In Vitro Propagation of Easter Island Curcuma longa from Rhizome Explants Using Temporary Immersion System

Curcuma longa (C. longa) is widely known for its medicinal properties. However, the potential overexploitation of this plant raises doubts about its long-term survival on Rapa Nui. Micropropagation using a temporary immersion system (TIS) could be the basis for...

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Autores principales:	María José Marchant, Paula Molina, Miriam Montecinos, Leda Guzmán, Cristobal Balada, Claudia Fassio, Mónica Castro
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	turmeric micropropagation LED light conservation Rapa Nui automatization Agriculture S
Acceso en línea:	https://doaj.org/article/7c2d20e2ae3042aa823587efff92891b
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spelling	oai:doaj.org-article:7c2d20e2ae3042aa823587efff92891b2021-11-25T16:03:23ZIn Vitro Propagation of Easter Island <i>Curcuma longa</i> from Rhizome Explants Using Temporary Immersion System10.3390/agronomy111121212073-4395https://doaj.org/article/7c2d20e2ae3042aa823587efff92891b2021-10-01T00:00:00Zhttps://www.mdpi.com/2073-4395/11/11/2121https://doaj.org/toc/2073-4395<i>Curcuma longa</i> (<i>C. longa</i>) is widely known for its medicinal properties. However, the potential overexploitation of this plant raises doubts about its long-term survival on Rapa Nui. Micropropagation using a temporary immersion system (TIS) could be the basis for developing a cost-effective and highly productive method of large-scale cultivation of this plant. Our objective was to develop and refine the in vitro multiplication system for mass propagation of <i>C. longa,</i> and thus help restore the fragile ecosystem of Rapa Nui. Three parameters were evaluated: number of explants per flask, flask capacity, and LEDs spectrum. For each parameter evaluated, four aspects were analyzed: fresh weight per plant, number of shoots, percentage of non-sprouting explants, and the proliferation rate. The use of 30 explants per two-liter flask results in more plants with high fresh biomass than other configurations. In addition, LEDs with a red:blue ratio of 2:1 provided the best lighting conditions for in vitro propagation and positively affected <i>C. longa</i> proliferation and rooting. Therefore, our results show that 30 explants per two-liter flask and an LED source with a red:blue ratio of 2:1 allow a higher number of <i>C. longa</i> plants to be obtained using TIS.María José MarchantPaula MolinaMiriam MontecinosLeda GuzmánCristobal BaladaClaudia FassioMónica CastroMDPI AGarticleturmericmicropropagationLED lightconservationRapa NuiautomatizationAgricultureSENAgronomy, Vol 11, Iss 2121, p 2121 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	turmeric micropropagation LED light conservation Rapa Nui automatization Agriculture S
spellingShingle	turmeric micropropagation LED light conservation Rapa Nui automatization Agriculture S María José Marchant Paula Molina Miriam Montecinos Leda Guzmán Cristobal Balada Claudia Fassio Mónica Castro In Vitro Propagation of Easter Island <i>Curcuma longa</i> from Rhizome Explants Using Temporary Immersion System
description	<i>Curcuma longa</i> (<i>C. longa</i>) is widely known for its medicinal properties. However, the potential overexploitation of this plant raises doubts about its long-term survival on Rapa Nui. Micropropagation using a temporary immersion system (TIS) could be the basis for developing a cost-effective and highly productive method of large-scale cultivation of this plant. Our objective was to develop and refine the in vitro multiplication system for mass propagation of <i>C. longa,</i> and thus help restore the fragile ecosystem of Rapa Nui. Three parameters were evaluated: number of explants per flask, flask capacity, and LEDs spectrum. For each parameter evaluated, four aspects were analyzed: fresh weight per plant, number of shoots, percentage of non-sprouting explants, and the proliferation rate. The use of 30 explants per two-liter flask results in more plants with high fresh biomass than other configurations. In addition, LEDs with a red:blue ratio of 2:1 provided the best lighting conditions for in vitro propagation and positively affected <i>C. longa</i> proliferation and rooting. Therefore, our results show that 30 explants per two-liter flask and an LED source with a red:blue ratio of 2:1 allow a higher number of <i>C. longa</i> plants to be obtained using TIS.
format	article
author	María José Marchant Paula Molina Miriam Montecinos Leda Guzmán Cristobal Balada Claudia Fassio Mónica Castro
author_facet	María José Marchant Paula Molina Miriam Montecinos Leda Guzmán Cristobal Balada Claudia Fassio Mónica Castro
author_sort	María José Marchant
title	In Vitro Propagation of Easter Island <i>Curcuma longa</i> from Rhizome Explants Using Temporary Immersion System
title_short	In Vitro Propagation of Easter Island <i>Curcuma longa</i> from Rhizome Explants Using Temporary Immersion System
title_full	In Vitro Propagation of Easter Island <i>Curcuma longa</i> from Rhizome Explants Using Temporary Immersion System
title_fullStr	In Vitro Propagation of Easter Island <i>Curcuma longa</i> from Rhizome Explants Using Temporary Immersion System
title_full_unstemmed	In Vitro Propagation of Easter Island <i>Curcuma longa</i> from Rhizome Explants Using Temporary Immersion System
title_sort	in vitro propagation of easter island <i>curcuma longa</i> from rhizome explants using temporary immersion system
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/7c2d20e2ae3042aa823587efff92891b
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In Vitro Propagation of Easter Island <i>Curcuma longa</i> from Rhizome Explants Using Temporary Immersion System

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