Breast cancer metastasis suppressor 1 regulates hepatocellular carcinoma cell apoptosis via suppressing osteopontin expression.

Breast cancer metastasis suppressor 1 regulates hepatocellular carcinoma cell apoptosis via suppressing osteopontin expression.

Breast cancer metastasis suppressor 1 (BRMS1) was originally identified as an active metastasis suppressor in human breast cancer. Loss of BRMS1 expression correlates with tumor progression, and BRMS1 suppresses several steps required for tumor metastasis. However, the role of BRMS1 in hepatocellula...

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Autores principales: Yanhua Wu, Wenjun Jiang, Yingming Wang, Jun Wu, Hexige Saiyin, Xiaojing Qiao, Xinyu Mei, Bin Guo, Xiao Fang, Lu Zhang, Huiling Lou, Chaoqun Wu, Shouyi Qiao
Formato: article
Lenguaje:EN
Publicado: Public Library of Science (PLoS) 2012
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Medicine
R
Science
Q
Acceso en línea:https://doaj.org/article/7bb7db42ff8544d9981313eba6c95b63
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spelling oai:doaj.org-article:7bb7db42ff8544d9981313eba6c95b632021-11-18T07:08:07ZBreast cancer metastasis suppressor 1 regulates hepatocellular carcinoma cell apoptosis via suppressing osteopontin expression.1932-620310.1371/journal.pone.0042976https://doaj.org/article/7bb7db42ff8544d9981313eba6c95b632012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22927944/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Breast cancer metastasis suppressor 1 (BRMS1) was originally identified as an active metastasis suppressor in human breast cancer. Loss of BRMS1 expression correlates with tumor progression, and BRMS1 suppresses several steps required for tumor metastasis. However, the role of BRMS1 in hepatocellular carcinoma (HCC) remains elusive. In this study, we found that the expression level of BRMS1 was significantly down-regulated in HCC tissues. Expression of BRMS1 in SK-Hep1 cells did not affect cell growth under normal culture conditions, but sensitized cells to apoptosis induced by serum deprivation or anoikis. Consistently, knockdown of endogenous BRMS1 expression in Hep3B cells suppressed cell apoptosis. We identified that BRMS1 suppresses osteopontin (OPN) expression in HCC cells and that there is a negative correlation between BRMS1 and OPN mRNA expression in HCC tissues. Moreover, knockdown of endogenous OPN expression reversed the anti-apoptosis effect achieved by knockdown of BRMS1. Taken together, our results show that BRMS1 sensitizes HCC cells to apoptosis through suppressing OPN expression, suggesting a potential role of BRMS1 in regulating HCC apoptosis and metastasis.Yanhua WuWenjun JiangYingming WangJun WuHexige SaiyinXiaojing QiaoXinyu MeiBin GuoXiao FangLu ZhangHuiling LouChaoqun WuShouyi QiaoPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 8, p e42976 (2012)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Yanhua Wu
Wenjun Jiang
Yingming Wang
Jun Wu
Hexige Saiyin
Xiaojing Qiao
Xinyu Mei
Bin Guo
Xiao Fang
Lu Zhang
Huiling Lou
Chaoqun Wu
Shouyi Qiao
Breast cancer metastasis suppressor 1 regulates hepatocellular carcinoma cell apoptosis via suppressing osteopontin expression.
description Breast cancer metastasis suppressor 1 (BRMS1) was originally identified as an active metastasis suppressor in human breast cancer. Loss of BRMS1 expression correlates with tumor progression, and BRMS1 suppresses several steps required for tumor metastasis. However, the role of BRMS1 in hepatocellular carcinoma (HCC) remains elusive. In this study, we found that the expression level of BRMS1 was significantly down-regulated in HCC tissues. Expression of BRMS1 in SK-Hep1 cells did not affect cell growth under normal culture conditions, but sensitized cells to apoptosis induced by serum deprivation or anoikis. Consistently, knockdown of endogenous BRMS1 expression in Hep3B cells suppressed cell apoptosis. We identified that BRMS1 suppresses osteopontin (OPN) expression in HCC cells and that there is a negative correlation between BRMS1 and OPN mRNA expression in HCC tissues. Moreover, knockdown of endogenous OPN expression reversed the anti-apoptosis effect achieved by knockdown of BRMS1. Taken together, our results show that BRMS1 sensitizes HCC cells to apoptosis through suppressing OPN expression, suggesting a potential role of BRMS1 in regulating HCC apoptosis and metastasis.
format article
author Yanhua Wu
Wenjun Jiang
Yingming Wang
Jun Wu
Hexige Saiyin
Xiaojing Qiao
Xinyu Mei
Bin Guo
Xiao Fang
Lu Zhang
Huiling Lou
Chaoqun Wu
Shouyi Qiao
author_facet Yanhua Wu
Wenjun Jiang
Yingming Wang
Jun Wu
Hexige Saiyin
Xiaojing Qiao
Xinyu Mei
Bin Guo
Xiao Fang
Lu Zhang
Huiling Lou
Chaoqun Wu
Shouyi Qiao
author_sort Yanhua Wu
title Breast cancer metastasis suppressor 1 regulates hepatocellular carcinoma cell apoptosis via suppressing osteopontin expression.
title_short Breast cancer metastasis suppressor 1 regulates hepatocellular carcinoma cell apoptosis via suppressing osteopontin expression.
title_full Breast cancer metastasis suppressor 1 regulates hepatocellular carcinoma cell apoptosis via suppressing osteopontin expression.
title_fullStr Breast cancer metastasis suppressor 1 regulates hepatocellular carcinoma cell apoptosis via suppressing osteopontin expression.
title_full_unstemmed Breast cancer metastasis suppressor 1 regulates hepatocellular carcinoma cell apoptosis via suppressing osteopontin expression.
title_sort breast cancer metastasis suppressor 1 regulates hepatocellular carcinoma cell apoptosis via suppressing osteopontin expression.
publisher Public Library of Science (PLoS)
publishDate 2012
url https://doaj.org/article/7bb7db42ff8544d9981313eba6c95b63
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