Development of custom Surface Plasmon Resonance Au biosensor for liver cancer biomarker detection

Label-free plasmonic biosensors have demonstrated promising capabilities as diagnostic tools for the detection of different target analytes. The overall analytical sensitivity and specificity of these systems depend on the sensor surface biofunctionalization, since it influences their interactions w...

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Autores principales:	Sarassunta Ucci, Paola Cicatiello, Sara Spaziani, Andrea Cusano
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Biosensing Liquid biopsy Surface Plasmon Resonance Cancer biomarkers Optics. Light QC350-467
Acceso en línea:	https://doaj.org/article/7c13dacc24804d28a0872a481939f8b2
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spelling	oai:doaj.org-article:7c13dacc24804d28a0872a481939f8b22021-11-28T04:39:37ZDevelopment of custom Surface Plasmon Resonance Au biosensor for liver cancer biomarker detection2666-950110.1016/j.rio.2021.100193https://doaj.org/article/7c13dacc24804d28a0872a481939f8b22021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2666950121001383https://doaj.org/toc/2666-9501Label-free plasmonic biosensors have demonstrated promising capabilities as diagnostic tools for the detection of different target analytes. The overall analytical sensitivity and specificity of these systems depend on the sensor surface biofunctionalization, since it influences their interactions with the target molecules and determines their applicability to biomolecular detection in complex matrices. In this study, we report the development of Au biosensor by integrating the immunosandwich sensing approach into a Surface Plasmon Resonance (SPR) system for detecting cancer biomarkers at a concentration as low as the picomolar range. Our work has demonstrated that antibody-functionalized sensing film, together with signal amplification by detection antibody, was readily served as a plasmonic partner that can be used as a powerful ultrasensitive sandwich immunoassay for cancer-related disease diagnosis. The proposed Au biosensor is designed with high detection accuracy and sensitivity for alpha-fetoprotein (AFP), a well-known liver cancer biomarker. AFP detection performed by our Au biosensor provides an impressive limit of detection equal to 1.5 ng/ml in a saline buffer, lower than the relevant clinic concentration. The data reported here can contribute to the development of label-free device that can be freely functionalized to obtain an even better recognition of different biomarkers in real-time and with high reproducibility thanks to the possibility of being used after regeneration, offering an effective option for cancer screening suitable in point-of-care (POC) testing.Sarassunta UcciPaola CicatielloSara SpazianiAndrea CusanoElsevierarticleBiosensingLiquid biopsySurface Plasmon ResonanceCancer biomarkersOptics. LightQC350-467ENResults in Optics, Vol 5, Iss , Pp 100193- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biosensing Liquid biopsy Surface Plasmon Resonance Cancer biomarkers Optics. Light QC350-467
spellingShingle	Biosensing Liquid biopsy Surface Plasmon Resonance Cancer biomarkers Optics. Light QC350-467 Sarassunta Ucci Paola Cicatiello Sara Spaziani Andrea Cusano Development of custom Surface Plasmon Resonance Au biosensor for liver cancer biomarker detection
description	Label-free plasmonic biosensors have demonstrated promising capabilities as diagnostic tools for the detection of different target analytes. The overall analytical sensitivity and specificity of these systems depend on the sensor surface biofunctionalization, since it influences their interactions with the target molecules and determines their applicability to biomolecular detection in complex matrices. In this study, we report the development of Au biosensor by integrating the immunosandwich sensing approach into a Surface Plasmon Resonance (SPR) system for detecting cancer biomarkers at a concentration as low as the picomolar range. Our work has demonstrated that antibody-functionalized sensing film, together with signal amplification by detection antibody, was readily served as a plasmonic partner that can be used as a powerful ultrasensitive sandwich immunoassay for cancer-related disease diagnosis. The proposed Au biosensor is designed with high detection accuracy and sensitivity for alpha-fetoprotein (AFP), a well-known liver cancer biomarker. AFP detection performed by our Au biosensor provides an impressive limit of detection equal to 1.5 ng/ml in a saline buffer, lower than the relevant clinic concentration. The data reported here can contribute to the development of label-free device that can be freely functionalized to obtain an even better recognition of different biomarkers in real-time and with high reproducibility thanks to the possibility of being used after regeneration, offering an effective option for cancer screening suitable in point-of-care (POC) testing.
format	article
author	Sarassunta Ucci Paola Cicatiello Sara Spaziani Andrea Cusano
author_facet	Sarassunta Ucci Paola Cicatiello Sara Spaziani Andrea Cusano
author_sort	Sarassunta Ucci
title	Development of custom Surface Plasmon Resonance Au biosensor for liver cancer biomarker detection
title_short	Development of custom Surface Plasmon Resonance Au biosensor for liver cancer biomarker detection
title_full	Development of custom Surface Plasmon Resonance Au biosensor for liver cancer biomarker detection
title_fullStr	Development of custom Surface Plasmon Resonance Au biosensor for liver cancer biomarker detection
title_full_unstemmed	Development of custom Surface Plasmon Resonance Au biosensor for liver cancer biomarker detection
title_sort	development of custom surface plasmon resonance au biosensor for liver cancer biomarker detection
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/7c13dacc24804d28a0872a481939f8b2
work_keys_str_mv	AT sarassuntaucci developmentofcustomsurfaceplasmonresonanceaubiosensorforlivercancerbiomarkerdetection AT paolacicatiello developmentofcustomsurfaceplasmonresonanceaubiosensorforlivercancerbiomarkerdetection AT saraspaziani developmentofcustomsurfaceplasmonresonanceaubiosensorforlivercancerbiomarkerdetection AT andreacusano developmentofcustomsurfaceplasmonresonanceaubiosensorforlivercancerbiomarkerdetection
_version_	1718408268125044736

Development of custom Surface Plasmon Resonance Au biosensor for liver cancer biomarker detection

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