Water-soluble fullerene (c60) inhibits the development of arthritis in the rat model of arthritis

Kazuo Yudoh1, Rie Karasawa1, Kayo Masuko2, Tomohiro Kato2 1Institute of Medical Science, 2Department of Biochemistry, St. Marianna University School of Medicine, Kawasaki, JapanAbstract: Recently, it has been demonstrated that oxygen free radicals have an important role as a signaling messenger in t...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Kazuo Yudoh, Rie Karasawa, Kayo Masuko, et al.&nbsp;
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2009
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/7c008bb2352b42b4b1cae9de965a4bbb
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:7c008bb2352b42b4b1cae9de965a4bbb
record_format	dspace
spelling	oai:doaj.org-article:7c008bb2352b42b4b1cae9de965a4bbb2021-12-02T02:51:10ZWater-soluble fullerene (c60) inhibits the development of arthritis in the rat model of arthritis1176-91141178-2013https://doaj.org/article/7c008bb2352b42b4b1cae9de965a4bbb2009-10-01T00:00:00Zhttp://www.dovepress.com/water-soluble-fullerene-c60-inhibits-the-development-of-arthritis-in-t-a3642https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Kazuo Yudoh1, Rie Karasawa1, Kayo Masuko2, Tomohiro Kato2 1Institute of Medical Science, 2Department of Biochemistry, St. Marianna University School of Medicine, Kawasaki, JapanAbstract: Recently, it has been demonstrated that oxygen free radicals have an important role as a signaling messenger in the development of inflammation and osteoclastogenesis, suggesting the implication of oxygen free radicals in the pathogenesis of arthritis. The aim of this study was to examine the potential of a strong free-radical scavenger, water-soluble fullerene (C60), as a protective agent against synovitis in arthritis, both in vitro and in vivo. In the presence or absence of C60 (0.1, 1.0, 10.0 &micro;M), human synovial fibroblasts, synovial infiltrating lymphocytes or macrophages were incubated with tumor necrosis factor-&alpha; (TNF-&alpha;) (10.0 ng/mL), and the production of proinflammatory cytokines by the individual cells were analyzed. C60 significantly suppressed the TNF-&alpha;-induced production of proinflammatory cytokines in synovial fibroblasts, synovial infiltrating lymphocytes and macrophages in vitro. Adjuvant induced arthritic rats were used as an animal model of arthritis. Rats were divided into two subgroups: control and treatment with C60 at 10.0 &micro;M. The left ankle joint was injected intraarticularly with water-soluble C60 (20 &micro;l) in the C60-treated group, while, as a control, the left ankle joint in the control rats received phosphate-buffered saline (20 &micro;l), once weekly for eight weeks. Ankle joint tissues were prepared for histological analysis. In adjuvant-induced arthritic rats, intra-articular treatment with C60 in vivo reduced synovitis and alleviated bone resorption and destruction in the joints, while control ankle joints showed progression of synovitis and joint destruction with time. These findings indicate that C60 is a potential therapeutic agent for inhibition of arthritis.Keywords: fullerene, inflammation, arthritis, synovitis, bone resorption Kazuo YudohRie KarasawaKayo Masukoet al.&nbsp;Dove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2009, Iss default, Pp 217-225 (2009)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Kazuo Yudoh Rie Karasawa Kayo Masuko et al.&nbsp; Water-soluble fullerene (c60) inhibits the development of arthritis in the rat model of arthritis
description	Kazuo Yudoh1, Rie Karasawa1, Kayo Masuko2, Tomohiro Kato2 1Institute of Medical Science, 2Department of Biochemistry, St. Marianna University School of Medicine, Kawasaki, JapanAbstract: Recently, it has been demonstrated that oxygen free radicals have an important role as a signaling messenger in the development of inflammation and osteoclastogenesis, suggesting the implication of oxygen free radicals in the pathogenesis of arthritis. The aim of this study was to examine the potential of a strong free-radical scavenger, water-soluble fullerene (C60), as a protective agent against synovitis in arthritis, both in vitro and in vivo. In the presence or absence of C60 (0.1, 1.0, 10.0 &micro;M), human synovial fibroblasts, synovial infiltrating lymphocytes or macrophages were incubated with tumor necrosis factor-&alpha; (TNF-&alpha;) (10.0 ng/mL), and the production of proinflammatory cytokines by the individual cells were analyzed. C60 significantly suppressed the TNF-&alpha;-induced production of proinflammatory cytokines in synovial fibroblasts, synovial infiltrating lymphocytes and macrophages in vitro. Adjuvant induced arthritic rats were used as an animal model of arthritis. Rats were divided into two subgroups: control and treatment with C60 at 10.0 &micro;M. The left ankle joint was injected intraarticularly with water-soluble C60 (20 &micro;l) in the C60-treated group, while, as a control, the left ankle joint in the control rats received phosphate-buffered saline (20 &micro;l), once weekly for eight weeks. Ankle joint tissues were prepared for histological analysis. In adjuvant-induced arthritic rats, intra-articular treatment with C60 in vivo reduced synovitis and alleviated bone resorption and destruction in the joints, while control ankle joints showed progression of synovitis and joint destruction with time. These findings indicate that C60 is a potential therapeutic agent for inhibition of arthritis.Keywords: fullerene, inflammation, arthritis, synovitis, bone resorption
format	article
author	Kazuo Yudoh Rie Karasawa Kayo Masuko et al.&nbsp;
author_facet	Kazuo Yudoh Rie Karasawa Kayo Masuko et al.&nbsp;
author_sort	Kazuo Yudoh
title	Water-soluble fullerene (c60) inhibits the development of arthritis in the rat model of arthritis
title_short	Water-soluble fullerene (c60) inhibits the development of arthritis in the rat model of arthritis
title_full	Water-soluble fullerene (c60) inhibits the development of arthritis in the rat model of arthritis
title_fullStr	Water-soluble fullerene (c60) inhibits the development of arthritis in the rat model of arthritis
title_full_unstemmed	Water-soluble fullerene (c60) inhibits the development of arthritis in the rat model of arthritis
title_sort	water-soluble fullerene (c60) inhibits the development of arthritis in the rat model of arthritis
publisher	Dove Medical Press
publishDate	2009
url	https://doaj.org/article/7c008bb2352b42b4b1cae9de965a4bbb
work_keys_str_mv	AT kazuoyudoh watersolublefullerenec60inhibitsthedevelopmentofarthritisintheratmodelofarthritis AT riekarasawa watersolublefullerenec60inhibitsthedevelopmentofarthritisintheratmodelofarthritis AT kayomasuko watersolublefullerenec60inhibitsthedevelopmentofarthritisintheratmodelofarthritis AT etalampnbsp watersolublefullerenec60inhibitsthedevelopmentofarthritisintheratmodelofarthritis
_version_	1718402108880846848

Water-soluble fullerene (c60) inhibits the development of arthritis in the rat model of arthritis

Ejemplares similares