Thermo-regulation of genes mediating motility and plant interactions in Pseudomonas syringae.

Pseudomonas syringae is an important phyllosphere colonist that utilizes flagellum-mediated motility both as a means to explore leaf surfaces, as well as to invade into leaf interiors, where it survives as a pathogen. We found that multiple forms of flagellum-mediated motility are thermo-suppressed,...

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Autores principales:	Kevin L Hockett, Adrien Y Burch, Steven E Lindow
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7bb6ee172028442b83008005a56e1271
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spelling	oai:doaj.org-article:7bb6ee172028442b83008005a56e12712021-11-18T07:52:46ZThermo-regulation of genes mediating motility and plant interactions in Pseudomonas syringae.1932-620310.1371/journal.pone.0059850https://doaj.org/article/7bb6ee172028442b83008005a56e12712013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23527276/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Pseudomonas syringae is an important phyllosphere colonist that utilizes flagellum-mediated motility both as a means to explore leaf surfaces, as well as to invade into leaf interiors, where it survives as a pathogen. We found that multiple forms of flagellum-mediated motility are thermo-suppressed, including swarming and swimming motility. Suppression of swarming motility occurs between 28° and 30 °C, which coincides with the optimal growth temperature of P. syringae. Both fliC (encoding flagellin) and syfA (encoding a non-ribosomal peptide synthetase involved in syringafactin biosynthesis) were suppressed with increasing temperature. RNA-seq revealed 1440 genes of the P. syringae genome are temperature sensitive in expression. Genes involved in polysaccharide synthesis and regulation, phage and IS elements, type VI secretion, chemosensing and chemotaxis, translation, flagellar synthesis and motility, and phytotoxin synthesis and transport were generally repressed at 30 °C, while genes involved in transcriptional regulation, quaternary ammonium compound metabolism and transport, chaperone/heat shock proteins, and hypothetical genes were generally induced at 30 °C. Deletion of flgM, a key regulator in the transition from class III to class IV gene expression, led to elevated and constitutive expression of fliC regardless of temperature, but did not affect thermo-regulation of syfA. This work highlights the importance of temperature in the biology of P. syringae, as many genes encoding traits important for plant-microbe interactions were thermo-regulated.Kevin L HockettAdrien Y BurchSteven E LindowPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 3, p e59850 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Kevin L Hockett Adrien Y Burch Steven E Lindow Thermo-regulation of genes mediating motility and plant interactions in Pseudomonas syringae.
description	Pseudomonas syringae is an important phyllosphere colonist that utilizes flagellum-mediated motility both as a means to explore leaf surfaces, as well as to invade into leaf interiors, where it survives as a pathogen. We found that multiple forms of flagellum-mediated motility are thermo-suppressed, including swarming and swimming motility. Suppression of swarming motility occurs between 28° and 30 °C, which coincides with the optimal growth temperature of P. syringae. Both fliC (encoding flagellin) and syfA (encoding a non-ribosomal peptide synthetase involved in syringafactin biosynthesis) were suppressed with increasing temperature. RNA-seq revealed 1440 genes of the P. syringae genome are temperature sensitive in expression. Genes involved in polysaccharide synthesis and regulation, phage and IS elements, type VI secretion, chemosensing and chemotaxis, translation, flagellar synthesis and motility, and phytotoxin synthesis and transport were generally repressed at 30 °C, while genes involved in transcriptional regulation, quaternary ammonium compound metabolism and transport, chaperone/heat shock proteins, and hypothetical genes were generally induced at 30 °C. Deletion of flgM, a key regulator in the transition from class III to class IV gene expression, led to elevated and constitutive expression of fliC regardless of temperature, but did not affect thermo-regulation of syfA. This work highlights the importance of temperature in the biology of P. syringae, as many genes encoding traits important for plant-microbe interactions were thermo-regulated.
format	article
author	Kevin L Hockett Adrien Y Burch Steven E Lindow
author_facet	Kevin L Hockett Adrien Y Burch Steven E Lindow
author_sort	Kevin L Hockett
title	Thermo-regulation of genes mediating motility and plant interactions in Pseudomonas syringae.
title_short	Thermo-regulation of genes mediating motility and plant interactions in Pseudomonas syringae.
title_full	Thermo-regulation of genes mediating motility and plant interactions in Pseudomonas syringae.
title_fullStr	Thermo-regulation of genes mediating motility and plant interactions in Pseudomonas syringae.
title_full_unstemmed	Thermo-regulation of genes mediating motility and plant interactions in Pseudomonas syringae.
title_sort	thermo-regulation of genes mediating motility and plant interactions in pseudomonas syringae.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/7bb6ee172028442b83008005a56e1271
work_keys_str_mv	AT kevinlhockett thermoregulationofgenesmediatingmotilityandplantinteractionsinpseudomonassyringae AT adrienyburch thermoregulationofgenesmediatingmotilityandplantinteractionsinpseudomonassyringae AT stevenelindow thermoregulationofgenesmediatingmotilityandplantinteractionsinpseudomonassyringae
_version_	1718422816867483648

Thermo-regulation of genes mediating motility and plant interactions in Pseudomonas syringae.

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