Label-free spatio-temporal monitoring of cytosolic mass, osmolarity, and volume in living cells
Label-free, spatio-temporal imaging of cellular physiological responses is challenging. Here the authors combine digital holographic microscopy with a millifluidic chip and mathematical modelling to quantify cell volume, mass and cell uptake under changing environmental conditions.
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Nature Portfolio
2019
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oai:doaj.org-article:7beea7bfef8044bd83a66a1ec7e086bb2021-12-02T17:01:28ZLabel-free spatio-temporal monitoring of cytosolic mass, osmolarity, and volume in living cells10.1038/s41467-018-08207-52041-1723https://doaj.org/article/7beea7bfef8044bd83a66a1ec7e086bb2019-01-01T00:00:00Zhttps://doi.org/10.1038/s41467-018-08207-5https://doaj.org/toc/2041-1723Label-free, spatio-temporal imaging of cellular physiological responses is challenging. Here the authors combine digital holographic microscopy with a millifluidic chip and mathematical modelling to quantify cell volume, mass and cell uptake under changing environmental conditions.Daniel MidtvedtErik OlsénFredrik HöökGavin D. M. JeffriesNature PortfolioarticleScienceQENNature Communications, Vol 10, Iss 1, Pp 1-9 (2019) |
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Science Q |
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Science Q Daniel Midtvedt Erik Olsén Fredrik Höök Gavin D. M. Jeffries Label-free spatio-temporal monitoring of cytosolic mass, osmolarity, and volume in living cells |
description |
Label-free, spatio-temporal imaging of cellular physiological responses is challenging. Here the authors combine digital holographic microscopy with a millifluidic chip and mathematical modelling to quantify cell volume, mass and cell uptake under changing environmental conditions. |
format |
article |
author |
Daniel Midtvedt Erik Olsén Fredrik Höök Gavin D. M. Jeffries |
author_facet |
Daniel Midtvedt Erik Olsén Fredrik Höök Gavin D. M. Jeffries |
author_sort |
Daniel Midtvedt |
title |
Label-free spatio-temporal monitoring of cytosolic mass, osmolarity, and volume in living cells |
title_short |
Label-free spatio-temporal monitoring of cytosolic mass, osmolarity, and volume in living cells |
title_full |
Label-free spatio-temporal monitoring of cytosolic mass, osmolarity, and volume in living cells |
title_fullStr |
Label-free spatio-temporal monitoring of cytosolic mass, osmolarity, and volume in living cells |
title_full_unstemmed |
Label-free spatio-temporal monitoring of cytosolic mass, osmolarity, and volume in living cells |
title_sort |
label-free spatio-temporal monitoring of cytosolic mass, osmolarity, and volume in living cells |
publisher |
Nature Portfolio |
publishDate |
2019 |
url |
https://doaj.org/article/7beea7bfef8044bd83a66a1ec7e086bb |
work_keys_str_mv |
AT danielmidtvedt labelfreespatiotemporalmonitoringofcytosolicmassosmolarityandvolumeinlivingcells AT erikolsen labelfreespatiotemporalmonitoringofcytosolicmassosmolarityandvolumeinlivingcells AT fredrikhook labelfreespatiotemporalmonitoringofcytosolicmassosmolarityandvolumeinlivingcells AT gavindmjeffries labelfreespatiotemporalmonitoringofcytosolicmassosmolarityandvolumeinlivingcells |
_version_ |
1718382154163945472 |