Effects of hMASP-2 on the formation of BCG infection-induced granuloma in the lungs of BALB/c mice

Abstract Tuberculosis, caused by Mycobacterium tuberculosis, affects the functions of the lung and causes high morbidity and mortality rates worldwide. MASP-2 is an executioner enzyme, which plays an essential role in the activation of lectin pathway. In our previous studies, the MASP-2 played a dua...

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Autores principales:	Xiaoying Xu, Xiaoling Lu, Xingfang Dong, Yanping Luo, Qian Wang, Xun Liu, Jie Fu, Yuan Zhang, Bingdong Zhu, Xingming Ma
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7c1627ec235349c68cda3d9447c01541
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spelling	oai:doaj.org-article:7c1627ec235349c68cda3d9447c015412021-12-02T16:06:23ZEffects of hMASP-2 on the formation of BCG infection-induced granuloma in the lungs of BALB/c mice10.1038/s41598-017-02374-z2045-2322https://doaj.org/article/7c1627ec235349c68cda3d9447c015412017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-02374-zhttps://doaj.org/toc/2045-2322Abstract Tuberculosis, caused by Mycobacterium tuberculosis, affects the functions of the lung and causes high morbidity and mortality rates worldwide. MASP-2 is an executioner enzyme, which plays an essential role in the activation of lectin pathway. In our previous studies, the MASP-2 played a dual role in promoting the progress of lesions in BCG-infected rabbit skin models. However, the really effects of MASP-2 on tuberculosis are unknown. The aim of this study was to investigate the effects of MASP-2 in granuloma formation with BCG-infected mice. Compared to the control group, rAd-hMASP-2 treated group showed increasing in survival rate of BCG-infected mice (P = 0.042), and decreasing of bacteria loads (P = 0.005) in the lung tissue. MASP-2 displayed a protective efficacy in BCG-infected mice, which promoted the activation and recruitment of macrophages and lymphocytes to the granuloma. Moreover, the data obtained from the ELISA and RT-PCR demonstrated that mRNA expression for IL-6, CCL12, CCL2 and cytokines of IFN-γ, TNF-α in lung were significantly elevated by treatment of rAd-hMASP-2. Those findings provided an evidence that MASP-2 may be as a newly immunomodulatory in targeting granuloma formation, which displayed a potential protective role in control of tuberculosis.Xiaoying XuXiaoling LuXingfang DongYanping LuoQian WangXun LiuJie FuYuan ZhangBingdong ZhuXingming MaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Xiaoying Xu Xiaoling Lu Xingfang Dong Yanping Luo Qian Wang Xun Liu Jie Fu Yuan Zhang Bingdong Zhu Xingming Ma Effects of hMASP-2 on the formation of BCG infection-induced granuloma in the lungs of BALB/c mice
description	Abstract Tuberculosis, caused by Mycobacterium tuberculosis, affects the functions of the lung and causes high morbidity and mortality rates worldwide. MASP-2 is an executioner enzyme, which plays an essential role in the activation of lectin pathway. In our previous studies, the MASP-2 played a dual role in promoting the progress of lesions in BCG-infected rabbit skin models. However, the really effects of MASP-2 on tuberculosis are unknown. The aim of this study was to investigate the effects of MASP-2 in granuloma formation with BCG-infected mice. Compared to the control group, rAd-hMASP-2 treated group showed increasing in survival rate of BCG-infected mice (P = 0.042), and decreasing of bacteria loads (P = 0.005) in the lung tissue. MASP-2 displayed a protective efficacy in BCG-infected mice, which promoted the activation and recruitment of macrophages and lymphocytes to the granuloma. Moreover, the data obtained from the ELISA and RT-PCR demonstrated that mRNA expression for IL-6, CCL12, CCL2 and cytokines of IFN-γ, TNF-α in lung were significantly elevated by treatment of rAd-hMASP-2. Those findings provided an evidence that MASP-2 may be as a newly immunomodulatory in targeting granuloma formation, which displayed a potential protective role in control of tuberculosis.
format	article
author	Xiaoying Xu Xiaoling Lu Xingfang Dong Yanping Luo Qian Wang Xun Liu Jie Fu Yuan Zhang Bingdong Zhu Xingming Ma
author_facet	Xiaoying Xu Xiaoling Lu Xingfang Dong Yanping Luo Qian Wang Xun Liu Jie Fu Yuan Zhang Bingdong Zhu Xingming Ma
author_sort	Xiaoying Xu
title	Effects of hMASP-2 on the formation of BCG infection-induced granuloma in the lungs of BALB/c mice
title_short	Effects of hMASP-2 on the formation of BCG infection-induced granuloma in the lungs of BALB/c mice
title_full	Effects of hMASP-2 on the formation of BCG infection-induced granuloma in the lungs of BALB/c mice
title_fullStr	Effects of hMASP-2 on the formation of BCG infection-induced granuloma in the lungs of BALB/c mice
title_full_unstemmed	Effects of hMASP-2 on the formation of BCG infection-induced granuloma in the lungs of BALB/c mice
title_sort	effects of hmasp-2 on the formation of bcg infection-induced granuloma in the lungs of balb/c mice
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/7c1627ec235349c68cda3d9447c01541
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Effects of hMASP-2 on the formation of BCG infection-induced granuloma in the lungs of BALB/c mice

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