A single base-pair change in 2009 H1N1 hemagglutinin increases human receptor affinity and leads to efficient airborne viral transmission in ferrets.
The 2009 H1N1 influenza A virus continues to circulate among the human population as the predominant H1N1 subtype. Epidemiological studies and airborne transmission studies using the ferret model have shown that the transmission efficiency of 2009 H1N1 viruses is lower than that of previous seasonal...
Guardado en:
| Autores principales: | , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Public Library of Science (PLoS)
2011
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/7bfd197d7fb44d9fbb58304ffe14c61c |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:7bfd197d7fb44d9fbb58304ffe14c61c |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:7bfd197d7fb44d9fbb58304ffe14c61c2021-11-18T06:57:52ZA single base-pair change in 2009 H1N1 hemagglutinin increases human receptor affinity and leads to efficient airborne viral transmission in ferrets.1932-620310.1371/journal.pone.0017616https://doaj.org/article/7bfd197d7fb44d9fbb58304ffe14c61c2011-03-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21407805/?tool=EBIhttps://doaj.org/toc/1932-6203The 2009 H1N1 influenza A virus continues to circulate among the human population as the predominant H1N1 subtype. Epidemiological studies and airborne transmission studies using the ferret model have shown that the transmission efficiency of 2009 H1N1 viruses is lower than that of previous seasonal strains and the 1918 pandemic H1N1 strain. We recently correlated this reduced transmission efficiency to the lower binding affinity of the 2009 H1N1 hemagglutinin (HA) to α2→6 sialylated glycan receptors (human receptors). Here we report that a single point mutation (Ile219→Lys; a base pair change) in the glycan receptor-binding site (RBS) of a representative 2009 H1N1 influenza A virus, A/California/04/09 or CA04/09, quantitatively increases its human receptor-binding affinity. The increased human receptor-affinity is in the same range as that of the HA from highly transmissible seasonal and 1918 pandemic H1N1 viruses. Moreover, a 2009 H1N1 virus carrying this mutation in the RBS (generated using reverse genetics) transmits efficiently in ferrets by respiratory droplets thereby reestablishing our previously observed correlation between human receptor-binding affinity and transmission efficiency. These findings are significant in the context of monitoring the evolution of the currently circulating 2009 H1N1 viruses.Akila JayaramanClaudia PappasRahul RamanJessica A BelserKarthik ViswanathanZachary ShriverTerrence M TumpeyRam SasisekharanPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 3, p e17616 (2011) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Medicine R Science Q |
| spellingShingle |
Medicine R Science Q Akila Jayaraman Claudia Pappas Rahul Raman Jessica A Belser Karthik Viswanathan Zachary Shriver Terrence M Tumpey Ram Sasisekharan A single base-pair change in 2009 H1N1 hemagglutinin increases human receptor affinity and leads to efficient airborne viral transmission in ferrets. |
| description |
The 2009 H1N1 influenza A virus continues to circulate among the human population as the predominant H1N1 subtype. Epidemiological studies and airborne transmission studies using the ferret model have shown that the transmission efficiency of 2009 H1N1 viruses is lower than that of previous seasonal strains and the 1918 pandemic H1N1 strain. We recently correlated this reduced transmission efficiency to the lower binding affinity of the 2009 H1N1 hemagglutinin (HA) to α2→6 sialylated glycan receptors (human receptors). Here we report that a single point mutation (Ile219→Lys; a base pair change) in the glycan receptor-binding site (RBS) of a representative 2009 H1N1 influenza A virus, A/California/04/09 or CA04/09, quantitatively increases its human receptor-binding affinity. The increased human receptor-affinity is in the same range as that of the HA from highly transmissible seasonal and 1918 pandemic H1N1 viruses. Moreover, a 2009 H1N1 virus carrying this mutation in the RBS (generated using reverse genetics) transmits efficiently in ferrets by respiratory droplets thereby reestablishing our previously observed correlation between human receptor-binding affinity and transmission efficiency. These findings are significant in the context of monitoring the evolution of the currently circulating 2009 H1N1 viruses. |
