmolecularanalysisofacomplexspongeassociatedbacterialcommunity AT raquelpittiglio integrationofculturebasedandmolecularanalysisofacomplexspongeassociatedbacterialcommunity AT jacquesravel integrationofculturebasedandmolecularanalysisofacomplexspongeassociatedbacterialcommunity AT russellthill integrationofculturebasedandmolecularanalysisofacomplexspongeassociatedbacterialcommunity
_version_	1718421749825011712

Integration of culture-based and molecular analysis of a complex sponge-associated bacterial community.

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