Effects of co-exposure to copper and humic acids on microalga Chlorella vulgaris: growth inhibition, oxidative stress, and extracellular secretion

Heavy metals were typical contaminants in aquatic ecosystems, while their effects on the biotoxicity and metabolism of algal cells in the presence of organic ligands were still unclear. In this study, the contrasting effects of Cu and humic acids (HA) on the growth inhibition, oxidative stress and e...

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Autores principales:	Zhiqiang Shi, Huacheng Xu, Zhiyuan Wang, Haiyan Du, Xiaowei Fu
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	cu toxicity humic acids toxicity alleviation extracellular polymeric substance oxidative stress Environmental pollution TD172-193.5 Environmental sciences GE1-350
Acceso en línea:	https://doaj.org/article/7c016dcfcedd497582ab644a51f30d0e
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spelling	oai:doaj.org-article:7c016dcfcedd497582ab644a51f30d0e2021-11-11T14:23:43ZEffects of co-exposure to copper and humic acids on microalga Chlorella vulgaris: growth inhibition, oxidative stress, and extracellular secretion2639-594010.1080/26395940.2021.1995504https://doaj.org/article/7c016dcfcedd497582ab644a51f30d0e2021-01-01T00:00:00Zhttp://dx.doi.org/10.1080/26395940.2021.1995504https://doaj.org/toc/2639-5940Heavy metals were typical contaminants in aquatic ecosystems, while their effects on the biotoxicity and metabolism of algal cells in the presence of organic ligands were still unclear. In this study, the contrasting effects of Cu and humic acids (HA) on the growth inhibition, oxidative stress and extracellular secretion of microalga Chlorella vulgaris were studied at environmentally relevant concentrations of Cu (0~2 mg/L) and HA (0~20 mg/L). Results showed that, with increased Cu addition, the algal growth rate decreased while concentrations of superoxide dismutase and malondialdehyde increased showing evident growth inhibition and oxidative damage in the sole presence of Cu. However, the presence of HA could significantly alleviate the Cu-induced toxicity damage, and higher concentrations of HA exhibited greater alleviation efficiencies. Fluorescence spectroscopy combined with flow field flow fractionation revealed that secretion of 50 kDa~0.45 μm of extracellular protein-like substances was the critical fractions responsible for the biotoxicity alleviation. Analysis of Cu contents within extracellular polymeric substance (EPS) matrix showed that not only the secretion amounts but also the acquisition capacities of EPS matrix decreased due to the addition of HA. This study benefited an in-depth understanding toward the toxicity of heavy metals to aquatic microorganisms in natural waters that contained abundant organic ligands.Zhiqiang ShiHuacheng XuZhiyuan WangHaiyan DuXiaowei FuTaylor & Francis Grouparticlecu toxicityhumic acidstoxicity alleviationextracellular polymeric substanceoxidative stressEnvironmental pollutionTD172-193.5Environmental sciencesGE1-350ENEnvironmental Pollutants & Bioavailability, Vol 33, Iss 1, Pp 415-424 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	cu toxicity humic acids toxicity alleviation extracellular polymeric substance oxidative stress Environmental pollution TD172-193.5 Environmental sciences GE1-350
spellingShingle	cu toxicity humic acids toxicity alleviation extracellular polymeric substance oxidative stress Environmental pollution TD172-193.5 Environmental sciences GE1-350 Zhiqiang Shi Huacheng Xu Zhiyuan Wang Haiyan Du Xiaowei Fu Effects of co-exposure to copper and humic acids on microalga Chlorella vulgaris: growth inhibition, oxidative stress, and extracellular secretion
description	Heavy metals were typical contaminants in aquatic ecosystems, while their effects on the biotoxicity and metabolism of algal cells in the presence of organic ligands were still unclear. In this study, the contrasting effects of Cu and humic acids (HA) on the growth inhibition, oxidative stress and extracellular secretion of microalga Chlorella vulgaris were studied at environmentally relevant concentrations of Cu (0~2 mg/L) and HA (0~20 mg/L). Results showed that, with increased Cu addition, the algal growth rate decreased while concentrations of superoxide dismutase and malondialdehyde increased showing evident growth inhibition and oxidative damage in the sole presence of Cu. However, the presence of HA could significantly alleviate the Cu-induced toxicity damage, and higher concentrations of HA exhibited greater alleviation efficiencies. Fluorescence spectroscopy combined with flow field flow fractionation revealed that secretion of 50 kDa~0.45 μm of extracellular protein-like substances was the critical fractions responsible for the biotoxicity alleviation. Analysis of Cu contents within extracellular polymeric substance (EPS) matrix showed that not only the secretion amounts but also the acquisition capacities of EPS matrix decreased due to the addition of HA. This study benefited an in-depth understanding toward the toxicity of heavy metals to aquatic microorganisms in natural waters that contained abundant organic ligands.
format	article
author	Zhiqiang Shi Huacheng Xu Zhiyuan Wang Haiyan Du Xiaowei Fu
author_facet	Zhiqiang Shi Huacheng Xu Zhiyuan Wang Haiyan Du Xiaowei Fu
author_sort	Zhiqiang Shi
title	Effects of co-exposure to copper and humic acids on microalga Chlorella vulgaris: growth inhibition, oxidative stress, and extracellular secretion
title_short	Effects of co-exposure to copper and humic acids on microalga Chlorella vulgaris: growth inhibition, oxidative stress, and extracellular secretion
title_full	Effects of co-exposure to copper and humic acids on microalga Chlorella vulgaris: growth inhibition, oxidative stress, and extracellular secretion
title_fullStr	Effects of co-exposure to copper and humic acids on microalga Chlorella vulgaris: growth inhibition, oxidative stress, and extracellular secretion
title_full_unstemmed	Effects of co-exposure to copper and humic acids on microalga Chlorella vulgaris: growth inhibition, oxidative stress, and extracellular secretion
title_sort	effects of co-exposure to copper and humic acids on microalga chlorella vulgaris: growth inhibition, oxidative stress, and extracellular secretion
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/7c016dcfcedd497582ab644a51f30d0e
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Effects of co-exposure to copper and humic acids on microalga Chlorella vulgaris: growth inhibition, oxidative stress, and extracellular secretion

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