Immunostimulatory activity of exopolysaccharides from probiotic Lactobacillus casei WXD030 strain as a novel adjuvant in vitro and in vivo

Exopolysaccharides act as mediators of cross-talk between probiotics and the host. Here, we found that EPS derived from probiotic Lactobacillus casei WXD030 strain (L-EPS) could modulate immune responses in vitro and in vivo. L-EPS could significantly enhance the proliferation and phagocytic activit...

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Autores principales:	Lei Xiu, Haochi Zhang, Zhongpeng Hu, Yanchen Liang, Shuai Guo, Ming Yang, Ruiping Du, Xiao Wang
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2018
Materias:	exopolysaccharides lactobacillus casei adjuvant immune responses Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/7c3d42cc2c04429bac7b4c46a1104ec4
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spelling	oai:doaj.org-article:7c3d42cc2c04429bac7b4c46a1104ec42021-11-26T11:19:47ZImmunostimulatory activity of exopolysaccharides from probiotic Lactobacillus casei WXD030 strain as a novel adjuvant in vitro and in vivo0954-01051465-344310.1080/09540105.2018.1513994https://doaj.org/article/7c3d42cc2c04429bac7b4c46a1104ec42018-01-01T00:00:00Zhttp://dx.doi.org/10.1080/09540105.2018.1513994https://doaj.org/toc/0954-0105https://doaj.org/toc/1465-3443Exopolysaccharides act as mediators of cross-talk between probiotics and the host. Here, we found that EPS derived from probiotic Lactobacillus casei WXD030 strain (L-EPS) could modulate immune responses in vitro and in vivo. L-EPS could significantly enhance the proliferation and phagocytic activity as well as induce the production of NO, TNF-α, IL-1β and IL-6 in RAW264.7 cells. Furthermore, L-EPS could induce the maturation of BMDCs. In addition, L-EPS could largely increase the titres of OVA-specific antibodies and markedly enhanced T cell proliferation. Notably, L-EPS also increased expression of IL-4 and INF-γ expression in CD4+T cells. Consistently, when used as an adjuvant in vivo with the foot-and-mouth disease vaccine, L-EPS largely enhanced the FMDV-specific antibody production. Collectively, these results suggested that L-EPS derived from probiotic L. casei strain had adjuvant activity, which may be a safe and efficacious adjuvant candidate suitable for a wide spectrum of prophylactic and therapeutic vaccines.Lei XiuHaochi ZhangZhongpeng HuYanchen LiangShuai GuoMing YangRuiping DuXiao WangTaylor & Francis Grouparticleexopolysaccharideslactobacillus caseiadjuvantimmune responsesAgriculture (General)S1-972Immunologic diseases. AllergyRC581-607ENFood and Agricultural Immunology, Vol 29, Iss 1, Pp 1086-1105 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	exopolysaccharides lactobacillus casei adjuvant immune responses Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607
spellingShingle	exopolysaccharides lactobacillus casei adjuvant immune responses Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607 Lei Xiu Haochi Zhang Zhongpeng Hu Yanchen Liang Shuai Guo Ming Yang Ruiping Du Xiao Wang Immunostimulatory activity of exopolysaccharides from probiotic Lactobacillus casei WXD030 strain as a novel adjuvant in vitro and in vivo
description	Exopolysaccharides act as mediators of cross-talk between probiotics and the host. Here, we found that EPS derived from probiotic Lactobacillus casei WXD030 strain (L-EPS) could modulate immune responses in vitro and in vivo. L-EPS could significantly enhance the proliferation and phagocytic activity as well as induce the production of NO, TNF-α, IL-1β and IL-6 in RAW264.7 cells. Furthermore, L-EPS could induce the maturation of BMDCs. In addition, L-EPS could largely increase the titres of OVA-specific antibodies and markedly enhanced T cell proliferation. Notably, L-EPS also increased expression of IL-4 and INF-γ expression in CD4+T cells. Consistently, when used as an adjuvant in vivo with the foot-and-mouth disease vaccine, L-EPS largely enhanced the FMDV-specific antibody production. Collectively, these results suggested that L-EPS derived from probiotic L. casei strain had adjuvant activity, which may be a safe and efficacious adjuvant candidate suitable for a wide spectrum of prophylactic and therapeutic vaccines.
format	article
author	Lei Xiu Haochi Zhang Zhongpeng Hu Yanchen Liang Shuai Guo Ming Yang Ruiping Du Xiao Wang
author_facet	Lei Xiu Haochi Zhang Zhongpeng Hu Yanchen Liang Shuai Guo Ming Yang Ruiping Du Xiao Wang
author_sort	Lei Xiu
title	Immunostimulatory activity of exopolysaccharides from probiotic Lactobacillus casei WXD030 strain as a novel adjuvant in vitro and in vivo
title_short	Immunostimulatory activity of exopolysaccharides from probiotic Lactobacillus casei WXD030 strain as a novel adjuvant in vitro and in vivo
title_full	Immunostimulatory activity of exopolysaccharides from probiotic Lactobacillus casei WXD030 strain as a novel adjuvant in vitro and in vivo
title_fullStr	Immunostimulatory activity of exopolysaccharides from probiotic Lactobacillus casei WXD030 strain as a novel adjuvant in vitro and in vivo
title_full_unstemmed	Immunostimulatory activity of exopolysaccharides from probiotic Lactobacillus casei WXD030 strain as a novel adjuvant in vitro and in vivo
title_sort	immunostimulatory activity of exopolysaccharides from probiotic lactobacillus casei wxd030 strain as a novel adjuvant in vitro and in vivo
publisher	Taylor & Francis Group
publishDate	2018
url	https://doaj.org/article/7c3d42cc2c04429bac7b4c46a1104ec4
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Immunostimulatory activity of exopolysaccharides from probiotic Lactobacillus casei WXD030 strain as a novel adjuvant in vitro and in vivo

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