Involvement of autophagy in tantalum nanoparticle-induced osteoblast proliferation

Chengrong Kang,1,2 Limin Wei,1 Bin Song,1 Liangjiao Chen,3 Jia Liu,1 Bin Deng,4 Xuan Pan,2 Longquan Shao1 1Department of Stomatology, Nanfang Hospital, Southern Medical University, 2Department of Stomatology, The First Affiliated Hospital of Guangdong Pharmaceutical University, 3Department of Ortho...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Kang C, Wei L, Song B, Chen L, Liu J, Deng B, Pan X, Shao L
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2017
Materias:	tantalum nanoparticles (Ta-NPs) osteoblast autophagy proliferation Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/7b9b1a163a3e43d18070464a82ac7a7f
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:7b9b1a163a3e43d18070464a82ac7a7f
record_format	dspace
spelling	oai:doaj.org-article:7b9b1a163a3e43d18070464a82ac7a7f2021-12-02T01:34:51ZInvolvement of autophagy in tantalum nanoparticle-induced osteoblast proliferation1178-2013https://doaj.org/article/7b9b1a163a3e43d18070464a82ac7a7f2017-06-01T00:00:00Zhttps://www.dovepress.com/involvement-of-autophagy-in-tantalum-nanoparticle-induced-osteoblast-p-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Chengrong Kang,1,2 Limin Wei,1 Bin Song,1 Liangjiao Chen,3 Jia Liu,1 Bin Deng,4 Xuan Pan,2 Longquan Shao1 1Department of Stomatology, Nanfang Hospital, Southern Medical University, 2Department of Stomatology, The First Affiliated Hospital of Guangdong Pharmaceutical University, 3Department of Orthodontics, Key Laboratory of Oral Medicine, Guangzhou Institute of Oral Disease, Stomatology Hospital of Guangzhou Medical University, Guangzhou, 4Department of Stomatology, The General Hospital of People’s Liberation Army, Beijing, China Abstract: Porous tantalum (Ta) implants are highly corrosion resistant and biocompatible, and they possess significantly better initial stability than that of conventional titanium (Ti) implants. During loading wear, Ta nanoparticles (Ta-NPs) that were deposited on the surface of a porous Ta implant are inevitably released and come into direct contact with peri-implant osteoblasts. The wear debris may influence cell behavior and implant stabilization. However, the interaction of Ta-NPs with osteoblasts has not been clearly investigated. This study aimed to investigate the effect of Ta-NPs on cell proliferation and their underlying mechanism. The Cell Counting Kit-8 (CCK-8) assay was used to measure the cell viability of MC3T3-E1 mouse osteoblasts and showed that Ta-NP treatment could increase cell viability. Then, confocal microscopy, Western blotting, and transmission electron microscopy were used to confirm the autophagy induced by Ta-NPs, and evidence of autophagy induction was observed as positive LC3 puncta, high-LC3-II expression, and autophagic vesicle ultrastructures. The CCK-8 assay revealed that the cell viability was further increased and decreased by the application of an autophagy inducer and inhibitor, respectively. In addition, pre-treatment with autophagy inhibitor 3-methyladenine (3-MA) inhibited the Ta-NP-induced autophagy. These results indicate that the Ta-NPs can promote cell proliferation, that an autophagy inducer can further strengthen this effect and that an autophagy inhibitor can weaken this effect. In conclusion, autophagy was involved in Ta-NP-induced cell proliferation and had a promoting effect. Keywords: tantalum nanoparticles, osteoblast, autophagy, proliferation Kang CWei LSong BChen LLiu JDeng BPan XShao LDove Medical Pressarticletantalum nanoparticles (Ta-NPs)osteoblastautophagyproliferationMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 12, Pp 4323-4333 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	tantalum nanoparticles (Ta-NPs) osteoblast autophagy proliferation Medicine (General) R5-920
spellingShingle	tantalum nanoparticles (Ta-NPs) osteoblast autophagy proliferation Medicine (General) R5-920 Kang C Wei L Song B Chen L Liu J Deng B Pan X Shao L Involvement of autophagy in tantalum nanoparticle-induced osteoblast proliferation
