Schisandrae fructus enhances myogenic differentiation and inhibits atrophy through protein synthesis in human myotubes

Cy Hyun Kim,1,2,* Jin-Hong Shin,1,3,* Sung Jun Hwang,1,2 Yung Hyun Choi,4 Dae-Seong Kim,1,3 Cheol Min Kim2,51Research Institute of Convergence of Biomedical Science and Technology, Pusan National University Yangsan Hospital, Yangsan, 2Center for Anti-Aging Industry, Pusan National University, Busan,...

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Autores principales:	Kim CH, Shin JH, Hwang SJ, Choi YH, Kim DS, Kim CM
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2016
Materias:	Schisandrae fructus mTOR signaling Human skeletal muscle cells Myotube Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/7b96097784b54f92a71dd231a5d8bcb6
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spelling	oai:doaj.org-article:7b96097784b54f92a71dd231a5d8bcb62021-12-02T03:58:38ZSchisandrae fructus enhances myogenic differentiation and inhibits atrophy through protein synthesis in human myotubes1178-2013https://doaj.org/article/7b96097784b54f92a71dd231a5d8bcb62016-05-01T00:00:00Zhttps://www.dovepress.com/schisandrae-fructus-enhances-myogenic-differentiation-and-inhibits-atr-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Cy Hyun Kim,1,2,* Jin-Hong Shin,1,3,* Sung Jun Hwang,1,2 Yung Hyun Choi,4 Dae-Seong Kim,1,3 Cheol Min Kim2,51Research Institute of Convergence of Biomedical Science and Technology, Pusan National University Yangsan Hospital, Yangsan, 2Center for Anti-Aging Industry, Pusan National University, Busan, 3Department of Neurology, Pusan National University Yangsan Hospital, Yangsan, 4Department of Biochemistry, Dong-eui University College of Korean Medicine, Busan, 5Department of Biomedical Informatics, Pusan National University School of Medicine, Yangsan, Republic of Korea*These authors contributed equally to this work Abstract: Schisandrae fructus (SF) has recently been reported to increase skeletal muscle mass and inhibit atrophy in mice. We investigated the effect of SF extract on human myotube differentiation and its acting pathway. Various concentrations (0.1–10 µg/mL) of SF extract were applied on human skeletal muscle cells in vitro. Myotube area and fusion index were measured to quantify myotube differentiation. The maximum effect was observed at 0.5 µg/mL of SF extract, enhancing differentiation up to 1.4-fold in fusion index and 1.6-fold in myotube area at 8 days after induction of differentiation compared to control. Phosphorylation of eukaryotic translation initiation factor 4E-binding protein 1 and 70 kDa ribosomal protein S6 kinase, which initiate translation as downstream of mammalian target of rapamycin pathway, was upregulated in early phases of differentiation after SF treatment. SF also attenuated dexamethasone-induced atrophy. In conclusion, we show that SF augments myogenic differentiation and attenuates atrophy by increasing protein synthesis through mammalian target of rapamycin/70 kDa ribosomal protein S6 kinase and eukaryotic translation initiation factor 4E-binding protein 1 signaling pathway in human myotubes. SF can be a useful natural dietary supplement in increasing skeletal muscle mass, especially in the aged with sarcopenia and the patients with disuse atrophy.Keywords: Schisandrae fructus, mTOR signaling, human skeletal muscle cells, myotubesKim CHShin JHHwang SJChoi YHKim DSKim CMDove Medical PressarticleSchisandrae fructusmTOR signalingHuman skeletal muscle cellsMyotubeMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2016, Iss default, Pp 2407-2415 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	Schisandrae fructus mTOR signaling Human skeletal muscle cells Myotube Medicine (General) R5-920
spellingShingle	Schisandrae fructus mTOR signaling Human skeletal muscle cells Myotube Medicine (General) R5-920 Kim CH Shin JH Hwang SJ Choi YH Kim DS Kim CM Schisandrae fructus enhances myogenic differentiation and inhibits atrophy through protein synthesis in human myotubes
description	Cy Hyun Kim,1,2,* Jin-Hong Shin,1,3,* Sung Jun Hwang,1,2 Yung Hyun Choi,4 Dae-Seong Kim,1,3 Cheol Min Kim2,51Research Institute of Convergence of Biomedical Science and Technology, Pusan National University Yangsan Hospital, Yangsan, 2Center for Anti-Aging Industry, Pusan National University, Busan, 3Department of Neurology, Pusan National University Yangsan Hospital, Yangsan, 4Department of Biochemistry, Dong-eui University College of Korean Medicine, Busan, 5Department of Biomedical Informatics, Pusan National University School of Medicine, Yangsan, Republic of Korea*These authors contributed equally to this work Abstract: Schisandrae fructus (SF) has recently been reported to increase skeletal muscle mass and inhibit atrophy in mice. We investigated the effect of SF extract on human myotube differentiation and its acting pathway. Various concentrations (0.1–10 µg/mL) of SF extract were applied on human skeletal muscle cells in vitro. Myotube area and fusion index were measured to quantify myotube differentiation. The maximum effect was observed at 0.5 µg/mL of SF extract, enhancing differentiation up to 1.4-fold in fusion index and 1.6-fold in myotube area at 8 days after induction of differentiation compared to control. Phosphorylation of eukaryotic translation initiation factor 4E-binding protein 1 and 70 kDa ribosomal protein S6 kinase, which initiate translation as downstream of mammalian target of rapamycin pathway, was upregulated in early phases of differentiation after SF treatment. SF also attenuated dexamethasone-induced atrophy. In conclusion, we show that SF augments myogenic differentiation and attenuates atrophy by increasing protein synthesis through mammalian target of rapamycin/70 kDa ribosomal protein S6 kinase and eukaryotic translation initiation factor 4E-binding protein 1 signaling pathway in human myotubes. SF can be a useful natural dietary supplement in increasing skeletal muscle mass, especially in the aged with sarcopenia and the patients with disuse atrophy.Keywords: Schisandrae fructus, mTOR signaling, human skeletal muscle cells, myotubes
format	article
author	Kim CH Shin JH Hwang SJ Choi YH Kim DS Kim CM
author_facet	Kim CH Shin JH Hwang SJ Choi YH Kim DS Kim CM
author_sort	Kim CH
title	Schisandrae fructus enhances myogenic differentiation and inhibits atrophy through protein synthesis in human myotubes
title_short	Schisandrae fructus enhances myogenic differentiation and inhibits atrophy through protein synthesis in human myotubes
title_full	Schisandrae fructus enhances myogenic differentiation and inhibits atrophy through protein synthesis in human myotubes
title_fullStr	Schisandrae fructus enhances myogenic differentiation and inhibits atrophy through protein synthesis in human myotubes
title_full_unstemmed	Schisandrae fructus enhances myogenic differentiation and inhibits atrophy through protein synthesis in human myotubes
title_sort	schisandrae fructus enhances myogenic differentiation and inhibits atrophy through protein synthesis in human myotubes
publisher	Dove Medical Press
publishDate	2016
url	https://doaj.org/article/7b96097784b54f92a71dd231a5d8bcb6
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_version_	1718401499029045248

Schisandrae fructus enhances myogenic differentiation and inhibits atrophy through protein synthesis in human myotubes

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