Full-length recombinant human SCF1-165 is more thermostable than the truncated SCF1-141 form.

Human stem cell factor initiates a diverse array of cellular responses, including hematopoiesis, cell proliferation, differentiation, migration and survival. To explore the relationship between its structure and function, we produced recombinant soluble human stem cell factor1-165 (wild type) and hu...

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Autores principales:	Yui-Ping Weng, Wen-Yen Ku, Ming-Han Wu, Ya-Li Tsai, Chi-Yu Chen, Chun-An Kuo, Lynn L H Huang
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7bd9bf896e644feeaded232c31c8a92e
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spelling	oai:doaj.org-article:7bd9bf896e644feeaded232c31c8a92e2021-11-25T06:07:08ZFull-length recombinant human SCF1-165 is more thermostable than the truncated SCF1-141 form.1932-620310.1371/journal.pone.0103251https://doaj.org/article/7bd9bf896e644feeaded232c31c8a92e2014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/25061857/?tool=EBIhttps://doaj.org/toc/1932-6203Human stem cell factor initiates a diverse array of cellular responses, including hematopoiesis, cell proliferation, differentiation, migration and survival. To explore the relationship between its structure and function, we produced recombinant soluble human stem cell factor1-165 (wild type) and human stem cell factor1-141 (C-terminal truncated) in a yeast expression system and compared their biological activities and thermal stabilities. The biological activity of the two proteins was measured as a function of TF-1 cell viability and effects on downstream signaling targets after incubation. We found that these proteins enhanced cell viability and downstream signaling to a similar extent, in a dose-dependent manner. The biological activity of recombinant human stem cell factor1-165 was significantly greater than that of recombinant human stem cell factor1-141 after heating the proteins (100 ng/mL) at 25-110°C for 10 minutes (P<0.05 for all temperatures). In addition, circular dichroism spectral analysis indicated that β-sheet structures were altered in recombinant human stem cell factor1-141 but not recombinant human stem cell factor1-165 after heating at 90°C for 15 or 30 min. Molecular modeling and limited proteolytic digestion were also used to compare the thermo stability between human stem cell factor1-165 and human stem cell factor1-141. Together, these data indicate that stem cell factor1-165 is more thermostable than stem cell factor1-141.Yui-Ping WengWen-Yen KuMing-Han WuYa-Li TsaiChi-Yu ChenChun-An KuoLynn L H HuangPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 7, p e103251 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yui-Ping Weng Wen-Yen Ku Ming-Han Wu Ya-Li Tsai Chi-Yu Chen Chun-An Kuo Lynn L H Huang Full-length recombinant human SCF1-165 is more thermostable than the truncated SCF1-141 form.
description	Human stem cell factor initiates a diverse array of cellular responses, including hematopoiesis, cell proliferation, differentiation, migration and survival. To explore the relationship between its structure and function, we produced recombinant soluble human stem cell factor1-165 (wild type) and human stem cell factor1-141 (C-terminal truncated) in a yeast expression system and compared their biological activities and thermal stabilities. The biological activity of the two proteins was measured as a function of TF-1 cell viability and effects on downstream signaling targets after incubation. We found that these proteins enhanced cell viability and downstream signaling to a similar extent, in a dose-dependent manner. The biological activity of recombinant human stem cell factor1-165 was significantly greater than that of recombinant human stem cell factor1-141 after heating the proteins (100 ng/mL) at 25-110°C for 10 minutes (P<0.05 for all temperatures). In addition, circular dichroism spectral analysis indicated that β-sheet structures were altered in recombinant human stem cell factor1-141 but not recombinant human stem cell factor1-165 after heating at 90°C for 15 or 30 min. Molecular modeling and limited proteolytic digestion were also used to compare the thermo stability between human stem cell factor1-165 and human stem cell factor1-141. Together, these data indicate that stem cell factor1-165 is more thermostable than stem cell factor1-141.
format	article
author	Yui-Ping Weng Wen-Yen Ku Ming-Han Wu Ya-Li Tsai Chi-Yu Chen Chun-An Kuo Lynn L H Huang
author_facet	Yui-Ping Weng Wen-Yen Ku Ming-Han Wu Ya-Li Tsai Chi-Yu Chen Chun-An Kuo Lynn L H Huang
author_sort	Yui-Ping Weng
title	Full-length recombinant human SCF1-165 is more thermostable than the truncated SCF1-141 form.
title_short	Full-length recombinant human SCF1-165 is more thermostable than the truncated SCF1-141 form.
title_full	Full-length recombinant human SCF1-165 is more thermostable than the truncated SCF1-141 form.
title_fullStr	Full-length recombinant human SCF1-165 is more thermostable than the truncated SCF1-141 form.
title_full_unstemmed	Full-length recombinant human SCF1-165 is more thermostable than the truncated SCF1-141 form.
title_sort	full-length recombinant human scf1-165 is more thermostable than the truncated scf1-141 form.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/7bd9bf896e644feeaded232c31c8a92e
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Full-length recombinant human SCF1-165 is more thermostable than the truncated SCF1-141 form.

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