Highly efficient multiplex human T cell engineering without double-strand breaks using Cas9 base editors

Multiplexed genome engineering with Cas9 can increase efficiency but also the risk of unintended alterations. Here the authors demonstrate the use of multiplexed base editors on primary T cells with reduced translocation frequency.

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Autores principales: Beau R. Webber, Cara-lin Lonetree, Mitchell G. Kluesner, Matthew J. Johnson, Emily J. Pomeroy, Miechaleen D. Diers, Walker S. Lahr, Garrett M. Draper, Nicholas J. Slipek, Branden A. Smeester, Klaus N. Lovendahl, Amber N. McElroy, Wendy R. Gordon, Mark J. Osborn, Branden S. Moriarity
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Publicado: Nature Portfolio 2019
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Acceso en línea:https://doaj.org/article/7b8f9319f4d349b9a42406e609bf1cd4
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spelling oai:doaj.org-article:7b8f9319f4d349b9a42406e609bf1cd42021-12-02T16:57:43ZHighly efficient multiplex human T cell engineering without double-strand breaks using Cas9 base editors10.1038/s41467-019-13007-62041-1723https://doaj.org/article/7b8f9319f4d349b9a42406e609bf1cd42019-11-01T00:00:00Zhttps://doi.org/10.1038/s41467-019-13007-6https://doaj.org/toc/2041-1723Multiplexed genome engineering with Cas9 can increase efficiency but also the risk of unintended alterations. Here the authors demonstrate the use of multiplexed base editors on primary T cells with reduced translocation frequency.Beau R. WebberCara-lin LonetreeMitchell G. KluesnerMatthew J. JohnsonEmily J. PomeroyMiechaleen D. DiersWalker S. LahrGarrett M. DraperNicholas J. SlipekBranden A. SmeesterKlaus N. LovendahlAmber N. McElroyWendy R. GordonMark J. OsbornBranden S. MoriarityNature PortfolioarticleScienceQENNature Communications, Vol 10, Iss 1, Pp 1-10 (2019)
institution DOAJ
collection DOAJ
language EN
topic Science
Q
spellingShingle Science
Q
Beau R. Webber
Cara-lin Lonetree
Mitchell G. Kluesner
Matthew J. Johnson
Emily J. Pomeroy
Miechaleen D. Diers
Walker S. Lahr
Garrett M. Draper
Nicholas J. Slipek
Branden A. Smeester
Klaus N. Lovendahl
Amber N. McElroy
Wendy R. Gordon
Mark J. Osborn
Branden S. Moriarity
Highly efficient multiplex human T cell engineering without double-strand breaks using Cas9 base editors
description Multiplexed genome engineering with Cas9 can increase efficiency but also the risk of unintended alterations. Here the authors demonstrate the use of multiplexed base editors on primary T cells with reduced translocation frequency.
format article
author Beau R. Webber
Cara-lin Lonetree
Mitchell G. Kluesner
Matthew J. Johnson
Emily J. Pomeroy
Miechaleen D. Diers
Walker S. Lahr
Garrett M. Draper
Nicholas J. Slipek
Branden A. Smeester
Klaus N. Lovendahl
Amber N. McElroy
Wendy R. Gordon
Mark J. Osborn
Branden S. Moriarity
author_facet Beau R. Webber
Cara-lin Lonetree
Mitchell G. Kluesner
Matthew J. Johnson
Emily J. Pomeroy
Miechaleen D. Diers
Walker S. Lahr
Garrett M. Draper
Nicholas J. Slipek
Branden A. Smeester
Klaus N. Lovendahl
Amber N. McElroy
Wendy R. Gordon
Mark J. Osborn
Branden S. Moriarity
author_sort Beau R. Webber
title Highly efficient multiplex human T cell engineering without double-strand breaks using Cas9 base editors
title_short Highly efficient multiplex human T cell engineering without double-strand breaks using Cas9 base editors
title_full Highly efficient multiplex human T cell engineering without double-strand breaks using Cas9 base editors
title_fullStr Highly efficient multiplex human T cell engineering without double-strand breaks using Cas9 base editors
title_full_unstemmed Highly efficient multiplex human T cell engineering without double-strand breaks using Cas9 base editors
title_sort highly efficient multiplex human t cell engineering without double-strand breaks using cas9 base editors
publisher Nature Portfolio
publishDate 2019
url https://doaj.org/article/7b8f9319f4d349b9a42406e609bf1cd4
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