CSF-1 regulates the function of monocytes in Crohn’s disease patients in remission

Abstract During the flare-ups of Crohn’s disease (CD) patients, circulating leukocytes actively migrate toward the inflamed sites. During the remission, the lack of symptoms does not necessarily imply immunological remission. To decipher inflammatory mechanisms still operating during CD remission, w...

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Autores principales:	Juan Camilo Nieto, Carlos Zamora, Elisabet Cantó, Esther Garcia-Planella, Jordi Gordillo, Maria Angels Ortiz, Cándido Juárez, Silvia Vidal
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7bcdde80196145a1b3509c248893f0da
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spelling	oai:doaj.org-article:7bcdde80196145a1b3509c248893f0da2021-12-02T12:32:58ZCSF-1 regulates the function of monocytes in Crohn’s disease patients in remission10.1038/s41598-017-00145-42045-2322https://doaj.org/article/7bcdde80196145a1b3509c248893f0da2017-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00145-4https://doaj.org/toc/2045-2322Abstract During the flare-ups of Crohn’s disease (CD) patients, circulating leukocytes actively migrate toward the inflamed sites. During the remission, the lack of symptoms does not necessarily imply immunological remission. To decipher inflammatory mechanisms still operating during CD remission, we compared the expression of chemokine receptors on monocytes from CD and healthy donors (HD), and how these differences could modulate monocyte maturation and cytokine production. Flow cytometry analysis showed a higher expression of CCR5 on monocytes from CD patients than those from HD after 24 h. This CCR5 upregulation was associated with the spontaneous production of CSF-1 and IL-10. The higher expression of CCR5 on CD monocytes increased their migratory pattern in response to CCL5. Signaling through CCR5/CCL5 increased CD163 and HLA-DR expression and diminished TLR4-induced TNF-α and IL-6 secretion during monocyte differentiation. When we analyzed clinical parameters, patients treated with azathioprine had the highest CSF-1 levels and CCR5 expression. Our results suggest that monocytes from CD patients in remission produced high levels of CSF-1 that upregulate CCR5 expression. Consequently, monocytes differentiated in these conditions had a characteristic phenotype and lower production of inflammatory cytokines. The treatment with azathioprine could be responsible for this anti-inflammatory profile of monocytes.Juan Camilo NietoCarlos ZamoraElisabet CantóEsther Garcia-PlanellaJordi GordilloMaria Angels OrtizCándido JuárezSilvia VidalNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Juan Camilo Nieto Carlos Zamora Elisabet Cantó Esther Garcia-Planella Jordi Gordillo Maria Angels Ortiz Cándido Juárez Silvia Vidal CSF-1 regulates the function of monocytes in Crohn’s disease patients in remission
description	Abstract During the flare-ups of Crohn’s disease (CD) patients, circulating leukocytes actively migrate toward the inflamed sites. During the remission, the lack of symptoms does not necessarily imply immunological remission. To decipher inflammatory mechanisms still operating during CD remission, we compared the expression of chemokine receptors on monocytes from CD and healthy donors (HD), and how these differences could modulate monocyte maturation and cytokine production. Flow cytometry analysis showed a higher expression of CCR5 on monocytes from CD patients than those from HD after 24 h. This CCR5 upregulation was associated with the spontaneous production of CSF-1 and IL-10. The higher expression of CCR5 on CD monocytes increased their migratory pattern in response to CCL5. Signaling through CCR5/CCL5 increased CD163 and HLA-DR expression and diminished TLR4-induced TNF-α and IL-6 secretion during monocyte differentiation. When we analyzed clinical parameters, patients treated with azathioprine had the highest CSF-1 levels and CCR5 expression. Our results suggest that monocytes from CD patients in remission produced high levels of CSF-1 that upregulate CCR5 expression. Consequently, monocytes differentiated in these conditions had a characteristic phenotype and lower production of inflammatory cytokines. The treatment with azathioprine could be responsible for this anti-inflammatory profile of monocytes.
format	article
author	Juan Camilo Nieto Carlos Zamora Elisabet Cantó Esther Garcia-Planella Jordi Gordillo Maria Angels Ortiz Cándido Juárez Silvia Vidal
author_facet	Juan Camilo Nieto Carlos Zamora Elisabet Cantó Esther Garcia-Planella Jordi Gordillo Maria Angels Ortiz Cándido Juárez Silvia Vidal
author_sort	Juan Camilo Nieto
title	CSF-1 regulates the function of monocytes in Crohn’s disease patients in remission
title_short	CSF-1 regulates the function of monocytes in Crohn’s disease patients in remission
title_full	CSF-1 regulates the function of monocytes in Crohn’s disease patients in remission
title_fullStr	CSF-1 regulates the function of monocytes in Crohn’s disease patients in remission
title_full_unstemmed	CSF-1 regulates the function of monocytes in Crohn’s disease patients in remission
title_sort	csf-1 regulates the function of monocytes in crohn’s disease patients in remission
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/7bcdde80196145a1b3509c248893f0da
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CSF-1 regulates the function of monocytes in Crohn’s disease patients in remission

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