Effect of Central Administration of Brain-Derived Neurotrophic Factor (BDNF) on Behavior and Brain Monoamine Metabolism in New Recombinant Mouse Lines Differing by 5-HT<sub>1A</sub> Receptor Functionality
Experiments were carried out on recombinant B6.CBA-D13Mit76C (B6-M76C) and B6.CBA-D13Mit76B (B6-M76B) mouse lines created by transferring a 102.73–118.83 Mbp fragment of chromosome 13, containing the 5-HT<sub>1A</sub> receptor gene, from CBA or C57BL/6 strains to a C57BL/6 genetic backgr...
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2021
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oai:doaj.org-article:7bd430983ff24579b021f2c4e64cf2462021-11-11T17:24:26ZEffect of Central Administration of Brain-Derived Neurotrophic Factor (BDNF) on Behavior and Brain Monoamine Metabolism in New Recombinant Mouse Lines Differing by 5-HT<sub>1A</sub> Receptor Functionality10.3390/ijms2221119871422-00671661-6596https://doaj.org/article/7bd430983ff24579b021f2c4e64cf2462021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/21/11987https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Experiments were carried out on recombinant B6.CBA-D13Mit76C (B6-M76C) and B6.CBA-D13Mit76B (B6-M76B) mouse lines created by transferring a 102.73–118.83 Mbp fragment of chromosome 13, containing the 5-HT<sub>1A</sub> receptor gene, from CBA or C57BL/6 strains to a C57BL/6 genetic background, correspondingly. We have recently shown different levels of 5-HT1A receptor functionality in these mouse lines. The administration of BDNF (300 ng/mouse, i.c.v.) increased the levels of exploratory activity and intermale aggression only in B6-M76B mice, without affecting depressive-like behavior in both lines. In B6-M76B mice the behavioral alterations were accompanied by a decrease in the 5-HT<sub>2A</sub> receptor functional activity and the augmentation of levels of serotonin and its main metabolite, 5-HIAA (5-hydroxyindoleacetic acid), in the midbrain. Moreover, the levels of dopamine and its main metabolites, HVA (homovanillic acid) and DOPAC (3,4-dihydroxyphenylacetic acid), were also elevated in the striatum of B6-M76B mice after BDNF treatment. In B6-M76C mice, central BDNF administration led only to a reduction in the functional activity of the 5-HT<sub>1A</sub> receptor and a rise in DOPAC levels in the midbrain. The obtained data suggest the importance of the 102.73–118.83 Mbp fragment of mouse chromosome 13, which contains the 5-HT<sub>1A</sub> receptor gene, for BDNF-induced alterations in behavior and the brain monoamine system.Darya BazovkinaVladimir NaumenkoEkaterina BazhenovaElena KondaurovaMDPI AGarticleBDNFaggression5-HT<sub>1A</sub> and 5-HT<sub>2A</sub> receptors functional activitybrain serotonin systembrain dopamine systemHPLCBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 11987, p 11987 (2021) |
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DOAJ |
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BDNF aggression 5-HT<sub>1A</sub> and 5-HT<sub>2A</sub> receptors functional activity brain serotonin system brain dopamine system HPLC Biology (General) QH301-705.5 Chemistry QD1-999 |
| spellingShingle |
BDNF aggression 5-HT<sub>1A</sub> and 5-HT<sub>2A</sub> receptors functional activity brain serotonin system brain dopamine system HPLC Biology (General) QH301-705.5 Chemistry QD1-999 Darya Bazovkina Vladimir Naumenko Ekaterina Bazhenova Elena Kondaurova Effect of Central Administration of Brain-Derived Neurotrophic Factor (BDNF) on Behavior and Brain Monoamine Metabolism in New Recombinant Mouse Lines Differing by 5-HT<sub>1A</sub> Receptor Functionality |
| description |
Experiments were carried out on recombinant B6.CBA-D13Mit76C (B6-M76C) and B6.CBA-D13Mit76B (B6-M76B) mouse lines created by transferring a 102.73–118.83 Mbp fragment of chromosome 13, containing the 5-HT<sub>1A</sub> receptor gene, from CBA or C57BL/6 strains to a C57BL/6 genetic background, correspondingly. We have recently shown different levels of 5-HT1A receptor functionality in these mouse lines. The administration of BDNF (300 ng/mouse, i.c.v.) increased the levels of exploratory activity and intermale aggression only in B6-M76B mice, without affecting depressive-like behavior in both lines. In B6-M76B mice the behavioral alterations were accompanied by a decrease in the 5-HT<sub>2A</sub> receptor functional activity and the augmentation of levels of serotonin and its main metabolite, 5-HIAA (5-hydroxyindoleacetic acid), in the midbrain. Moreover, the levels of dopamine and its main metabolites, HVA (homovanillic acid) and DOPAC (3,4-dihydroxyphenylacetic acid), were also elevated in the striatum of B6-M76B mice after BDNF treatment. In B6-M76C mice, central BDNF administration led only to a reduction in the functional activity of the 5-HT<sub>1A</sub> receptor and a rise in DOPAC levels in the midbrain. The obtained data suggest the importance of the 102.73–118.83 Mbp fragment of mouse chromosome 13, which contains the 5-HT<sub>1A</sub> receptor gene, for BDNF-induced alterations in behavior and the brain monoamine system. |
| format |
article |
| author |
Darya Bazovkina Vladimir Naumenko Ekaterina Bazhenova Elena Kondaurova |
| author_facet |
Darya Bazovkina Vladimir Naumenko Ekaterina Bazhenova Elena Kondaurova |
| author_sort |
Darya Bazovkina |
| title |
Effect of Central Administration of Brain-Derived Neurotrophic Factor (BDNF) on Behavior and Brain Monoamine Metabolism in New Recombinant Mouse Lines Differing by 5-HT<sub>1A</sub> Receptor Functionality |
| title_short |
Effect of Central Administration of Brain-Derived Neurotrophic Factor (BDNF) on Behavior and Brain Monoamine Metabolism in New Recombinant Mouse Lines Differing by 5-HT<sub>1A</sub> Receptor Functionality |
| title_full |
Effect of Central Administration of Brain-Derived Neurotrophic Factor (BDNF) on Behavior and Brain Monoamine Metabolism in New Recombinant Mouse Lines Differing by 5-HT<sub>1A</sub> Receptor Functionality |
| title_fullStr |
Effect of Central Administration of Brain-Derived Neurotrophic Factor (BDNF) on Behavior and Brain Monoamine Metabolism in New Recombinant Mouse Lines Differing by 5-HT<sub>1A</sub> Receptor Functionality |
| title_full_unstemmed |
Effect of Central Administration of Brain-Derived Neurotrophic Factor (BDNF) on Behavior and Brain Monoamine Metabolism in New Recombinant Mouse Lines Differing by 5-HT<sub>1A</sub> Receptor Functionality |
| title_sort |
effect of central administration of brain-derived neurotrophic factor (bdnf) on behavior and brain monoamine metabolism in new recombinant mouse lines differing by 5-ht<sub>1a</sub> receptor functionality |
| publisher |
MDPI AG |
| publishDate |
2021 |
| url |
https://doaj.org/article/7bd430983ff24579b021f2c4e64cf246 |
| work_keys_str_mv |
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