Genome Characterization of Bird-Related Rhabdoviruses Circulating in Africa

<i>Rhabdoviridae</i> is the most diverse family of the negative, single-stranded RNA viruses, which includes 40 ecologically different genera that infect plants, insects, reptiles, fishes, and mammals, including humans, and birds. To date, only a few bird-related rhabdoviruses among the...

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Autores principales:	Dong-Sheng Luo, Zhi-Jian Zhou, Xing-Yi Ge, Hervé Bourhy, Zheng-Li Shi, Marc Grandadam, Laurent Dacheux
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	rhabdovirus <i>Sunrhavirus</i> bird genome Africa NGS Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/7c278cde6e994e598632827d3ca62196
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id	oai:doaj.org-article:7c278cde6e994e598632827d3ca62196
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spelling	oai:doaj.org-article:7c278cde6e994e598632827d3ca621962021-11-25T19:12:59ZGenome Characterization of Bird-Related Rhabdoviruses Circulating in Africa10.3390/v131121681999-4915https://doaj.org/article/7c278cde6e994e598632827d3ca621962021-10-01T00:00:00Zhttps://www.mdpi.com/1999-4915/13/11/2168https://doaj.org/toc/1999-4915<i>Rhabdoviridae</i> is the most diverse family of the negative, single-stranded RNA viruses, which includes 40 ecologically different genera that infect plants, insects, reptiles, fishes, and mammals, including humans, and birds. To date, only a few bird-related rhabdoviruses among the genera <i>Sunrhavirus</i>, <i>Hapavirus</i>, and <i>Tupavirus</i> have been described and analyzed at the molecular level. In this study, we characterized seven additional and previously unclassified rhabdoviruses, which were isolated from various bird species collected in Africa during the 1960s and 1970s. Based on the analysis of their genome sequences obtained by next generation sequencing, we observed a classical genomic structure, with the presence of the five canonical rhabdovirus genes, i.e., nucleoprotein (N), phosphoprotein (P), matrix protein (M), glycoprotein (G), and polymerase (L). In addition, different additional open reading frames which code putative proteins of unknown function were identified, with the common presence of the C and the SH proteins, within the P gene and between the M and G genes, respectively. Genetic comparisons and phylogenetic analysis demonstrated that these seven bird-related rhabdoviruses could be considered as putative new species within the genus <i>Sunrhavirus</i>, where they clustered into a single group (named Clade III), a companion to two other groups that encompass mainly insect-related viruses. The results of this study shed light on the high diversity of the rhabdoviruses circulating in birds, mainly in Africa. Their close relationship with other insect-related sunrhaviruses raise questions about their potential role and impact as arboviruses that affect bird communities.Dong-Sheng LuoZhi-Jian ZhouXing-Yi GeHervé BourhyZheng-Li ShiMarc GrandadamLaurent DacheuxMDPI AGarticlerhabdovirus<i>Sunrhavirus</i>birdgenomeAfricaNGSMicrobiologyQR1-502ENViruses, Vol 13, Iss 2168, p 2168 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	rhabdovirus <i>Sunrhavirus</i> bird genome Africa NGS Microbiology QR1-502
spellingShingle	rhabdovirus <i>Sunrhavirus</i> bird genome Africa NGS Microbiology QR1-502 Dong-Sheng Luo Zhi-Jian Zhou Xing-Yi Ge Hervé Bourhy Zheng-Li Shi Marc Grandadam Laurent Dacheux Genome Characterization of Bird-Related Rhabdoviruses Circulating in Africa
description	<i>Rhabdoviridae</i> is the most diverse family of the negative, single-stranded RNA viruses, which includes 40 ecologically different genera that infect plants, insects, reptiles, fishes, and mammals, including humans, and birds. To date, only a few bird-related rhabdoviruses among the genera <i>Sunrhavirus</i>, <i>Hapavirus</i>, and <i>Tupavirus</i> have been described and analyzed at the molecular level. In this study, we characterized seven additional and previously unclassified rhabdoviruses, which were isolated from various bird species collected in Africa during the 1960s and 1970s. Based on the analysis of their genome sequences obtained by next generation sequencing, we observed a classical genomic structure, with the presence of the five canonical rhabdovirus genes, i.e., nucleoprotein (N), phosphoprotein (P), matrix protein (M), glycoprotein (G), and polymerase (L). In addition, different additional open reading frames which code putative proteins of unknown function were identified, with the common presence of the C and the SH proteins, within the P gene and between the M and G genes, respectively. Genetic comparisons and phylogenetic analysis demonstrated that these seven bird-related rhabdoviruses could be considered as putative new species within the genus <i>Sunrhavirus</i>, where they clustered into a single group (named Clade III), a companion to two other groups that encompass mainly insect-related viruses. The results of this study shed light on the high diversity of the rhabdoviruses circulating in birds, mainly in Africa. Their close relationship with other insect-related sunrhaviruses raise questions about their potential role and impact as arboviruses that affect bird communities.
format	article
author	Dong-Sheng Luo Zhi-Jian Zhou Xing-Yi Ge Hervé Bourhy Zheng-Li Shi Marc Grandadam Laurent Dacheux
author_facet	Dong-Sheng Luo Zhi-Jian Zhou Xing-Yi Ge Hervé Bourhy Zheng-Li Shi Marc Grandadam Laurent Dacheux
author_sort	Dong-Sheng Luo
title	Genome Characterization of Bird-Related Rhabdoviruses Circulating in Africa
title_short	Genome Characterization of Bird-Related Rhabdoviruses Circulating in Africa
title_full	Genome Characterization of Bird-Related Rhabdoviruses Circulating in Africa
title_fullStr	Genome Characterization of Bird-Related Rhabdoviruses Circulating in Africa
title_full_unstemmed	Genome Characterization of Bird-Related Rhabdoviruses Circulating in Africa
title_sort	genome characterization of bird-related rhabdoviruses circulating in africa
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/7c278cde6e994e598632827d3ca62196
work_keys_str_mv	AT dongshengluo genomecharacterizationofbirdrelatedrhabdovirusescirculatinginafrica AT zhijianzhou genomecharacterizationofbirdrelatedrhabdovirusescirculatinginafrica AT xingyige genomecharacterizationofbirdrelatedrhabdovirusescirculatinginafrica AT hervebourhy genomecharacterizationofbirdrelatedrhabdovirusescirculatinginafrica AT zhenglishi genomecharacterizationofbirdrelatedrhabdovirusescirculatinginafrica AT marcgrandadam genomecharacterizationofbirdrelatedrhabdovirusescirculatinginafrica AT laurentdacheux genomecharacterizationofbirdrelatedrhabdovirusescirculatinginafrica
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Genome Characterization of Bird-Related Rhabdoviruses Circulating in Africa

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