Roles of Exogenous α-Lipoic Acid and Cysteine in Mitigation of Drought Stress and Restoration of Grain Quality in Wheat

Cysteine (Cys) and α-lipoic acid (ALA) are naturally occurring antioxidants (sulfur-containing compounds) that can protect plants against a wide spectrum of environmental stresses. However, up to now, there are no conclusive data on their integrative roles in mitigation of drought stress in wheat pl...

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Autores principales:	Amr Elkelish, Mohamed M. El-Mogy, Gniewko Niedbała, Magdalena Piekutowska, Mohamed A. M. Atia, Maha M. A. Hamada, Mostafa Shahin, Soumya Mukherjee, Ahmed Abou El-Yazied, Mohamed Shebl, Mohammad Shah Jahan, Ali Osman, Hany G. Abd El-Gawad, Hatem Ashour, Reham Farag, Samy Selim, Mohamed F. M. Ibrahim
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	wheat water stress antioxidant capacity grain quality alveographic parameters alpha-lipoic acid Botany QK1-989
Acceso en línea:	https://doaj.org/article/7c3eed0308884de08f93bf13f13e71dd
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spelling	oai:doaj.org-article:7c3eed0308884de08f93bf13f13e71dd2021-11-25T18:45:17ZRoles of Exogenous α-Lipoic Acid and Cysteine in Mitigation of Drought Stress and Restoration of Grain Quality in Wheat10.3390/plants101123182223-7747https://doaj.org/article/7c3eed0308884de08f93bf13f13e71dd2021-10-01T00:00:00Zhttps://www.mdpi.com/2223-7747/10/11/2318https://doaj.org/toc/2223-7747Cysteine (Cys) and α-lipoic acid (ALA) are naturally occurring antioxidants (sulfur-containing compounds) that can protect plants against a wide spectrum of environmental stresses. However, up to now, there are no conclusive data on their integrative roles in mitigation of drought stress in wheat plants. Here, we studied the influence of ALA at 0.02 mM (grain dipping pre-cultivation treatment) and Cys (25 and 50 ppm as a foliar application) under well watered and deficit irrigation (100% and 70% of recommended dose). The results showed that deficit irrigation markedly caused obvious cellular oxidative damage as indicated by elevating the malondialdehyde (MDA) and hydrogen peroxide content (H<sub>2</sub>O<sub>2</sub>). Moreover, water stressed plants exhibited multiple changes in physiological metabolism, which affected the quantitative and qualitative variables of grain yield. The enzymatic antioxidants, including superoxide dismutase (SOD), ascorbate peroxidase (APX), catalase (CAT) and peroxidase (POX) were improved by Cys application. SOD and APX had the same response when treated with ALA, but CAT and POX did not. Moreover, both studied molecules stimulated chlorophyll (Chl) and osmolytes’ biosynthesis. In contrast, the Chl a/b ratio was decreased, while flavonoids were not affected by either of the examined molecules. Interestingly, all above-mentioned changes were associated with an improvement in the scavenging capacity of reactive oxygen species (ROS), leaf relative water content (RWC), grain number, total grain yield, weight of 1000 kernels, gluten index, falling number, and alveographic parameters (P, W, and P/L values). Furthermore, heatmap plot analysis revealed several significant correlations between different studied parameters, which may explore the importance of applied Cys and ALA as effective compounds in wheat cultivation under water deficit conditions.Amr ElkelishMohamed M. El-MogyGniewko NiedbałaMagdalena PiekutowskaMohamed A. M. AtiaMaha M. A. HamadaMostafa ShahinSoumya MukherjeeAhmed Abou El-YaziedMohamed SheblMohammad Shah JahanAli OsmanHany G. Abd El-GawadHatem AshourReham FaragSamy SelimMohamed F. M. IbrahimMDPI AGarticlewheatwater stressantioxidant capacitygrain qualityalveographic parametersalpha-lipoic acidBotanyQK1-989ENPlants, Vol 10, Iss 2318, p 2318 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	wheat water stress antioxidant capacity grain quality alveographic parameters alpha-lipoic acid Botany QK1-989
