Pomegranate extract-loaded solid lipid nanoparticles: design, optimization, and in vitro cytotoxicity study

Noha M Badawi,1 Mahmoud H Teaima,2 Khalid M El-Say,3 Dalia A Attia,1 Mohamed A El-Nabarawi,2 Mohey M Elmazar4 1Department of Pharmaceutics, Faculty of Pharmacy, The British University in Egypt, Cairo, Egypt; 2Department of Pharmaceutics and Industrial Pharmacy, Cairo University, Cairo, Egy...

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Autores principales:	Badawi NM, Teaima MH, El-Say KM, Attia DA, El-Nabarawi MA, Elmazar MM
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2018
Materias:	Pomegranate extract Solid lipid nanoparticles Box–Behnken design optimization cancer cell lines Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/7c155d184abc457db8b1bf771731f732
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spelling	oai:doaj.org-article:7c155d184abc457db8b1bf771731f7322021-12-02T02:42:32ZPomegranate extract-loaded solid lipid nanoparticles: design, optimization, and in vitro cytotoxicity study1178-2013https://doaj.org/article/7c155d184abc457db8b1bf771731f7322018-03-01T00:00:00Zhttps://www.dovepress.com/pomegranate-extract-loaded-solid-lipid-nanoparticles-design-optimizati-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Noha M Badawi,1 Mahmoud H Teaima,2 Khalid M El-Say,3 Dalia A Attia,1 Mohamed A El-Nabarawi,2 Mohey M Elmazar4 1Department of Pharmaceutics, Faculty of Pharmacy, The British University in Egypt, Cairo, Egypt; 2Department of Pharmaceutics and Industrial Pharmacy, Cairo University, Cairo, Egypt; 3Department of Pharmaceutics, King Abdulaziz University, Jeddah, Saudi Arabia; 4Department of Pharmacology, Faculty of Pharmacy, The British University in Egypt, Cairo, Egypt Background: Pomegranate extract (PE) is a natural product with potent antioxidant and anticancer activity because of its polyphenols content. The main purpose of this study was to maximize the PE chemotherapeutic efficacy by loading it in an optimized solid lipid nanoparticles (SLNs) formula.Materials and methods: The influence of independent variables, which were lipid concentration (X1), surfactant concentration (X2) and cosurfactant concentration (X3), on dependent ones, which were particle size (Y1), polydispersity index (Y2), zeta potential (Y3), entrapment efficiency (Y4) and cumulative % drug release (Y5), were studied and optimized using the Box–Behnken design. Fifteen formulations of PE-SLNs were prepared using hot homogenization followed by ultra-sonication technique. Response surface plots, Pareto charts and mathematical equations were produced to study the impact of independent variables on the dependent quality parameters. The anti-proliferative activity of the optimized formula was then evaluated in three different cancer cell lines, namely, MCF-7, PC-3 and HepG-2, in addition to one normal cell line, HFB-4.Results: The results demonstrated that the particle sizes ranged from 407.5 to 651.9 nm and the entrapment efficiencies ranged from 56.02 to 65.23%. Interestingly, the 50% inhibitory concentration of the optimized formula had more than a 40-fold improved effect on the cell growth inhibition in comparison with its free counterpart. Furthermore, it was more selective against cancer cells than normal cells particularly in MCF-7 breast cancer cells.Conclusion: These data proved that nanoencapsulation of PE enhanced its anticancer efficacy. Therefore, our results suggested that a PE-loaded SLNs optimized-formula could be a promising chemotherapeutic agent. Keywords: pomegranate extract, solid lipid nanoparticles, Box–Behnken design, optimization, cancer cell linesBadawi NMTeaima MHEl-Say KMAttia DAEl-Nabarawi MAElmazar MMDove Medical PressarticlePomegranate extractSolid lipid nanoparticlesBox–Behnken designoptimizationcancer cell linesMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 13, Pp 1313-1326 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Pomegranate extract Solid lipid nanoparticles Box–Behnken design optimization cancer cell lines Medicine (General) R5-920
