Molecular cloning, characterization and expression profile of the sucrose synthase gene family in Litchi chinensis

Sucrose synthase (SUS, EC 2.4.1.13) is widely considered as a key enzyme involved in plant sucrose metabolism, and the gene family encoding different SUS isozymes has been identified and characterized in several plant species. However, to date scant information about the SUS genes is available in Li...

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Autores principales:	Dan Wang, Jietang Zhao, Yaqi Qin, Yonghua Qin, Guibing Hu
Formato:	article
Lenguaje:	EN
Publicado:	KeAi Communications Co., Ltd. 2021
Materias:	Litchi chinensis Sucrose synthase Gene expression Aril Sugar accumulation Plant culture SB1-1110
Acceso en línea:	https://doaj.org/article/7c092f1f674044dd9d837d48cff4578c
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spelling	oai:doaj.org-article:7c092f1f674044dd9d837d48cff4578c2021-11-12T04:43:22ZMolecular cloning, characterization and expression profile of the sucrose synthase gene family in Litchi chinensis2468-014110.1016/j.hpj.2021.04.004https://doaj.org/article/7c092f1f674044dd9d837d48cff4578c2021-11-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2468014121000522https://doaj.org/toc/2468-0141Sucrose synthase (SUS, EC 2.4.1.13) is widely considered as a key enzyme involved in plant sucrose metabolism, and the gene family encoding different SUS isozymes has been identified and characterized in several plant species. However, to date scant information about the SUS genes is available in Litchi chinensis Sonn. Here, we identified five SUS genes in litchi. These LcSUSs shared high levels of similarity in both nucleotide and amino acid sequences. Their gene structure, phylogenetic relationships, and expression profiles were characterized. Gene structure analysis indicated that the LcSUSs have similar exon-intron structures. Phylogenetic analysis revealed that the five members could be classified into three groups (LcSUS1 and LcSUS2 in SUS II, LcSUS4 and LcSUS5 in SUS III, and LcSUS3 in SUS I), demonstrating evolutionary conservation in the SUS family across litchi and other plant species. The expression levels of LcSUSs were investigated via real-time PCR in various tissues and different developmental stages of aril. For tissues and organs, LcSUSs exhibited distinct but partially redundant expression profiles in litchi, being predominantly expressed in young leaves (sink). During aril development, the expression pattern of LcSUS1 was consistent with the trend of sugar accumulation, indicating it may play important roles in determination of sink strength in aril. Moreover, transcript levels of LcSUS2, LcSUS4, and LcSUS5 varied between cultivars with different hexose/sucrose ratios, which may regulate the sugar composition in aril. Our results provide insights into physiological functions of SUS genes in litchi, especially roles in regulating sugar accumulation in aril.Dan WangJietang ZhaoYaqi QinYonghua QinGuibing HuKeAi Communications Co., Ltd.articleLitchi chinensisSucrose synthaseGene expressionArilSugar accumulationPlant cultureSB1-1110ENHorticultural Plant Journal, Vol 7, Iss 6, Pp 520-528 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Litchi chinensis Sucrose synthase Gene expression Aril Sugar accumulation Plant culture SB1-1110
spellingShingle	Litchi chinensis Sucrose synthase Gene expression Aril Sugar accumulation Plant culture SB1-1110 Dan Wang Jietang Zhao Yaqi Qin Yonghua Qin Guibing Hu Molecular cloning, characterization and expression profile of the sucrose synthase gene family in Litchi chinensis
description	Sucrose synthase (SUS, EC 2.4.1.13) is widely considered as a key enzyme involved in plant sucrose metabolism, and the gene family encoding different SUS isozymes has been identified and characterized in several plant species. However, to date scant information about the SUS genes is available in Litchi chinensis Sonn. Here, we identified five SUS genes in litchi. These LcSUSs shared high levels of similarity in both nucleotide and amino acid sequences. Their gene structure, phylogenetic relationships, and expression profiles were characterized. Gene structure analysis indicated that the LcSUSs have similar exon-intron structures. Phylogenetic analysis revealed that the five members could be classified into three groups (LcSUS1 and LcSUS2 in SUS II, LcSUS4 and LcSUS5 in SUS III, and LcSUS3 in SUS I), demonstrating evolutionary conservation in the SUS family across litchi and other plant species. The expression levels of LcSUSs were investigated via real-time PCR in various tissues and different developmental stages of aril. For tissues and organs, LcSUSs exhibited distinct but partially redundant expression profiles in litchi, being predominantly expressed in young leaves (sink). During aril development, the expression pattern of LcSUS1 was consistent with the trend of sugar accumulation, indicating it may play important roles in determination of sink strength in aril. Moreover, transcript levels of LcSUS2, LcSUS4, and LcSUS5 varied between cultivars with different hexose/sucrose ratios, which may regulate the sugar composition in aril. Our results provide insights into physiological functions of SUS genes in litchi, especially roles in regulating sugar accumulation in aril.
format	article
author	Dan Wang Jietang Zhao Yaqi Qin Yonghua Qin Guibing Hu
author_facet	Dan Wang Jietang Zhao Yaqi Qin Yonghua Qin Guibing Hu
author_sort	Dan Wang
title	Molecular cloning, characterization and expression profile of the sucrose synthase gene family in Litchi chinensis
title_short	Molecular cloning, characterization and expression profile of the sucrose synthase gene family in Litchi chinensis
title_full	Molecular cloning, characterization and expression profile of the sucrose synthase gene family in Litchi chinensis
title_fullStr	Molecular cloning, characterization and expression profile of the sucrose synthase gene family in Litchi chinensis
title_full_unstemmed	Molecular cloning, characterization and expression profile of the sucrose synthase gene family in Litchi chinensis
title_sort	molecular cloning, characterization and expression profile of the sucrose synthase gene family in litchi chinensis
publisher	KeAi Communications Co., Ltd.
publishDate	2021
url	https://doaj.org/article/7c092f1f674044dd9d837d48cff4578c
work_keys_str_mv	AT danwang molecularcloningcharacterizationandexpressionprofileofthesucrosesynthasegenefamilyinlitchichinensis AT jietangzhao molecularcloningcharacterizationandexpressionprofileofthesucrosesynthasegenefamilyinlitchichinensis AT yaqiqin molecularcloningcharacterizationandexpressionprofileofthesucrosesynthasegenefamilyinlitchichinensis AT yonghuaqin molecularcloningcharacterizationandexpressionprofileofthesucrosesynthasegenefamilyinlitchichinensis AT guibinghu molecularcloningcharacterizationandexpressionprofileofthesucrosesynthasegenefamilyinlitchichinensis
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Molecular cloning, characterization and expression profile of the sucrose synthase gene family in Litchi chinensis

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