The dynamic interacting landscape of MAPL reveals essential functions for SUMOylation in innate immunity

Abstract Activation of the innate immune response triggered by dsRNA viruses occurs through the assembly of the Mitochondrial Anti-Viral Signaling (MAVS) complex. Upon recognition of viral dsRNA, the cytosolic receptor RIG-I is activated and recruited to MAVS to activate the immune signaling respons...

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Autores principales:	Karine Doiron, Vanessa Goyon, Etienne Coyaud, Sanjeeva Rajapakse, Brian Raught, Heidi M. McBride
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7c05e8c330a54cc0a18d9dbc8e53f90c
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spelling	oai:doaj.org-article:7c05e8c330a54cc0a18d9dbc8e53f90c2021-12-02T16:06:29ZThe dynamic interacting landscape of MAPL reveals essential functions for SUMOylation in innate immunity10.1038/s41598-017-00151-62045-2322https://doaj.org/article/7c05e8c330a54cc0a18d9dbc8e53f90c2017-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00151-6https://doaj.org/toc/2045-2322Abstract Activation of the innate immune response triggered by dsRNA viruses occurs through the assembly of the Mitochondrial Anti-Viral Signaling (MAVS) complex. Upon recognition of viral dsRNA, the cytosolic receptor RIG-I is activated and recruited to MAVS to activate the immune signaling response. We here demonstrate a strict requirement for a mitochondrial anchored protein ligase, MAPL (also called MUL1) in the signaling events that drive the transcriptional activation of antiviral genes downstream of Sendai virus infection, both in vivo and in vitro. A biotin environment scan of MAPL interacting polypeptides identified a series of proteins specific to Sendai virus infection; including RIG-I, IFIT1, IFIT2, HERC5 and others. Upon infection, RIG-I is SUMOylated in a MAPL-dependent manner, a conjugation step that is required for its activation. Consistent with this, MAPL was not required for signaling downstream of a constitutively activated form of RIG-I. These data highlight a critical role for MAPL and mitochondrial SUMOylation in the early steps of antiviral signaling.Karine DoironVanessa GoyonEtienne CoyaudSanjeeva RajapakseBrian RaughtHeidi M. McBrideNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Karine Doiron Vanessa Goyon Etienne Coyaud Sanjeeva Rajapakse Brian Raught Heidi M. McBride The dynamic interacting landscape of MAPL reveals essential functions for SUMOylation in innate immunity
description	Abstract Activation of the innate immune response triggered by dsRNA viruses occurs through the assembly of the Mitochondrial Anti-Viral Signaling (MAVS) complex. Upon recognition of viral dsRNA, the cytosolic receptor RIG-I is activated and recruited to MAVS to activate the immune signaling response. We here demonstrate a strict requirement for a mitochondrial anchored protein ligase, MAPL (also called MUL1) in the signaling events that drive the transcriptional activation of antiviral genes downstream of Sendai virus infection, both in vivo and in vitro. A biotin environment scan of MAPL interacting polypeptides identified a series of proteins specific to Sendai virus infection; including RIG-I, IFIT1, IFIT2, HERC5 and others. Upon infection, RIG-I is SUMOylated in a MAPL-dependent manner, a conjugation step that is required for its activation. Consistent with this, MAPL was not required for signaling downstream of a constitutively activated form of RIG-I. These data highlight a critical role for MAPL and mitochondrial SUMOylation in the early steps of antiviral signaling.
format	article
author	Karine Doiron Vanessa Goyon Etienne Coyaud Sanjeeva Rajapakse Brian Raught Heidi M. McBride
author_facet	Karine Doiron Vanessa Goyon Etienne Coyaud Sanjeeva Rajapakse Brian Raught Heidi M. McBride
author_sort	Karine Doiron
title	The dynamic interacting landscape of MAPL reveals essential functions for SUMOylation in innate immunity
title_short	The dynamic interacting landscape of MAPL reveals essential functions for SUMOylation in innate immunity
title_full	The dynamic interacting landscape of MAPL reveals essential functions for SUMOylation in innate immunity
title_fullStr	The dynamic interacting landscape of MAPL reveals essential functions for SUMOylation in innate immunity
title_full_unstemmed	The dynamic interacting landscape of MAPL reveals essential functions for SUMOylation in innate immunity
title_sort	dynamic interacting landscape of mapl reveals essential functions for sumoylation in innate immunity
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/7c05e8c330a54cc0a18d9dbc8e53f90c
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The dynamic interacting landscape of MAPL reveals essential functions for SUMOylation in innate immunity

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