Human intestinal dendritic cells decrease cytokine release against Salmonella infection in the presence of Lactobacillus paracasei upon TLR activation.

Probiotic bacteria have been shown to modulate immune responses and could have therapeutic effects in allergic and inflammatory disorders. However, little is known about the signalling pathways that are engaged by probiotics. Dendritic cells (DCs) are antigen-presenting cells that are involved in im...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Miriam Bermudez-Brito, Sergio Muñoz-Quezada, Carolina Gomez-Llorente, Esther Matencio, María J Bernal, Fernando Romero, Angel Gil
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7bf0a0b96fb54be2b0daef8494880791
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:7bf0a0b96fb54be2b0daef8494880791
record_format	dspace
spelling	oai:doaj.org-article:7bf0a0b96fb54be2b0daef84948807912021-11-18T07:08:48ZHuman intestinal dendritic cells decrease cytokine release against Salmonella infection in the presence of Lactobacillus paracasei upon TLR activation.1932-620310.1371/journal.pone.0043197https://doaj.org/article/7bf0a0b96fb54be2b0daef84948807912012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22905233/?tool=EBIhttps://doaj.org/toc/1932-6203Probiotic bacteria have been shown to modulate immune responses and could have therapeutic effects in allergic and inflammatory disorders. However, little is known about the signalling pathways that are engaged by probiotics. Dendritic cells (DCs) are antigen-presenting cells that are involved in immunity and tolerance. Monocyte-derived dendritic cells (MoDCs) and murine DCs are different from human gut DCs; therefore, in this study, we used human DCs generated from CD34+ progenitor cells (hematopoietic stem cells) harvested from umbilical cord blood; those DCs exhibited surface antigens of dendritic Langerhans cells, similar to the lamina propria DCs in the gut. We report that both a novel probiotic strain isolated from faeces of exclusively breast-fed newborn infants, Lactobacillus paracasei CNCM I-4034, and its cell-free culture supernatant (CFS) decreased pro-inflammatory cytokines and chemokines in human intestinal DCs challenged with Salmonella. Interestingly, the supernatant was as effective as the bacteria in reducing pro-inflammatory cytokine expression. In contrast, the bacterium was a potent inducer of TGF-β2 secretion, whereas the supernatant increased the secretion of TGF-β1 in response to Salmonella. We also showed that both the bacteria and its supernatant enhanced innate immunity through the activation of Toll-like receptor (TLR) signalling. These treatments strongly induced the transcription of the TLR9 gene. In addition, upregulation of the CASP8 and TOLLIP genes was observed. This work demonstrates that L. paracasei CNCM I-4034 enhanced innate immune responses, as evidenced by the activation of TLR signalling and the downregulation of a broad array of pro-inflammatory cytokines. The use of supernatants like the one described in this paper could be an effective and safe alternative to using live bacteria in functional foods.Miriam Bermudez-BritoSergio Muñoz-QuezadaCarolina Gomez-LlorenteEsther MatencioMaría J BernalFernando RomeroAngel GilPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 8, p e43197 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Miriam Bermudez-Brito Sergio Muñoz-Quezada Carolina Gomez-Llorente Esther Matencio María J Bernal Fernando Romero Angel Gil Human intestinal dendritic cells decrease cytokine release against Salmonella infection in the presence of Lactobacillus paracasei upon TLR activation.
