Personalized medicine for reconstruction of critical-size bone defects – a translational approach with customizable vascularized bone tissue

Abstract Tissue engineering principles allow the generation of functional tissues for biomedical applications. Reconstruction of large-scale bone defects with tissue-engineered bone has still not entered the clinical routine. In the present study, a bone substitute in combination with mesenchymal st...

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Autores principales: Annika Kengelbach-Weigand, Carolina Thielen, Tobias Bäuerle, Rebekka Götzl, Thomas Gerber, Carolin Körner, Justus P. Beier, Raymund E. Horch, Anja M. Boos
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Publicado: Nature Portfolio 2021
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Acceso en línea:https://doaj.org/article/7bc513d32fce404882b1dcac553659de
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spelling oai:doaj.org-article:7bc513d32fce404882b1dcac553659de2021-12-02T18:01:18ZPersonalized medicine for reconstruction of critical-size bone defects – a translational approach with customizable vascularized bone tissue10.1038/s41536-021-00158-82057-3995https://doaj.org/article/7bc513d32fce404882b1dcac553659de2021-08-01T00:00:00Zhttps://doi.org/10.1038/s41536-021-00158-8https://doaj.org/toc/2057-3995Abstract Tissue engineering principles allow the generation of functional tissues for biomedical applications. Reconstruction of large-scale bone defects with tissue-engineered bone has still not entered the clinical routine. In the present study, a bone substitute in combination with mesenchymal stem cells (MSC) and endothelial progenitor cells (EPC) with or without growth factors BMP-2 and VEGF-A was prevascularized by an arteriovenous (AV) loop and transplanted into a critical-size tibia defect in the sheep model. With 3D imaging and immunohistochemistry, we could show that this approach is a feasible and simple alternative to the current clinical therapeutic option. This study serves as proof of concept for using large-scale transplantable, vascularized, and customizable bone, generated in a living organism for the reconstruction of load-bearing bone defects, individually tailored to the patient’s needs. With this approach in personalized medicine for the reconstruction of critical-size bone defects, regeneration of parts of the human body will become possible in the near future.Annika Kengelbach-WeigandCarolina ThielenTobias BäuerleRebekka GötzlThomas GerberCarolin KörnerJustus P. BeierRaymund E. HorchAnja M. BoosNature PortfolioarticleMedicineRENnpj Regenerative Medicine, Vol 6, Iss 1, Pp 1-13 (2021)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
spellingShingle Medicine
R
Annika Kengelbach-Weigand
Carolina Thielen
Tobias Bäuerle
Rebekka Götzl
Thomas Gerber
Carolin Körner
Justus P. Beier
Raymund E. Horch
Anja M. Boos
Personalized medicine for reconstruction of critical-size bone defects – a translational approach with customizable vascularized bone tissue
description Abstract Tissue engineering principles allow the generation of functional tissues for biomedical applications. Reconstruction of large-scale bone defects with tissue-engineered bone has still not entered the clinical routine. In the present study, a bone substitute in combination with mesenchymal stem cells (MSC) and endothelial progenitor cells (EPC) with or without growth factors BMP-2 and VEGF-A was prevascularized by an arteriovenous (AV) loop and transplanted into a critical-size tibia defect in the sheep model. With 3D imaging and immunohistochemistry, we could show that this approach is a feasible and simple alternative to the current clinical therapeutic option. This study serves as proof of concept for using large-scale transplantable, vascularized, and customizable bone, generated in a living organism for the reconstruction of load-bearing bone defects, individually tailored to the patient’s needs. With this approach in personalized medicine for the reconstruction of critical-size bone defects, regeneration of parts of the human body will become possible in the near future.
format article
author Annika Kengelbach-Weigand
Carolina Thielen
Tobias Bäuerle
Rebekka Götzl
Thomas Gerber
Carolin Körner
Justus P. Beier
Raymund E. Horch
Anja M. Boos
author_facet Annika Kengelbach-Weigand
Carolina Thielen
Tobias Bäuerle
Rebekka Götzl
Thomas Gerber
Carolin Körner
Justus P. Beier
Raymund E. Horch
Anja M. Boos
author_sort Annika Kengelbach-Weigand
title Personalized medicine for reconstruction of critical-size bone defects – a translational approach with customizable vascularized bone tissue
title_short Personalized medicine for reconstruction of critical-size bone defects – a translational approach with customizable vascularized bone tissue
title_full Personalized medicine for reconstruction of critical-size bone defects – a translational approach with customizable vascularized bone tissue
title_fullStr Personalized medicine for reconstruction of critical-size bone defects – a translational approach with customizable vascularized bone tissue
title_full_unstemmed Personalized medicine for reconstruction of critical-size bone defects – a translational approach with customizable vascularized bone tissue
title_sort personalized medicine for reconstruction of critical-size bone defects – a translational approach with customizable vascularized bone tissue
publisher Nature Portfolio
publishDate 2021
url https://doaj.org/article/7bc513d32fce404882b1dcac553659de
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