Population genetics and forensic utility of 23 autosomal PowerPlex Fusion 6C STR loci in the Kuwaiti population

Abstract This study evaluates the forensic utility of 23 autosomal short tandem repeat markers in 400 samples from the Kuwaiti population, of which four markers (D10S1248, D22S1045, D2S441 and SE33) are reported for the first time for Kuwait. All the markers were shown to exhibit no deviation from H...

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Autores principales:	Mahdi Haidar, Fatimah A. Abbas, Hussain Alsaleh, Penelope R. Haddrill
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7bf66bb6e86e484d980136dedc171bde
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spelling	oai:doaj.org-article:7bf66bb6e86e484d980136dedc171bde2021-12-02T13:51:06ZPopulation genetics and forensic utility of 23 autosomal PowerPlex Fusion 6C STR loci in the Kuwaiti population10.1038/s41598-021-81425-y2045-2322https://doaj.org/article/7bf66bb6e86e484d980136dedc171bde2021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-81425-yhttps://doaj.org/toc/2045-2322Abstract This study evaluates the forensic utility of 23 autosomal short tandem repeat markers in 400 samples from the Kuwaiti population, of which four markers (D10S1248, D22S1045, D2S441 and SE33) are reported for the first time for Kuwait. All the markers were shown to exhibit no deviation from Hardy–Weinberg equilibrium, nor any linkage disequilibrium between and within loci, indicating that these loci are inherited independently, and their allele frequencies can be used to estimate match probabilities in the Kuwaiti population. The low combined match probability of 7.37 × 10–30 and the high paternity indices generated by these loci demonstrate the usefulness of the PowerPlex Fusion 6C kit for human identification in this population, as well as to strengthen the power of paternity testing. Off-ladder alleles were seen at several loci, and these were identified by examining their underlying nucleotide sequences. Principal component analysis (PCA) and STRUCTURE showed no genetic structure within the Kuwaiti population. However, PCA revealed a correlation between geographic and genetic distance. Finally, phylogenetic trees demonstrated a close relationship between Kuwaitis and Middle Easterners at a global level, and a recent common ancestry for Kuwait with its northern neighbours of Iraq and Iran, at a regional level.Mahdi HaidarFatimah A. AbbasHussain AlsalehPenelope R. HaddrillNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mahdi Haidar Fatimah A. Abbas Hussain Alsaleh Penelope R. Haddrill Population genetics and forensic utility of 23 autosomal PowerPlex Fusion 6C STR loci in the Kuwaiti population
description	Abstract This study evaluates the forensic utility of 23 autosomal short tandem repeat markers in 400 samples from the Kuwaiti population, of which four markers (D10S1248, D22S1045, D2S441 and SE33) are reported for the first time for Kuwait. All the markers were shown to exhibit no deviation from Hardy–Weinberg equilibrium, nor any linkage disequilibrium between and within loci, indicating that these loci are inherited independently, and their allele frequencies can be used to estimate match probabilities in the Kuwaiti population. The low combined match probability of 7.37 × 10–30 and the high paternity indices generated by these loci demonstrate the usefulness of the PowerPlex Fusion 6C kit for human identification in this population, as well as to strengthen the power of paternity testing. Off-ladder alleles were seen at several loci, and these were identified by examining their underlying nucleotide sequences. Principal component analysis (PCA) and STRUCTURE showed no genetic structure within the Kuwaiti population. However, PCA revealed a correlation between geographic and genetic distance. Finally, phylogenetic trees demonstrated a close relationship between Kuwaitis and Middle Easterners at a global level, and a recent common ancestry for Kuwait with its northern neighbours of Iraq and Iran, at a regional level.
format	article
author	Mahdi Haidar Fatimah A. Abbas Hussain Alsaleh Penelope R. Haddrill
author_facet	Mahdi Haidar Fatimah A. Abbas Hussain Alsaleh Penelope R. Haddrill
author_sort	Mahdi Haidar
title	Population genetics and forensic utility of 23 autosomal PowerPlex Fusion 6C STR loci in the Kuwaiti population
title_short	Population genetics and forensic utility of 23 autosomal PowerPlex Fusion 6C STR loci in the Kuwaiti population
title_full	Population genetics and forensic utility of 23 autosomal PowerPlex Fusion 6C STR loci in the Kuwaiti population
title_fullStr	Population genetics and forensic utility of 23 autosomal PowerPlex Fusion 6C STR loci in the Kuwaiti population
title_full_unstemmed	Population genetics and forensic utility of 23 autosomal PowerPlex Fusion 6C STR loci in the Kuwaiti population
title_sort	population genetics and forensic utility of 23 autosomal powerplex fusion 6c str loci in the kuwaiti population
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/7bf66bb6e86e484d980136dedc171bde
work_keys_str_mv	AT mahdihaidar populationgeneticsandforensicutilityof23autosomalpowerplexfusion6cstrlociinthekuwaitipopulation AT fatimahaabbas populationgeneticsandforensicutilityof23autosomalpowerplexfusion6cstrlociinthekuwaitipopulation AT hussainalsaleh populationgeneticsandforensicutilityof23autosomalpowerplexfusion6cstrlociinthekuwaitipopulation AT peneloperhaddrill populationgeneticsandforensicutilityof23autosomalpowerplexfusion6cstrlociinthekuwaitipopulation
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Population genetics and forensic utility of 23 autosomal PowerPlex Fusion 6C STR loci in the Kuwaiti population

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