spellingShingle	wheat water stress antioxidant capacity grain quality alveographic parameters alpha-lipoic acid Botany QK1-989 Amr Elkelish Mohamed M. El-Mogy Gniewko Niedbała Magdalena Piekutowska Mohamed A. M. Atia Maha M. A. Hamada Mostafa Shahin Soumya Mukherjee Ahmed Abou El-Yazied Mohamed Shebl Mohammad Shah Jahan Ali Osman Hany G. Abd El-Gawad Hatem Ashour Reham Farag Samy Selim Mohamed F. M. Ibrahim Roles of Exogenous α-Lipoic Acid and Cysteine in Mitigation of Drought Stress and Restoration of Grain Quality in Wheat
description	Cysteine (Cys) and α-lipoic acid (ALA) are naturally occurring antioxidants (sulfur-containing compounds) that can protect plants against a wide spectrum of environmental stresses. However, up to now, there are no conclusive data on their integrative roles in mitigation of drought stress in wheat plants. Here, we studied the influence of ALA at 0.02 mM (grain dipping pre-cultivation treatment) and Cys (25 and 50 ppm as a foliar application) under well watered and deficit irrigation (100% and 70% of recommended dose). The results showed that deficit irrigation markedly caused obvious cellular oxidative damage as indicated by elevating the malondialdehyde (MDA) and hydrogen peroxide content (H<sub>2</sub>O<sub>2</sub>). Moreover, water stressed plants exhibited multiple changes in physiological metabolism, which affected the quantitative and qualitative variables of grain yield. The enzymatic antioxidants, including superoxide dismutase (SOD), ascorbate peroxidase (APX), catalase (CAT) and peroxidase (POX) were improved by Cys application. SOD and APX had the same response when treated with ALA, but CAT and POX did not. Moreover, both studied molecules stimulated chlorophyll (Chl) and osmolytes’ biosynthesis. In contrast, the Chl a/b ratio was decreased, while flavonoids were not affected by either of the examined molecules. Interestingly, all above-mentioned changes were associated with an improvement in the scavenging capacity of reactive oxygen species (ROS), leaf relative water content (RWC), grain number, total grain yield, weight of 1000 kernels, gluten index, falling number, and alveographic parameters (P, W, and P/L values). Furthermore, heatmap plot analysis revealed several significant correlations between different studied parameters, which may explore the importance of applied Cys and ALA as effective compounds in wheat cultivation under water deficit conditions.
format	article
author	Amr Elkelish Mohamed M. El-Mogy Gniewko Niedbała Magdalena Piekutowska Mohamed A. M. Atia Maha M. A. Hamada Mostafa Shahin Soumya Mukherjee Ahmed Abou El-Yazied Mohamed Shebl Mohammad Shah Jahan Ali Osman Hany G. Abd El-Gawad Hatem Ashour Reham Farag Samy Selim Mohamed F. M. Ibrahim
author_facet	Amr Elkelish Mohamed M. El-Mogy Gniewko Niedbała Magdalena Piekutowska Mohamed A. M. Atia Maha M. A. Hamada Mostafa Shahin Soumya Mukherjee Ahmed Abou El-Yazied Mohamed Shebl Mohammad Shah Jahan Ali Osman Hany G. Abd El-Gawad Hatem Ashour Reham Farag Samy Selim Mohamed F. M. Ibrahim
author_sort	Amr Elkelish
title	Roles of Exogenous α-Lipoic Acid and Cysteine in Mitigation of Drought Stress and Restoration of Grain Quality in Wheat
title_short	Roles of Exogenous α-Lipoic Acid and Cysteine in Mitigation of Drought Stress and Restoration of Grain Quality in Wheat
title_full	Roles of Exogenous α-Lipoic Acid and Cysteine in Mitigation of Drought Stress and Restoration of Grain Quality in Wheat
title_fullStr	Roles of Exogenous α-Lipoic Acid and Cysteine in Mitigation of Drought Stress and Restoration of Grain Quality in Wheat
title_full_unstemmed	Roles of Exogenous α-Lipoic Acid and Cysteine in Mitigation of Drought Stress and Restoration of Grain Quality in Wheat
title_sort	roles of exogenous α-lipoic acid and cysteine in mitigation of drought stress and restoration of grain quality in wheat
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/7c3eed0308884de08f93bf13f13e71dd
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Roles of Exogenous α-Lipoic Acid and Cysteine in Mitigation of Drought Stress and Restoration of Grain Quality in Wheat

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