spellingShingle	Pomegranate extract Solid lipid nanoparticles Box–Behnken design optimization cancer cell lines Medicine (General) R5-920 Badawi NM Teaima MH El-Say KM Attia DA El-Nabarawi MA Elmazar MM Pomegranate extract-loaded solid lipid nanoparticles: design, optimization, and in vitro cytotoxicity study
description	Noha M Badawi,1 Mahmoud H Teaima,2 Khalid M El-Say,3 Dalia A Attia,1 Mohamed A El-Nabarawi,2 Mohey M Elmazar4 1Department of Pharmaceutics, Faculty of Pharmacy, The British University in Egypt, Cairo, Egypt; 2Department of Pharmaceutics and Industrial Pharmacy, Cairo University, Cairo, Egypt; 3Department of Pharmaceutics, King Abdulaziz University, Jeddah, Saudi Arabia; 4Department of Pharmacology, Faculty of Pharmacy, The British University in Egypt, Cairo, Egypt Background: Pomegranate extract (PE) is a natural product with potent antioxidant and anticancer activity because of its polyphenols content. The main purpose of this study was to maximize the PE chemotherapeutic efficacy by loading it in an optimized solid lipid nanoparticles (SLNs) formula.Materials and methods: The influence of independent variables, which were lipid concentration (X1), surfactant concentration (X2) and cosurfactant concentration (X3), on dependent ones, which were particle size (Y1), polydispersity index (Y2), zeta potential (Y3), entrapment efficiency (Y4) and cumulative % drug release (Y5), were studied and optimized using the Box–Behnken design. Fifteen formulations of PE-SLNs were prepared using hot homogenization followed by ultra-sonication technique. Response surface plots, Pareto charts and mathematical equations were produced to study the impact of independent variables on the dependent quality parameters. The anti-proliferative activity of the optimized formula was then evaluated in three different cancer cell lines, namely, MCF-7, PC-3 and HepG-2, in addition to one normal cell line, HFB-4.Results: The results demonstrated that the particle sizes ranged from 407.5 to 651.9 nm and the entrapment efficiencies ranged from 56.02 to 65.23%. Interestingly, the 50% inhibitory concentration of the optimized formula had more than a 40-fold improved effect on the cell growth inhibition in comparison with its free counterpart. Furthermore, it was more selective against cancer cells than normal cells particularly in MCF-7 breast cancer cells.Conclusion: These data proved that nanoencapsulation of PE enhanced its anticancer efficacy. Therefore, our results suggested that a PE-loaded SLNs optimized-formula could be a promising chemotherapeutic agent. Keywords: pomegranate extract, solid lipid nanoparticles, Box–Behnken design, optimization, cancer cell lines
format	article
author	Badawi NM Teaima MH El-Say KM Attia DA El-Nabarawi MA Elmazar MM
author_facet	Badawi NM Teaima MH El-Say KM Attia DA El-Nabarawi MA Elmazar MM
author_sort	Badawi NM
title	Pomegranate extract-loaded solid lipid nanoparticles: design, optimization, and in vitro cytotoxicity study
title_short	Pomegranate extract-loaded solid lipid nanoparticles: design, optimization, and in vitro cytotoxicity study
title_full	Pomegranate extract-loaded solid lipid nanoparticles: design, optimization, and in vitro cytotoxicity study
title_fullStr	Pomegranate extract-loaded solid lipid nanoparticles: design, optimization, and in vitro cytotoxicity study
title_full_unstemmed	Pomegranate extract-loaded solid lipid nanoparticles: design, optimization, and in vitro cytotoxicity study
title_sort	pomegranate extract-loaded solid lipid nanoparticles: design, optimization, and in vitro cytotoxicity study
publisher	Dove Medical Press
publishDate	2018
url	https://doaj.org/article/7c155d184abc457db8b1bf771731f732
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_version_	1718402228815921152

Pomegranate extract-loaded solid lipid nanoparticles: design, optimization, and in vitro cytotoxicity study

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