| format |
article |
| author |
Akila Jayaraman Claudia Pappas Rahul Raman Jessica A Belser Karthik Viswanathan Zachary Shriver Terrence M Tumpey Ram Sasisekharan |
| author_facet |
Akila Jayaraman Claudia Pappas Rahul Raman Jessica A Belser Karthik Viswanathan Zachary Shriver Terrence M Tumpey Ram Sasisekharan |
| author_sort |
Akila Jayaraman |
| title |
A single base-pair change in 2009 H1N1 hemagglutinin increases human receptor affinity and leads to efficient airborne viral transmission in ferrets. |
| title_short |
A single base-pair change in 2009 H1N1 hemagglutinin increases human receptor affinity and leads to efficient airborne viral transmission in ferrets. |
| title_full |
A single base-pair change in 2009 H1N1 hemagglutinin increases human receptor affinity and leads to efficient airborne viral transmission in ferrets. |
| title_fullStr |
A single base-pair change in 2009 H1N1 hemagglutinin increases human receptor affinity and leads to efficient airborne viral transmission in ferrets. |
| title_full_unstemmed |
A single base-pair change in 2009 H1N1 hemagglutinin increases human receptor affinity and leads to efficient airborne viral transmission in ferrets. |
| title_sort |
single base-pair change in 2009 h1n1 hemagglutinin increases human receptor affinity and leads to efficient airborne viral transmission in ferrets. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2011 |
| url |
https://doaj.org/article/7bfd197d7fb44d9fbb58304ffe14c61c |
| work_keys_str_mv |
AT akilajayaraman asinglebasepairchangein2009h1n1hemagglutininincreaseshumanreceptoraffinityandleadstoefficientairborneviraltransmissioninferrets AT claudiapappas asinglebasepairchangein2009h1n1hemagglutininincreaseshumanreceptoraffinityandleadstoefficientairborneviraltransmissioninferrets AT rahulraman asinglebasepairchangein2009h1n1hemagglutininincreaseshumanreceptoraffinityandleadstoefficientairborneviraltransmissioninferrets AT jessicaabelser asinglebasepairchangein2009h1n1hemagglutininincreaseshumanreceptoraffinityandleadstoefficientairborneviraltransmissioninferrets AT karthikviswanathan asinglebasepairchangein2009h1n1hemagglutininincreaseshumanreceptoraffinityandleadstoefficientairborneviraltransmissioninferrets AT zacharyshriver asinglebasepairchangein2009h1n1hemagglutininincreaseshumanreceptoraffinityandleadstoefficientairborneviraltransmissioninferrets AT terrencemtumpey asinglebasepairchangein2009h1n1hemagglutininincreaseshumanreceptoraffinityandleadstoefficientairborneviraltransmissioninferrets AT ramsasisekharan asinglebasepairchangein2009h1n1hemagglutininincreaseshumanreceptoraffinityandleadstoefficientairborneviraltransmissioninferrets AT akilajayaraman singlebasepairchangein2009h1n1hemagglutininincreaseshumanreceptoraffinityandleadstoefficientairborneviraltransmissioninferrets AT claudiapappas singlebasepairchangein2009h1n1hemagglutininincreaseshumanreceptoraffinityandleadstoefficientairborneviraltransmissioninferrets AT rahulraman singlebasepairchangein2009h1n1hemagglutininincreaseshumanreceptoraffinityandleadstoefficientairborneviraltransmissioninferrets AT jessicaabelser singlebasepairchangein2009h1n1hemagglutininincreaseshumanreceptoraffinityandleadstoefficientairborneviraltransmissioninferrets AT karthikviswanathan singlebasepairchangein2009h1n1hemagglutininincreaseshumanreceptoraffinityandleadstoefficientairborneviraltransmissioninferrets AT zacharyshriver singlebasepairchangein2009h1n1hemagglutininincreaseshumanreceptoraffinityandleadstoefficientairborneviraltransmissioninferrets AT terrencemtumpey singlebasepairchangein2009h1n1hemagglutininincreaseshumanreceptoraffinityandleadstoefficientairborneviraltransmissioninferrets AT ramsasisekharan singlebasepairchangein2009h1n1hemagglutininincreaseshumanreceptoraffinityandleadstoefficientairborneviraltransmissioninferrets |
| _version_ |
1718424113583751168 |