Personalized medicine for reconstruction of critical-size bone defects – a translational approach with customizable vascularized bone tissue
Abstract Tissue engineering principles allow the generation of functional tissues for biomedical applications. Reconstruction of large-scale bone defects with tissue-engineered bone has still not entered the clinical routine. In the present study, a bone substitute in combination with mesenchymal st...
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2021
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oai:doaj.org-article:7bc513d32fce404882b1dcac553659de2021-12-02T18:01:18ZPersonalized medicine for reconstruction of critical-size bone defects – a translational approach with customizable vascularized bone tissue10.1038/s41536-021-00158-82057-3995https://doaj.org/article/7bc513d32fce404882b1dcac553659de2021-08-01T00:00:00Zhttps://doi.org/10.1038/s41536-021-00158-8https://doaj.org/toc/2057-3995Abstract Tissue engineering principles allow the generation of functional tissues for biomedical applications. Reconstruction of large-scale bone defects with tissue-engineered bone has still not entered the clinical routine. In the present study, a bone substitute in combination with mesenchymal stem cells (MSC) and endothelial progenitor cells (EPC) with or without growth factors BMP-2 and VEGF-A was prevascularized by an arteriovenous (AV) loop and transplanted into a critical-size tibia defect in the sheep model. With 3D imaging and immunohistochemistry, we could show that this approach is a feasible and simple alternative to the current clinical therapeutic option. This study serves as proof of concept for using large-scale transplantable, vascularized, and customizable bone, generated in a living organism for the reconstruction of load-bearing bone defects, individually tailored to the patient’s needs. With this approach in personalized medicine for the reconstruction of critical-size bone defects, regeneration of parts of the human body will become possible in the near future.Annika Kengelbach-WeigandCarolina ThielenTobias BäuerleRebekka GötzlThomas GerberCarolin KörnerJustus P. BeierRaymund E. HorchAnja M. BoosNature PortfolioarticleMedicineRENnpj Regenerative Medicine, Vol 6, Iss 1, Pp 1-13 (2021) |
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Medicine R Annika Kengelbach-Weigand Carolina Thielen Tobias Bäuerle Rebekka Götzl Thomas Gerber Carolin Körner Justus P. Beier Raymund E. Horch Anja M. Boos Personalized medicine for reconstruction of critical-size bone defects – a translational approach with customizable vascularized bone tissue |
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Abstract Tissue engineering principles allow the generation of functional tissues for biomedical applications. Reconstruction of large-scale bone defects with tissue-engineered bone has still not entered the clinical routine. In the present study, a bone substitute in combination with mesenchymal stem cells (MSC) and endothelial progenitor cells (EPC) with or without growth factors BMP-2 and VEGF-A was prevascularized by an arteriovenous (AV) loop and transplanted into a critical-size tibia defect in the sheep model. With 3D imaging and immunohistochemistry, we could show that this approach is a feasible and simple alternative to the current clinical therapeutic option. This study serves as proof of concept for using large-scale transplantable, vascularized, and customizable bone, generated in a living organism for the reconstruction of load-bearing bone defects, individually tailored to the patient’s needs. With this approach in personalized medicine for the reconstruction of critical-size bone defects, regeneration of parts of the human body will become possible in the near future. |
format |
article |
author |
Annika Kengelbach-Weigand Carolina Thielen Tobias Bäuerle Rebekka Götzl Thomas Gerber Carolin Körner Justus P. Beier Raymund E. Horch Anja M. Boos |
author_facet |
Annika Kengelbach-Weigand Carolina Thielen Tobias Bäuerle Rebekka Götzl Thomas Gerber Carolin Körner Justus P. Beier Raymund E. Horch Anja M. Boos |
author_sort |
Annika Kengelbach-Weigand |
title |
Personalized medicine for reconstruction of critical-size bone defects – a translational approach with customizable vascularized bone tissue |
title_short |
Personalized medicine for reconstruction of critical-size bone defects – a translational approach with customizable vascularized bone tissue |
title_full |
Personalized medicine for reconstruction of critical-size bone defects – a translational approach with customizable vascularized bone tissue |
title_fullStr |
Personalized medicine for reconstruction of critical-size bone defects – a translational approach with customizable vascularized bone tissue |
title_full_unstemmed |
Personalized medicine for reconstruction of critical-size bone defects – a translational approach with customizable vascularized bone tissue |
title_sort |
personalized medicine for reconstruction of critical-size bone defects – a translational approach with customizable vascularized bone tissue |
publisher |
Nature Portfolio |
publishDate |
2021 |
url |
https://doaj.org/article/7bc513d32fce404882b1dcac553659de |
work_keys_str_mv |
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