Silicon Nanowire Field-Effect Transistor as Label-Free Detection of Hepatitis B Virus Proteins with Opposite Net Charges

The prevalence of hepatitis B virus (HBV) is a global healthcare threat, particularly chronic hepatitis B (CHB) that might lead to hepatocellular carcinoma (HCC) should not be neglected. Although many types of HBV diagnosis detection methods are available, some technical challenges, such as the high...

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Autores principales:	Suh Kuan Yong, Shang-Kai Shen, Chia-Wei Chiang, Ying-Ya Weng, Ming-Pei Lu, Yuh-Shyong Yang
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	hepatitis B virus hepatitis B virus surface antigen (HBsAg) hepatitis B virus X protein (HBx) chronic hepatitis B (CHB) hepatocellular carcinoma (HCC) biomarker Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/7c1f0187e6204a90b9fda2ae69706a9c
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spelling	oai:doaj.org-article:7c1f0187e6204a90b9fda2ae69706a9c2021-11-25T16:55:27ZSilicon Nanowire Field-Effect Transistor as Label-Free Detection of Hepatitis B Virus Proteins with Opposite Net Charges10.3390/bios111104422079-6374https://doaj.org/article/7c1f0187e6204a90b9fda2ae69706a9c2021-11-01T00:00:00Zhttps://www.mdpi.com/2079-6374/11/11/442https://doaj.org/toc/2079-6374The prevalence of hepatitis B virus (HBV) is a global healthcare threat, particularly chronic hepatitis B (CHB) that might lead to hepatocellular carcinoma (HCC) should not be neglected. Although many types of HBV diagnosis detection methods are available, some technical challenges, such as the high cost or lack of practical feasibility, need to be overcome. In this study, the polycrystalline silicon nanowire field-effect transistors (pSiNWFETs) were fabricated through commercial process technology and then chemically functionalized for sensing hepatitis B virus surface antigen (HBsAg) and hepatitis B virus X protein (HBx) at the femto-molar level. These two proteins have been suggested to be related to the HCC development, while the former is also the hallmark for HBV diagnosis, and the latter is an RNA-binding protein. Interestingly, these two proteins carried opposite net charges, which could serve as complementary candidates for evaluating the charge-based sensing mechanism in the pSiNWFET. The measurements on the threshold voltage shifts of pSiNWFETs showed a consistent correspondence to the polarity of the charges on the proteins studied. We believe that this report can pave the way towards developing an approachable tool for biomedical applications.Suh Kuan YongShang-Kai ShenChia-Wei ChiangYing-Ya WengMing-Pei LuYuh-Shyong YangMDPI AGarticlehepatitis B virushepatitis B virus surface antigen (HBsAg)hepatitis B virus X protein (HBx)chronic hepatitis B (CHB)hepatocellular carcinoma (HCC)biomarkerBiotechnologyTP248.13-248.65ENBiosensors, Vol 11, Iss 442, p 442 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	hepatitis B virus hepatitis B virus surface antigen (HBsAg) hepatitis B virus X protein (HBx) chronic hepatitis B (CHB) hepatocellular carcinoma (HCC) biomarker Biotechnology TP248.13-248.65
spellingShingle	hepatitis B virus hepatitis B virus surface antigen (HBsAg) hepatitis B virus X protein (HBx) chronic hepatitis B (CHB) hepatocellular carcinoma (HCC) biomarker Biotechnology TP248.13-248.65 Suh Kuan Yong Shang-Kai Shen Chia-Wei Chiang Ying-Ya Weng Ming-Pei Lu Yuh-Shyong Yang Silicon Nanowire Field-Effect Transistor as Label-Free Detection of Hepatitis B Virus Proteins with Opposite Net Charges
description	The prevalence of hepatitis B virus (HBV) is a global healthcare threat, particularly chronic hepatitis B (CHB) that might lead to hepatocellular carcinoma (HCC) should not be neglected. Although many types of HBV diagnosis detection methods are available, some technical challenges, such as the high cost or lack of practical feasibility, need to be overcome. In this study, the polycrystalline silicon nanowire field-effect transistors (pSiNWFETs) were fabricated through commercial process technology and then chemically functionalized for sensing hepatitis B virus surface antigen (HBsAg) and hepatitis B virus X protein (HBx) at the femto-molar level. These two proteins have been suggested to be related to the HCC development, while the former is also the hallmark for HBV diagnosis, and the latter is an RNA-binding protein. Interestingly, these two proteins carried opposite net charges, which could serve as complementary candidates for evaluating the charge-based sensing mechanism in the pSiNWFET. The measurements on the threshold voltage shifts of pSiNWFETs showed a consistent correspondence to the polarity of the charges on the proteins studied. We believe that this report can pave the way towards developing an approachable tool for biomedical applications.
format	article
author	Suh Kuan Yong Shang-Kai Shen Chia-Wei Chiang Ying-Ya Weng Ming-Pei Lu Yuh-Shyong Yang
author_facet	Suh Kuan Yong Shang-Kai Shen Chia-Wei Chiang Ying-Ya Weng Ming-Pei Lu Yuh-Shyong Yang
author_sort	Suh Kuan Yong
title	Silicon Nanowire Field-Effect Transistor as Label-Free Detection of Hepatitis B Virus Proteins with Opposite Net Charges
title_short	Silicon Nanowire Field-Effect Transistor as Label-Free Detection of Hepatitis B Virus Proteins with Opposite Net Charges
title_full	Silicon Nanowire Field-Effect Transistor as Label-Free Detection of Hepatitis B Virus Proteins with Opposite Net Charges
title_fullStr	Silicon Nanowire Field-Effect Transistor as Label-Free Detection of Hepatitis B Virus Proteins with Opposite Net Charges
title_full_unstemmed	Silicon Nanowire Field-Effect Transistor as Label-Free Detection of Hepatitis B Virus Proteins with Opposite Net Charges
title_sort	silicon nanowire field-effect transistor as label-free detection of hepatitis b virus proteins with opposite net charges
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/7c1f0187e6204a90b9fda2ae69706a9c
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Silicon Nanowire Field-Effect Transistor as Label-Free Detection of Hepatitis B Virus Proteins with Opposite Net Charges

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