Highly efficient multiplex human T cell engineering without double-strand breaks using Cas9 base editors

Multiplexed genome engineering with Cas9 can increase efficiency but also the risk of unintended alterations. Here the authors demonstrate the use of multiplexed base editors on primary T cells with reduced translocation frequency.

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Detalles Bibliográficos
Autores principales:	Beau R. Webber, Cara-lin Lonetree, Mitchell G. Kluesner, Matthew J. Johnson, Emily J. Pomeroy, Miechaleen D. Diers, Walker S. Lahr, Garrett M. Draper, Nicholas J. Slipek, Branden A. Smeester, Klaus N. Lovendahl, Amber N. McElroy, Wendy R. Gordon, Mark J. Osborn, Branden S. Moriarity
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/7b8f9319f4d349b9a42406e609bf1cd4
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spelling	oai:doaj.org-article:7b8f9319f4d349b9a42406e609bf1cd42021-12-02T16:57:43ZHighly efficient multiplex human T cell engineering without double-strand breaks using Cas9 base editors10.1038/s41467-019-13007-62041-1723https://doaj.org/article/7b8f9319f4d349b9a42406e609bf1cd42019-11-01T00:00:00Zhttps://doi.org/10.1038/s41467-019-13007-6https://doaj.org/toc/2041-1723Multiplexed genome engineering with Cas9 can increase efficiency but also the risk of unintended alterations. Here the authors demonstrate the use of multiplexed base editors on primary T cells with reduced translocation frequency.Beau R. WebberCara-lin LonetreeMitchell G. KluesnerMatthew J. JohnsonEmily J. PomeroyMiechaleen D. DiersWalker S. LahrGarrett M. DraperNicholas J. SlipekBranden A. SmeesterKlaus N. LovendahlAmber N. McElroyWendy R. GordonMark J. OsbornBranden S. MoriarityNature PortfolioarticleScienceQENNature Communications, Vol 10, Iss 1, Pp 1-10 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Beau R. Webber Cara-lin Lonetree Mitchell G. Kluesner Matthew J. Johnson Emily J. Pomeroy Miechaleen D. Diers Walker S. Lahr Garrett M. Draper Nicholas J. Slipek Branden A. Smeester Klaus N. Lovendahl Amber N. McElroy Wendy R. Gordon Mark J. Osborn Branden S. Moriarity Highly efficient multiplex human T cell engineering without double-strand breaks using Cas9 base editors
description	Multiplexed genome engineering with Cas9 can increase efficiency but also the risk of unintended alterations. Here the authors demonstrate the use of multiplexed base editors on primary T cells with reduced translocation frequency.
format	article
author	Beau R. Webber Cara-lin Lonetree Mitchell G. Kluesner Matthew J. Johnson Emily J. Pomeroy Miechaleen D. Diers Walker S. Lahr Garrett M. Draper Nicholas J. Slipek Branden A. Smeester Klaus N. Lovendahl Amber N. McElroy Wendy R. Gordon Mark J. Osborn Branden S. Moriarity
author_facet	Beau R. Webber Cara-lin Lonetree Mitchell G. Kluesner Matthew J. Johnson Emily J. Pomeroy Miechaleen D. Diers Walker S. Lahr Garrett M. Draper Nicholas J. Slipek Branden A. Smeester Klaus N. Lovendahl Amber N. McElroy Wendy R. Gordon Mark J. Osborn Branden S. Moriarity
author_sort	Beau R. Webber
title	Highly efficient multiplex human T cell engineering without double-strand breaks using Cas9 base editors
title_short	Highly efficient multiplex human T cell engineering without double-strand breaks using Cas9 base editors
title_full	Highly efficient multiplex human T cell engineering without double-strand breaks using Cas9 base editors
title_fullStr	Highly efficient multiplex human T cell engineering without double-strand breaks using Cas9 base editors
title_full_unstemmed	Highly efficient multiplex human T cell engineering without double-strand breaks using Cas9 base editors
title_sort	highly efficient multiplex human t cell engineering without double-strand breaks using cas9 base editors
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/7b8f9319f4d349b9a42406e609bf1cd4
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Highly efficient multiplex human T cell engineering without double-strand breaks using Cas9 base editors

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