description	Probiotic bacteria have been shown to modulate immune responses and could have therapeutic effects in allergic and inflammatory disorders. However, little is known about the signalling pathways that are engaged by probiotics. Dendritic cells (DCs) are antigen-presenting cells that are involved in immunity and tolerance. Monocyte-derived dendritic cells (MoDCs) and murine DCs are different from human gut DCs; therefore, in this study, we used human DCs generated from CD34+ progenitor cells (hematopoietic stem cells) harvested from umbilical cord blood; those DCs exhibited surface antigens of dendritic Langerhans cells, similar to the lamina propria DCs in the gut. We report that both a novel probiotic strain isolated from faeces of exclusively breast-fed newborn infants, Lactobacillus paracasei CNCM I-4034, and its cell-free culture supernatant (CFS) decreased pro-inflammatory cytokines and chemokines in human intestinal DCs challenged with Salmonella. Interestingly, the supernatant was as effective as the bacteria in reducing pro-inflammatory cytokine expression. In contrast, the bacterium was a potent inducer of TGF-β2 secretion, whereas the supernatant increased the secretion of TGF-β1 in response to Salmonella. We also showed that both the bacteria and its supernatant enhanced innate immunity through the activation of Toll-like receptor (TLR) signalling. These treatments strongly induced the transcription of the TLR9 gene. In addition, upregulation of the CASP8 and TOLLIP genes was observed. This work demonstrates that L. paracasei CNCM I-4034 enhanced innate immune responses, as evidenced by the activation of TLR signalling and the downregulation of a broad array of pro-inflammatory cytokines. The use of supernatants like the one described in this paper could be an effective and safe alternative to using live bacteria in functional foods.
format	article
author	Miriam Bermudez-Brito Sergio Muñoz-Quezada Carolina Gomez-Llorente Esther Matencio María J Bernal Fernando Romero Angel Gil
author_facet	Miriam Bermudez-Brito Sergio Muñoz-Quezada Carolina Gomez-Llorente Esther Matencio María J Bernal Fernando Romero Angel Gil
author_sort	Miriam Bermudez-Brito
title	Human intestinal dendritic cells decrease cytokine release against Salmonella infection in the presence of Lactobacillus paracasei upon TLR activation.
title_short	Human intestinal dendritic cells decrease cytokine release against Salmonella infection in the presence of Lactobacillus paracasei upon TLR activation.
title_full	Human intestinal dendritic cells decrease cytokine release against Salmonella infection in the presence of Lactobacillus paracasei upon TLR activation.
title_fullStr	Human intestinal dendritic cells decrease cytokine release against Salmonella infection in the presence of Lactobacillus paracasei upon TLR activation.
title_full_unstemmed	Human intestinal dendritic cells decrease cytokine release against Salmonella infection in the presence of Lactobacillus paracasei upon TLR activation.
title_sort	human intestinal dendritic cells decrease cytokine release against salmonella infection in the presence of lactobacillus paracasei upon tlr activation.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/7bf0a0b96fb54be2b0daef8494880791
work_keys_str_mv	AT miriambermudezbrito humanintestinaldendriticcellsdecreasecytokinereleaseagainstsalmonellainfectioninthepresenceoflactobacillusparacaseiupontlractivation AT sergiomunozquezada humanintestinaldendriticcellsdecreasecytokinereleaseagainstsalmonellainfectioninthepresenceoflactobacillusparacaseiupontlractivation AT carolinagomezllorente humanintestinaldendriticcellsdecreasecytokinereleaseagainstsalmonellainfectioninthepresenceoflactobacillusparacaseiupontlractivation AT esthermatencio humanintestinaldendriticcellsdecreasecytokinereleaseagainstsalmonellainfectioninthepresenceoflactobacillusparacaseiupontlractivation AT mariajbernal humanintestinaldendriticcellsdecreasecytokinereleaseagainstsalmonellainfectioninthepresenceoflactobacillusparacaseiupontlractivation AT fernandoromero humanintestinaldendriticcellsdecreasecytokinereleaseagainstsalmonellainfectioninthepresenceoflactobacillusparacaseiupontlractivation AT angelgil humanintestinaldendriticcellsdecreasecytokinereleaseagainstsalmonellainfectioninthepresenceoflactobacillusparacaseiupontlractivation
_version_	1718423859382714368

Human intestinal dendritic cells decrease cytokine release against Salmonella infection in the presence of Lactobacillus paracasei upon TLR activation.

Ejemplares similares