Native top-down mass spectrometry provides insights into the copper centers of membrane-bound methane monooxygenase

The activity of the membrane-bound enzyme pMMO depends on copper but the location of the copper centers is still under debate. Here, the authors reconstitute pMMO in nanodiscs and use native top-down MS to localize its copper centers, providing insights into which sites are essential for activity.

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Autores principales:	Soo Y. Ro, Luis F. Schachner, Christopher W. Koo, Rahul Purohit, Jonathan P. Remis, Grace E. Kenney, Brandon W. Liauw, Paul M. Thomas, Steven M. Patrie, Neil L. Kelleher, Amy C. Rosenzweig
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/7bb55c7a2850428c843bfecc0230d63e
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spelling	oai:doaj.org-article:7bb55c7a2850428c843bfecc0230d63e2021-12-02T14:38:43ZNative top-down mass spectrometry provides insights into the copper centers of membrane-bound methane monooxygenase10.1038/s41467-019-10590-62041-1723https://doaj.org/article/7bb55c7a2850428c843bfecc0230d63e2019-06-01T00:00:00Zhttps://doi.org/10.1038/s41467-019-10590-6https://doaj.org/toc/2041-1723The activity of the membrane-bound enzyme pMMO depends on copper but the location of the copper centers is still under debate. Here, the authors reconstitute pMMO in nanodiscs and use native top-down MS to localize its copper centers, providing insights into which sites are essential for activity.Soo Y. RoLuis F. SchachnerChristopher W. KooRahul PurohitJonathan P. RemisGrace E. KenneyBrandon W. LiauwPaul M. ThomasSteven M. PatrieNeil L. KelleherAmy C. RosenzweigNature PortfolioarticleScienceQENNature Communications, Vol 10, Iss 1, Pp 1-12 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Soo Y. Ro Luis F. Schachner Christopher W. Koo Rahul Purohit Jonathan P. Remis Grace E. Kenney Brandon W. Liauw Paul M. Thomas Steven M. Patrie Neil L. Kelleher Amy C. Rosenzweig Native top-down mass spectrometry provides insights into the copper centers of membrane-bound methane monooxygenase
description	The activity of the membrane-bound enzyme pMMO depends on copper but the location of the copper centers is still under debate. Here, the authors reconstitute pMMO in nanodiscs and use native top-down MS to localize its copper centers, providing insights into which sites are essential for activity.
format	article
author	Soo Y. Ro Luis F. Schachner Christopher W. Koo Rahul Purohit Jonathan P. Remis Grace E. Kenney Brandon W. Liauw Paul M. Thomas Steven M. Patrie Neil L. Kelleher Amy C. Rosenzweig
author_facet	Soo Y. Ro Luis F. Schachner Christopher W. Koo Rahul Purohit Jonathan P. Remis Grace E. Kenney Brandon W. Liauw Paul M. Thomas Steven M. Patrie Neil L. Kelleher Amy C. Rosenzweig
author_sort	Soo Y. Ro
title	Native top-down mass spectrometry provides insights into the copper centers of membrane-bound methane monooxygenase
title_short	Native top-down mass spectrometry provides insights into the copper centers of membrane-bound methane monooxygenase
title_full	Native top-down mass spectrometry provides insights into the copper centers of membrane-bound methane monooxygenase
title_fullStr	Native top-down mass spectrometry provides insights into the copper centers of membrane-bound methane monooxygenase
title_full_unstemmed	Native top-down mass spectrometry provides insights into the copper centers of membrane-bound methane monooxygenase
title_sort	native top-down mass spectrometry provides insights into the copper centers of membrane-bound methane monooxygenase
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/7bb55c7a2850428c843bfecc0230d63e
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Native top-down mass spectrometry provides insights into the copper centers of membrane-bound methane monooxygenase

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