The OXI1 kinase pathway mediates Piriformospora indica-induced growth promotion in Arabidopsis.

Piriformospora indica is an endophytic fungus that colonizes roots of many plant species and promotes growth and resistance to certain plant pathogens. Despite its potential use in agriculture, little is known on the molecular basis of this beneficial plant-fungal interaction. In a genetic screen fo...

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Autores principales:	Iris Camehl, Corinna Drzewiecki, Jyothilakshmi Vadassery, Bationa Shahollari, Irena Sherameti, Celine Forzani, Teun Munnik, Heribert Hirt, Ralf Oelmüller
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/7c2b992e627849a9a6ee6317b66b9849
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spelling	oai:doaj.org-article:7c2b992e627849a9a6ee6317b66b98492021-11-18T06:03:23ZThe OXI1 kinase pathway mediates Piriformospora indica-induced growth promotion in Arabidopsis.1553-73661553-737410.1371/journal.ppat.1002051https://doaj.org/article/7c2b992e627849a9a6ee6317b66b98492011-05-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21625539/?tool=EBIhttps://doaj.org/toc/1553-7366https://doaj.org/toc/1553-7374Piriformospora indica is an endophytic fungus that colonizes roots of many plant species and promotes growth and resistance to certain plant pathogens. Despite its potential use in agriculture, little is known on the molecular basis of this beneficial plant-fungal interaction. In a genetic screen for plants, which do not show a P. indica- induced growth response, we isolated an Arabidopsis mutant in the OXI1 (Oxidative Signal Inducible1) gene. OXI1 has been characterized as a protein kinase which plays a role in pathogen response and is regulated by H₂O₂ and PDK1 (3-PHOSPHOINOSITIDE-DEPENDENT PROTEIN KINASE1). A genetic analysis showed that double mutants of the two closely related PDK1.1 and PDK1.2 genes are defective in the growth response to P. indica. While OXI1 and PDK1 gene expression is upregulated in P. indica-colonized roots, defense genes are downregulated, indicating that the fungus suppresses plant defense reactions. PDK1 is activated by phosphatidic acid (PA) and P. indica triggers PA synthesis in Arabidopsis plants. Under beneficial co-cultivation conditions, H₂O₂ formation is even reduced by the fungus. Importantly, phospholipase D (PLD)α1 or PLDδ mutants, which are impaired in PA synthesis do not show growth promotion in response to fungal infection. These data establish that the P. indica-stimulated growth response is mediated by a pathway consisting of the PLD-PDK1-OXI1 cascade.Iris CamehlCorinna DrzewieckiJyothilakshmi VadasseryBationa ShahollariIrena SherametiCeline ForzaniTeun MunnikHeribert HirtRalf OelmüllerPublic Library of Science (PLoS)articleImmunologic diseases. AllergyRC581-607Biology (General)QH301-705.5ENPLoS Pathogens, Vol 7, Iss 5, p e1002051 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
spellingShingle	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 Iris Camehl Corinna Drzewiecki Jyothilakshmi Vadassery Bationa Shahollari Irena Sherameti Celine Forzani Teun Munnik Heribert Hirt Ralf Oelmüller The OXI1 kinase pathway mediates Piriformospora indica-induced growth promotion in Arabidopsis.
description	Piriformospora indica is an endophytic fungus that colonizes roots of many plant species and promotes growth and resistance to certain plant pathogens. Despite its potential use in agriculture, little is known on the molecular basis of this beneficial plant-fungal interaction. In a genetic screen for plants, which do not show a P. indica- induced growth response, we isolated an Arabidopsis mutant in the OXI1 (Oxidative Signal Inducible1) gene. OXI1 has been characterized as a protein kinase which plays a role in pathogen response and is regulated by H₂O₂ and PDK1 (3-PHOSPHOINOSITIDE-DEPENDENT PROTEIN KINASE1). A genetic analysis showed that double mutants of the two closely related PDK1.1 and PDK1.2 genes are defective in the growth response to P. indica. While OXI1 and PDK1 gene expression is upregulated in P. indica-colonized roots, defense genes are downregulated, indicating that the fungus suppresses plant defense reactions. PDK1 is activated by phosphatidic acid (PA) and P. indica triggers PA synthesis in Arabidopsis plants. Under beneficial co-cultivation conditions, H₂O₂ formation is even reduced by the fungus. Importantly, phospholipase D (PLD)α1 or PLDδ mutants, which are impaired in PA synthesis do not show growth promotion in response to fungal infection. These data establish that the P. indica-stimulated growth response is mediated by a pathway consisting of the PLD-PDK1-OXI1 cascade.
format	article
author	Iris Camehl Corinna Drzewiecki Jyothilakshmi Vadassery Bationa Shahollari Irena Sherameti Celine Forzani Teun Munnik Heribert Hirt Ralf Oelmüller
author_facet	Iris Camehl Corinna Drzewiecki Jyothilakshmi Vadassery Bationa Shahollari Irena Sherameti Celine Forzani Teun Munnik Heribert Hirt Ralf Oelmüller
author_sort	Iris Camehl
title	The OXI1 kinase pathway mediates Piriformospora indica-induced growth promotion in Arabidopsis.
title_short	The OXI1 kinase pathway mediates Piriformospora indica-induced growth promotion in Arabidopsis.
title_full	The OXI1 kinase pathway mediates Piriformospora indica-induced growth promotion in Arabidopsis.
title_fullStr	The OXI1 kinase pathway mediates Piriformospora indica-induced growth promotion in Arabidopsis.
title_full_unstemmed	The OXI1 kinase pathway mediates Piriformospora indica-induced growth promotion in Arabidopsis.
title_sort	oxi1 kinase pathway mediates piriformospora indica-induced growth promotion in arabidopsis.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/7c2b992e627849a9a6ee6317b66b9849
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The OXI1 kinase pathway mediates Piriformospora indica-induced growth promotion in Arabidopsis.

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