A global analysis of tandem 3'UTRs in eosinophilic chronic rhinosinusitis with nasal polyps.

<h4>Background</h4>Alternative polyadenylation (APA) is emerging as a widespread mechanism of gene regulation. The usage of APA sites allows a single gene to encode multiple mRNA transcripts with different 3'-untranslated region (3'UTR) lengths. Many disease processes reflect t...

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Autores principales:	Peng Tian, Yu Sun, Yuxin Li, Xiang Liu, Liang Wan, Jie Li, Yun Ma, Anlong Xu, Yonggui Fu, Hua Zou
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7b97b3f227f84befaafa20c73ffa8bd7
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spelling	oai:doaj.org-article:7b97b3f227f84befaafa20c73ffa8bd72021-11-18T08:08:19ZA global analysis of tandem 3'UTRs in eosinophilic chronic rhinosinusitis with nasal polyps.1932-620310.1371/journal.pone.0048997https://doaj.org/article/7b97b3f227f84befaafa20c73ffa8bd72012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23185289/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Alternative polyadenylation (APA) is emerging as a widespread mechanism of gene regulation. The usage of APA sites allows a single gene to encode multiple mRNA transcripts with different 3'-untranslated region (3'UTR) lengths. Many disease processes reflect the importance of the regulation of APA site switching. The objective of this study was to explore the profiling of tandem APA sites in nasal polyps compared with nasal uncinate process mucosa.<h4>Methods</h4>Sequencing of APA sites (SAPAS) based on second-generation sequencing technology was undertaken to investigate the use of tandem APA sites and identify gene expression patterns in samples from the nasal polyps and nasal uncinate process mucosa of two patients with chronic rhinosinusitis with nasal polyps. The findings of the SAPAS analysis were validated via quantitative reverse-transcription polymerase chain reaction (qRT-PCR).<h4>Results</h4>First, the results showed a switching of 3'UTR lengths in nasal polyps compared with nasal uncinate process mucosa. From the two patients, 105 genes that were detected in both patients in the nasal polyps were switched to distal poly(A) sites, and 90 such genes were switched to proximal poly(A) sites. Several Gene Ontology terms were enriched in the list of genes with switched APA sites, including transcription regulation, cell cycle, apoptosis, and metabolism. Second, we detected genes that showed differential expression with at least a 3-fold difference between nasal polyp tissue and nasal uncinate process mucosa. Between the two sample types, 627 genes exhibited differential expression. The qRT-PCR results confirmed our SAPAS results.<h4>Conclusion</h4>APA site-switching events of 3'UTRs are prevalent in nasal polyp tissue, and the regulation of gene expression mediated by APA may play an important role in the formation and persistence of nasal polyps. Our results may provide new insights into the possible pathophysiologic processes involved in nasal polyps.Peng TianYu SunYuxin LiXiang LiuLiang WanJie LiYun MaAnlong XuYonggui FuHua ZouPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 11, p e48997 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Peng Tian Yu Sun Yuxin Li Xiang Liu Liang Wan Jie Li Yun Ma Anlong Xu Yonggui Fu Hua Zou A global analysis of tandem 3'UTRs in eosinophilic chronic rhinosinusitis with nasal polyps.
description	<h4>Background</h4>Alternative polyadenylation (APA) is emerging as a widespread mechanism of gene regulation. The usage of APA sites allows a single gene to encode multiple mRNA transcripts with different 3'-untranslated region (3'UTR) lengths. Many disease processes reflect the importance of the regulation of APA site switching. The objective of this study was to explore the profiling of tandem APA sites in nasal polyps compared with nasal uncinate process mucosa.<h4>Methods</h4>Sequencing of APA sites (SAPAS) based on second-generation sequencing technology was undertaken to investigate the use of tandem APA sites and identify gene expression patterns in samples from the nasal polyps and nasal uncinate process mucosa of two patients with chronic rhinosinusitis with nasal polyps. The findings of the SAPAS analysis were validated via quantitative reverse-transcription polymerase chain reaction (qRT-PCR).<h4>Results</h4>First, the results showed a switching of 3'UTR lengths in nasal polyps compared with nasal uncinate process mucosa. From the two patients, 105 genes that were detected in both patients in the nasal polyps were switched to distal poly(A) sites, and 90 such genes were switched to proximal poly(A) sites. Several Gene Ontology terms were enriched in the list of genes with switched APA sites, including transcription regulation, cell cycle, apoptosis, and metabolism. Second, we detected genes that showed differential expression with at least a 3-fold difference between nasal polyp tissue and nasal uncinate process mucosa. Between the two sample types, 627 genes exhibited differential expression. The qRT-PCR results confirmed our SAPAS results.<h4>Conclusion</h4>APA site-switching events of 3'UTRs are prevalent in nasal polyp tissue, and the regulation of gene expression mediated by APA may play an important role in the formation and persistence of nasal polyps. Our results may provide new insights into the possible pathophysiologic processes involved in nasal polyps.
format	article
author	Peng Tian Yu Sun Yuxin Li Xiang Liu Liang Wan Jie Li Yun Ma Anlong Xu Yonggui Fu Hua Zou
author_facet	Peng Tian Yu Sun Yuxin Li Xiang Liu Liang Wan Jie Li Yun Ma Anlong Xu Yonggui Fu Hua Zou
author_sort	Peng Tian
title	A global analysis of tandem 3'UTRs in eosinophilic chronic rhinosinusitis with nasal polyps.
title_short	A global analysis of tandem 3'UTRs in eosinophilic chronic rhinosinusitis with nasal polyps.
title_full	A global analysis of tandem 3'UTRs in eosinophilic chronic rhinosinusitis with nasal polyps.
title_fullStr	A global analysis of tandem 3'UTRs in eosinophilic chronic rhinosinusitis with nasal polyps.
title_full_unstemmed	A global analysis of tandem 3'UTRs in eosinophilic chronic rhinosinusitis with nasal polyps.
title_sort	global analysis of tandem 3'utrs in eosinophilic chronic rhinosinusitis with nasal polyps.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/7b97b3f227f84befaafa20c73ffa8bd7
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A global analysis of tandem 3'UTRs in eosinophilic chronic rhinosinusitis with nasal polyps.

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