(1)H NMR-based metabolite profiling of planktonic and biofilm cells in Acinetobacter baumannii 1656-2.

Acinetobacter baumannii is an aerobic and gram-negative pathogenic bacterium that is resistant to most antibiotics. Recently, A. baumannii 1656-2 exhibited the ability to form biofilms under clinical conditions. In this study, global metabolite profiling of both planktonic and biofilm forms of A. ba...

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Autores principales:	Jinki Yeom, Ji-Hyun Shin, Ji-Young Yang, Jungmin Kim, Geum-Sook Hwang
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7bb265f02d9448249518bec99cfa4c43
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spelling	oai:doaj.org-article:7bb265f02d9448249518bec99cfa4c432021-11-18T07:54:41Z(1)H NMR-based metabolite profiling of planktonic and biofilm cells in Acinetobacter baumannii 1656-2.1932-620310.1371/journal.pone.0057730https://doaj.org/article/7bb265f02d9448249518bec99cfa4c432013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23483923/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Acinetobacter baumannii is an aerobic and gram-negative pathogenic bacterium that is resistant to most antibiotics. Recently, A. baumannii 1656-2 exhibited the ability to form biofilms under clinical conditions. In this study, global metabolite profiling of both planktonic and biofilm forms of A. baumannii 1656-2 was performed using high-resolution nuclear magnetic resonance (NMR) spectroscopy and multivariate statistical analysis to investigate the metabolic patterns leading to biofilm formation. Principal components analysis (PCA) and orthogonal partial least-squares discriminant analysis (OPLS-DA) score plots showed a distinct separation between planktonic and biofilm cells. Metabolites including acetates, pyruvate, succinate, UDP-glucose, AMP, glutamate, and lysine were increasingly involved in the energy metabolism of biofilm formation. In particular, the ratio of N-acetyl-D-glucosamine (GlcNAc) to D-glucosamine (GlcNH2) was significantly higher during biofilm formation than under the planktonic condition. This study demonstrates that NMR-based global metabolite profiling of bacterial cells can provide valuable insight into the metabolic changes in multidrug resistant and biofilm-forming bacteria such as A. baumannii 1656-2.Jinki YeomJi-Hyun ShinJi-Young YangJungmin KimGeum-Sook HwangPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 3, p e57730 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jinki Yeom Ji-Hyun Shin Ji-Young Yang Jungmin Kim Geum-Sook Hwang (1)H NMR-based metabolite profiling of planktonic and biofilm cells in Acinetobacter baumannii 1656-2.
description	Acinetobacter baumannii is an aerobic and gram-negative pathogenic bacterium that is resistant to most antibiotics. Recently, A. baumannii 1656-2 exhibited the ability to form biofilms under clinical conditions. In this study, global metabolite profiling of both planktonic and biofilm forms of A. baumannii 1656-2 was performed using high-resolution nuclear magnetic resonance (NMR) spectroscopy and multivariate statistical analysis to investigate the metabolic patterns leading to biofilm formation. Principal components analysis (PCA) and orthogonal partial least-squares discriminant analysis (OPLS-DA) score plots showed a distinct separation between planktonic and biofilm cells. Metabolites including acetates, pyruvate, succinate, UDP-glucose, AMP, glutamate, and lysine were increasingly involved in the energy metabolism of biofilm formation. In particular, the ratio of N-acetyl-D-glucosamine (GlcNAc) to D-glucosamine (GlcNH2) was significantly higher during biofilm formation than under the planktonic condition. This study demonstrates that NMR-based global metabolite profiling of bacterial cells can provide valuable insight into the metabolic changes in multidrug resistant and biofilm-forming bacteria such as A. baumannii 1656-2.
format	article
author	Jinki Yeom Ji-Hyun Shin Ji-Young Yang Jungmin Kim Geum-Sook Hwang
author_facet	Jinki Yeom Ji-Hyun Shin Ji-Young Yang Jungmin Kim Geum-Sook Hwang
author_sort	Jinki Yeom
title	(1)H NMR-based metabolite profiling of planktonic and biofilm cells in Acinetobacter baumannii 1656-2.
title_short	(1)H NMR-based metabolite profiling of planktonic and biofilm cells in Acinetobacter baumannii 1656-2.
title_full	(1)H NMR-based metabolite profiling of planktonic and biofilm cells in Acinetobacter baumannii 1656-2.
title_fullStr	(1)H NMR-based metabolite profiling of planktonic and biofilm cells in Acinetobacter baumannii 1656-2.
title_full_unstemmed	(1)H NMR-based metabolite profiling of planktonic and biofilm cells in Acinetobacter baumannii 1656-2.
title_sort	(1)h nmr-based metabolite profiling of planktonic and biofilm cells in acinetobacter baumannii 1656-2.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/7bb265f02d9448249518bec99cfa4c43
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(1)H NMR-based metabolite profiling of planktonic and biofilm cells in Acinetobacter baumannii 1656-2.

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