Synthesis, characterization, and efficacy of antimicrobial chlorhexidine hexametaphosphate nanoparticles for applications in biomedical materials and consumer products

Michele E Barbour,1 Sarah E Maddocks,2 Natalie J Wood,1,3 Andrew M Collins3 1Oral Nanoscience, School of Oral and Dental Sciences, University of Bristol, Bristol, UK; 2Cardiff School of Health Sciences, Cardiff Metropolitan University, Cardiff, UK; 3Bristol Centre for Functional Nanomaterials, Unive...

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Autores principales:	Barbour ME, Maddocks SE, Wood NJ, Collins AM
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2013
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/7b945d6ca68341eea8320c1eefec7531
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spelling	oai:doaj.org-article:7b945d6ca68341eea8320c1eefec75312021-12-02T02:44:07ZSynthesis, characterization, and efficacy of antimicrobial chlorhexidine hexametaphosphate nanoparticles for applications in biomedical materials and consumer products1176-91141178-2013https://doaj.org/article/7b945d6ca68341eea8320c1eefec75312013-09-01T00:00:00Zhttp://www.dovepress.com/synthesis-characterization-and-efficacy-of-antimicrobial-chlorhexidine-a14418https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Michele E Barbour,1 Sarah E Maddocks,2 Natalie J Wood,1,3 Andrew M Collins3 1Oral Nanoscience, School of Oral and Dental Sciences, University of Bristol, Bristol, UK; 2Cardiff School of Health Sciences, Cardiff Metropolitan University, Cardiff, UK; 3Bristol Centre for Functional Nanomaterials, University of Bristol, Bristol, UK Abstract: Chlorhexidine (CHX) is an antimicrobial agent that is efficacious against gram-negative and -positive bacteria and yeasts. Its mechanism of action is based on cell membrane disruption and, as such, it does not promote the development of bacterial resistance, which is associated with the widespread use of antibiotics. In this manuscript, we report the development of novel antimicrobial nanoparticles (NPs) based on a hexametaphosphate salt of CHX. These are synthesized by instantaneous reaction between equimolar aqueous solutions of CHX digluconate and sodium hexametaphosphate, under room temperature and pressure. The reaction results in a stable colloid composed of highly negatively charged NPs (−50 mV), of size 20-160 nm. The NPs adhere rapidly to specimens of glass, titanium, and an elastomeric wound dressing, in a dose-dependent manner. The functionalized materials exhibit a gradual leaching of soluble CHX over a period of at least 50 days. The NP colloid is efficacious against methicillin-resistant Staphylococcus aureus (MRSA) and Pseudomonas aeruginosa in both planktonic and biofilm conditions. These NPs may find application in a range of biomedical and consumer materials. Keywords: MRSA, biomaterials, chlorhexidine, drug delivery, slow releaseBarbour MEMaddocks SEWood NJCollins AMDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2013, Iss default, Pp 3507-3519 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Barbour ME Maddocks SE Wood NJ Collins AM Synthesis, characterization, and efficacy of antimicrobial chlorhexidine hexametaphosphate nanoparticles for applications in biomedical materials and consumer products
description	Michele E Barbour,1 Sarah E Maddocks,2 Natalie J Wood,1,3 Andrew M Collins3 1Oral Nanoscience, School of Oral and Dental Sciences, University of Bristol, Bristol, UK; 2Cardiff School of Health Sciences, Cardiff Metropolitan University, Cardiff, UK; 3Bristol Centre for Functional Nanomaterials, University of Bristol, Bristol, UK Abstract: Chlorhexidine (CHX) is an antimicrobial agent that is efficacious against gram-negative and -positive bacteria and yeasts. Its mechanism of action is based on cell membrane disruption and, as such, it does not promote the development of bacterial resistance, which is associated with the widespread use of antibiotics. In this manuscript, we report the development of novel antimicrobial nanoparticles (NPs) based on a hexametaphosphate salt of CHX. These are synthesized by instantaneous reaction between equimolar aqueous solutions of CHX digluconate and sodium hexametaphosphate, under room temperature and pressure. The reaction results in a stable colloid composed of highly negatively charged NPs (−50 mV), of size 20-160 nm. The NPs adhere rapidly to specimens of glass, titanium, and an elastomeric wound dressing, in a dose-dependent manner. The functionalized materials exhibit a gradual leaching of soluble CHX over a period of at least 50 days. The NP colloid is efficacious against methicillin-resistant Staphylococcus aureus (MRSA) and Pseudomonas aeruginosa in both planktonic and biofilm conditions. These NPs may find application in a range of biomedical and consumer materials. Keywords: MRSA, biomaterials, chlorhexidine, drug delivery, slow release
format	article
author	Barbour ME Maddocks SE Wood NJ Collins AM
author_facet	Barbour ME Maddocks SE Wood NJ Collins AM
author_sort	Barbour ME
title	Synthesis, characterization, and efficacy of antimicrobial chlorhexidine hexametaphosphate nanoparticles for applications in biomedical materials and consumer products
title_short	Synthesis, characterization, and efficacy of antimicrobial chlorhexidine hexametaphosphate nanoparticles for applications in biomedical materials and consumer products
title_full	Synthesis, characterization, and efficacy of antimicrobial chlorhexidine hexametaphosphate nanoparticles for applications in biomedical materials and consumer products
title_fullStr	Synthesis, characterization, and efficacy of antimicrobial chlorhexidine hexametaphosphate nanoparticles for applications in biomedical materials and consumer products
title_full_unstemmed	Synthesis, characterization, and efficacy of antimicrobial chlorhexidine hexametaphosphate nanoparticles for applications in biomedical materials and consumer products
title_sort	synthesis, characterization, and efficacy of antimicrobial chlorhexidine hexametaphosphate nanoparticles for applications in biomedical materials and consumer products
publisher	Dove Medical Press
publishDate	2013
url	https://doaj.org/article/7b945d6ca68341eea8320c1eefec7531
work_keys_str_mv	AT barbourme synthesischaracterizationandefficacyofantimicrobialchlorhexidinehexametaphosphatenanoparticlesforapplicationsinbiomedicalmaterialsandconsumerproducts AT maddocksse synthesischaracterizationandefficacyofantimicrobialchlorhexidinehexametaphosphatenanoparticlesforapplicationsinbiomedicalmaterialsandconsumerproducts AT woodnj synthesischaracterizationandefficacyofantimicrobialchlorhexidinehexametaphosphatenanoparticlesforapplicationsinbiomedicalmaterialsandconsumerproducts AT collinsam synthesischaracterizationandefficacyofantimicrobialchlorhexidinehexametaphosphatenanoparticlesforapplicationsinbiomedicalmaterialsandconsumerproducts
_version_	1718402171787018240

Synthesis, characterization, and efficacy of antimicrobial chlorhexidine hexametaphosphate nanoparticles for applications in biomedical materials and consumer products

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