High prevalence of multiple antibiotic resistance in clinical E. coli isolates from Bangladesh and prediction of molecular resistance determinants using WGS of an XDR isolate

Abstract Multi-drug-resistance (MDR) is a severe public health concern worldwide, and its containment is more challenging in developing countries due to poor antimicrobial resistance (AMR) surveillance and irrational use of antibiotics. The current study investigated 100 clinical E. coli isolates an...

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Autores principales:	Preeti Jain, Asim Kumar Bepari, Prosengit Kumer Sen, Tanzir Rafe, Rashed Imtiaz, Maqsud Hossain, Hasan Mahmud Reza
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7b9cdaf3102445b9ae58c698f117416a
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spelling	oai:doaj.org-article:7b9cdaf3102445b9ae58c698f117416a2021-11-28T12:19:46ZHigh prevalence of multiple antibiotic resistance in clinical E. coli isolates from Bangladesh and prediction of molecular resistance determinants using WGS of an XDR isolate10.1038/s41598-021-02251-w2045-2322https://doaj.org/article/7b9cdaf3102445b9ae58c698f117416a2021-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-02251-whttps://doaj.org/toc/2045-2322Abstract Multi-drug-resistance (MDR) is a severe public health concern worldwide, and its containment is more challenging in developing countries due to poor antimicrobial resistance (AMR) surveillance and irrational use of antibiotics. The current study investigated 100 clinical E. coli isolates and revealed that 98% of them were MDR. PCR analysis using 25 selected isolates showed the predominance of metallo-β-lactamase gene bla NDM (80%) and ESBL genes bla OXA (48%) and bla CTX-M-15 (32%). The AmpC gene was detected in 68% of the isolates, while 32% was tetC positive. Notably, 34% of the isolates were resistant to carbapenem. Whole genome sequence (WGS) analysis of an extensively drug-resistant (XDR) isolate (L16) revealed the presence of the notorious sequence type 131 responsible for multi-drug-resistant infections, multiple antibiotic resistance genes (ARGs), virulence genes, and mobile genetic elements that pose risks to environmental transmission. Our results indicate that MDR is alarmingly increasing in Bangladesh that critically limits the treatment option against infections and contributes to further aggravation to the prevailing situation of MDR worldwide. The findings of this study will be valuable in designing sustainable strategies to contain MDR in the region.Preeti JainAsim Kumar BepariProsengit Kumer SenTanzir RafeRashed ImtiazMaqsud HossainHasan Mahmud RezaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Preeti Jain Asim Kumar Bepari Prosengit Kumer Sen Tanzir Rafe Rashed Imtiaz Maqsud Hossain Hasan Mahmud Reza High prevalence of multiple antibiotic resistance in clinical E. coli isolates from Bangladesh and prediction of molecular resistance determinants using WGS of an XDR isolate
description	Abstract Multi-drug-resistance (MDR) is a severe public health concern worldwide, and its containment is more challenging in developing countries due to poor antimicrobial resistance (AMR) surveillance and irrational use of antibiotics. The current study investigated 100 clinical E. coli isolates and revealed that 98% of them were MDR. PCR analysis using 25 selected isolates showed the predominance of metallo-β-lactamase gene bla NDM (80%) and ESBL genes bla OXA (48%) and bla CTX-M-15 (32%). The AmpC gene was detected in 68% of the isolates, while 32% was tetC positive. Notably, 34% of the isolates were resistant to carbapenem. Whole genome sequence (WGS) analysis of an extensively drug-resistant (XDR) isolate (L16) revealed the presence of the notorious sequence type 131 responsible for multi-drug-resistant infections, multiple antibiotic resistance genes (ARGs), virulence genes, and mobile genetic elements that pose risks to environmental transmission. Our results indicate that MDR is alarmingly increasing in Bangladesh that critically limits the treatment option against infections and contributes to further aggravation to the prevailing situation of MDR worldwide. The findings of this study will be valuable in designing sustainable strategies to contain MDR in the region.
format	article
author	Preeti Jain Asim Kumar Bepari Prosengit Kumer Sen Tanzir Rafe Rashed Imtiaz Maqsud Hossain Hasan Mahmud Reza
author_facet	Preeti Jain Asim Kumar Bepari Prosengit Kumer Sen Tanzir Rafe Rashed Imtiaz Maqsud Hossain Hasan Mahmud Reza
author_sort	Preeti Jain
title	High prevalence of multiple antibiotic resistance in clinical E. coli isolates from Bangladesh and prediction of molecular resistance determinants using WGS of an XDR isolate
title_short	High prevalence of multiple antibiotic resistance in clinical E. coli isolates from Bangladesh and prediction of molecular resistance determinants using WGS of an XDR isolate
title_full	High prevalence of multiple antibiotic resistance in clinical E. coli isolates from Bangladesh and prediction of molecular resistance determinants using WGS of an XDR isolate
title_fullStr	High prevalence of multiple antibiotic resistance in clinical E. coli isolates from Bangladesh and prediction of molecular resistance determinants using WGS of an XDR isolate
title_full_unstemmed	High prevalence of multiple antibiotic resistance in clinical E. coli isolates from Bangladesh and prediction of molecular resistance determinants using WGS of an XDR isolate
title_sort	high prevalence of multiple antibiotic resistance in clinical e. coli isolates from bangladesh and prediction of molecular resistance determinants using wgs of an xdr isolate
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/7b9cdaf3102445b9ae58c698f117416a
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High prevalence of multiple antibiotic resistance in clinical E. coli isolates from Bangladesh and prediction of molecular resistance determinants using WGS of an XDR isolate

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