description	Chengrong Kang,1,2 Limin Wei,1 Bin Song,1 Liangjiao Chen,3 Jia Liu,1 Bin Deng,4 Xuan Pan,2 Longquan Shao1 1Department of Stomatology, Nanfang Hospital, Southern Medical University, 2Department of Stomatology, The First Affiliated Hospital of Guangdong Pharmaceutical University, 3Department of Orthodontics, Key Laboratory of Oral Medicine, Guangzhou Institute of Oral Disease, Stomatology Hospital of Guangzhou Medical University, Guangzhou, 4Department of Stomatology, The General Hospital of People’s Liberation Army, Beijing, China Abstract: Porous tantalum (Ta) implants are highly corrosion resistant and biocompatible, and they possess significantly better initial stability than that of conventional titanium (Ti) implants. During loading wear, Ta nanoparticles (Ta-NPs) that were deposited on the surface of a porous Ta implant are inevitably released and come into direct contact with peri-implant osteoblasts. The wear debris may influence cell behavior and implant stabilization. However, the interaction of Ta-NPs with osteoblasts has not been clearly investigated. This study aimed to investigate the effect of Ta-NPs on cell proliferation and their underlying mechanism. The Cell Counting Kit-8 (CCK-8) assay was used to measure the cell viability of MC3T3-E1 mouse osteoblasts and showed that Ta-NP treatment could increase cell viability. Then, confocal microscopy, Western blotting, and transmission electron microscopy were used to confirm the autophagy induced by Ta-NPs, and evidence of autophagy induction was observed as positive LC3 puncta, high-LC3-II expression, and autophagic vesicle ultrastructures. The CCK-8 assay revealed that the cell viability was further increased and decreased by the application of an autophagy inducer and inhibitor, respectively. In addition, pre-treatment with autophagy inhibitor 3-methyladenine (3-MA) inhibited the Ta-NP-induced autophagy. These results indicate that the Ta-NPs can promote cell proliferation, that an autophagy inducer can further strengthen this effect and that an autophagy inhibitor can weaken this effect. In conclusion, autophagy was involved in Ta-NP-induced cell proliferation and had a promoting effect. Keywords: tantalum nanoparticles, osteoblast, autophagy, proliferation
format	article
author	Kang C Wei L Song B Chen L Liu J Deng B Pan X Shao L
author_facet	Kang C Wei L Song B Chen L Liu J Deng B Pan X Shao L
author_sort	Kang C
title	Involvement of autophagy in tantalum nanoparticle-induced osteoblast proliferation
title_short	Involvement of autophagy in tantalum nanoparticle-induced osteoblast proliferation
title_full	Involvement of autophagy in tantalum nanoparticle-induced osteoblast proliferation
title_fullStr	Involvement of autophagy in tantalum nanoparticle-induced osteoblast proliferation
title_full_unstemmed	Involvement of autophagy in tantalum nanoparticle-induced osteoblast proliferation
title_sort	involvement of autophagy in tantalum nanoparticle-induced osteoblast proliferation
publisher	Dove Medical Press
publishDate	2017
url	https://doaj.org/article/7b9b1a163a3e43d18070464a82ac7a7f
work_keys_str_mv	AT kangc involvementofautophagyintantalumnanoparticleinducedosteoblastproliferation AT weil involvementofautophagyintantalumnanoparticleinducedosteoblastproliferation AT songb involvementofautophagyintantalumnanoparticleinducedosteoblastproliferation AT chenl involvementofautophagyintantalumnanoparticleinducedosteoblastproliferation AT liuj involvementofautophagyintantalumnanoparticleinducedosteoblastproliferation AT dengb involvementofautophagyintantalumnanoparticleinducedosteoblastproliferation AT panx involvementofautophagyintantalumnanoparticleinducedosteoblastproliferation AT shaol involvementofautophagyintantalumnanoparticleinducedosteoblastproliferation
_version_	1718402968571609088

Involvement of autophagy in tantalum nanoparticle-induced osteoblast proliferation

Ejemplares similares