Highly efficient multiplex human T cell engineering without double-strand breaks using Cas9 base editors
Multiplexed genome engineering with Cas9 can increase efficiency but also the risk of unintended alterations. Here the authors demonstrate the use of multiplexed base editors on primary T cells with reduced translocation frequency.
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Nature Portfolio
2019
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oai:doaj.org-article:7b8f9319f4d349b9a42406e609bf1cd42021-12-02T16:57:43ZHighly efficient multiplex human T cell engineering without double-strand breaks using Cas9 base editors10.1038/s41467-019-13007-62041-1723https://doaj.org/article/7b8f9319f4d349b9a42406e609bf1cd42019-11-01T00:00:00Zhttps://doi.org/10.1038/s41467-019-13007-6https://doaj.org/toc/2041-1723Multiplexed genome engineering with Cas9 can increase efficiency but also the risk of unintended alterations. Here the authors demonstrate the use of multiplexed base editors on primary T cells with reduced translocation frequency.Beau R. WebberCara-lin LonetreeMitchell G. KluesnerMatthew J. JohnsonEmily J. PomeroyMiechaleen D. DiersWalker S. LahrGarrett M. DraperNicholas J. SlipekBranden A. SmeesterKlaus N. LovendahlAmber N. McElroyWendy R. GordonMark J. OsbornBranden S. MoriarityNature PortfolioarticleScienceQENNature Communications, Vol 10, Iss 1, Pp 1-10 (2019) |
institution |
DOAJ |
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DOAJ |
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EN |
topic |
Science Q |
spellingShingle |
Science Q Beau R. Webber Cara-lin Lonetree Mitchell G. Kluesner Matthew J. Johnson Emily J. Pomeroy Miechaleen D. Diers Walker S. Lahr Garrett M. Draper Nicholas J. Slipek Branden A. Smeester Klaus N. Lovendahl Amber N. McElroy Wendy R. Gordon Mark J. Osborn Branden S. Moriarity Highly efficient multiplex human T cell engineering without double-strand breaks using Cas9 base editors |
description |
Multiplexed genome engineering with Cas9 can increase efficiency but also the risk of unintended alterations. Here the authors demonstrate the use of multiplexed base editors on primary T cells with reduced translocation frequency. |
format |
article |
author |
Beau R. Webber Cara-lin Lonetree Mitchell G. Kluesner Matthew J. Johnson Emily J. Pomeroy Miechaleen D. Diers Walker S. Lahr Garrett M. Draper Nicholas J. Slipek Branden A. Smeester Klaus N. Lovendahl Amber N. McElroy Wendy R. Gordon Mark J. Osborn Branden S. Moriarity |
author_facet |
Beau R. Webber Cara-lin Lonetree Mitchell G. Kluesner Matthew J. Johnson Emily J. Pomeroy Miechaleen D. Diers Walker S. Lahr Garrett M. Draper Nicholas J. Slipek Branden A. Smeester Klaus N. Lovendahl Amber N. McElroy Wendy R. Gordon Mark J. Osborn Branden S. Moriarity |
author_sort |
Beau R. Webber |
title |
Highly efficient multiplex human T cell engineering without double-strand breaks using Cas9 base editors |
title_short |
Highly efficient multiplex human T cell engineering without double-strand breaks using Cas9 base editors |
title_full |
Highly efficient multiplex human T cell engineering without double-strand breaks using Cas9 base editors |
title_fullStr |
Highly efficient multiplex human T cell engineering without double-strand breaks using Cas9 base editors |
title_full_unstemmed |
Highly efficient multiplex human T cell engineering without double-strand breaks using Cas9 base editors |
title_sort |
highly efficient multiplex human t cell engineering without double-strand breaks using cas9 base editors |
publisher |
Nature Portfolio |
publishDate |
2019 |
url |
https://doaj.org/article/7b8f9319f4d349b9a42406e609bf1cd4 |
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