Personalized medicine for reconstruction of critical-size bone defects – a translational approach with customizable vascularized bone tissue

Abstract Tissue engineering principles allow the generation of functional tissues for biomedical applications. Reconstruction of large-scale bone defects with tissue-engineered bone has still not entered the clinical routine. In the present study, a bone substitute in combination with mesenchymal st...

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Autores principales:	Annika Kengelbach-Weigand, Carolina Thielen, Tobias Bäuerle, Rebekka Götzl, Thomas Gerber, Carolin Körner, Justus P. Beier, Raymund E. Horch, Anja M. Boos
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R
Acceso en línea:	https://doaj.org/article/7bc513d32fce404882b1dcac553659de
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spelling	oai:doaj.org-article:7bc513d32fce404882b1dcac553659de2021-12-02T18:01:18ZPersonalized medicine for reconstruction of critical-size bone defects – a translational approach with customizable vascularized bone tissue10.1038/s41536-021-00158-82057-3995https://doaj.org/article/7bc513d32fce404882b1dcac553659de2021-08-01T00:00:00Zhttps://doi.org/10.1038/s41536-021-00158-8https://doaj.org/toc/2057-3995Abstract Tissue engineering principles allow the generation of functional tissues for biomedical applications. Reconstruction of large-scale bone defects with tissue-engineered bone has still not entered the clinical routine. In the present study, a bone substitute in combination with mesenchymal stem cells (MSC) and endothelial progenitor cells (EPC) with or without growth factors BMP-2 and VEGF-A was prevascularized by an arteriovenous (AV) loop and transplanted into a critical-size tibia defect in the sheep model. With 3D imaging and immunohistochemistry, we could show that this approach is a feasible and simple alternative to the current clinical therapeutic option. This study serves as proof of concept for using large-scale transplantable, vascularized, and customizable bone, generated in a living organism for the reconstruction of load-bearing bone defects, individually tailored to the patient’s needs. With this approach in personalized medicine for the reconstruction of critical-size bone defects, regeneration of parts of the human body will become possible in the near future.Annika Kengelbach-WeigandCarolina ThielenTobias BäuerleRebekka GötzlThomas GerberCarolin KörnerJustus P. BeierRaymund E. HorchAnja M. BoosNature PortfolioarticleMedicineRENnpj Regenerative Medicine, Vol 6, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R
spellingShingle	Medicine R Annika Kengelbach-Weigand Carolina Thielen Tobias Bäuerle Rebekka Götzl Thomas Gerber Carolin Körner Justus P. Beier Raymund E. Horch Anja M. Boos Personalized medicine for reconstruction of critical-size bone defects – a translational approach with customizable vascularized bone tissue
description	Abstract Tissue engineering principles allow the generation of functional tissues for biomedical applications. Reconstruction of large-scale bone defects with tissue-engineered bone has still not entered the clinical routine. In the present study, a bone substitute in combination with mesenchymal stem cells (MSC) and endothelial progenitor cells (EPC) with or without growth factors BMP-2 and VEGF-A was prevascularized by an arteriovenous (AV) loop and transplanted into a critical-size tibia defect in the sheep model. With 3D imaging and immunohistochemistry, we could show that this approach is a feasible and simple alternative to the current clinical therapeutic option. This study serves as proof of concept for using large-scale transplantable, vascularized, and customizable bone, generated in a living organism for the reconstruction of load-bearing bone defects, individually tailored to the patient’s needs. With this approach in personalized medicine for the reconstruction of critical-size bone defects, regeneration of parts of the human body will become possible in the near future.
format	article
author	Annika Kengelbach-Weigand Carolina Thielen Tobias Bäuerle Rebekka Götzl Thomas Gerber Carolin Körner Justus P. Beier Raymund E. Horch Anja M. Boos
author_facet	Annika Kengelbach-Weigand Carolina Thielen Tobias Bäuerle Rebekka Götzl Thomas Gerber Carolin Körner Justus P. Beier Raymund E. Horch Anja M. Boos
author_sort	Annika Kengelbach-Weigand
title	Personalized medicine for reconstruction of critical-size bone defects – a translational approach with customizable vascularized bone tissue
title_short	Personalized medicine for reconstruction of critical-size bone defects – a translational approach with customizable vascularized bone tissue
title_full	Personalized medicine for reconstruction of critical-size bone defects – a translational approach with customizable vascularized bone tissue
title_fullStr	Personalized medicine for reconstruction of critical-size bone defects – a translational approach with customizable vascularized bone tissue
title_full_unstemmed	Personalized medicine for reconstruction of critical-size bone defects – a translational approach with customizable vascularized bone tissue
title_sort	personalized medicine for reconstruction of critical-size bone defects – a translational approach with customizable vascularized bone tissue
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/7bc513d32fce404882b1dcac553659de
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Personalized medicine for reconstruction of critical-size bone defects – a translational approach with customizable vascularized bone tissue

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