Dicer-2 processes diverse viral RNA species.

RNA silencing pathways play critical roles in gene regulation, virus infection, and transposon control. RNA interference (RNAi) is mediated by small interfering RNAs (siRNAs), which are liberated from double-stranded (ds)RNA precursors by Dicer and guide the RNA-induced silencing complex (RISC) to t...

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Autores principales:	Leah R Sabin, Qi Zheng, Pramod Thekkat, Jamie Yang, Gregory J Hannon, Brian D Gregory, Matthew Tudor, Sara Cherry
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7c3f67634832427fa5782983209ad8d4
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spelling	oai:doaj.org-article:7c3f67634832427fa5782983209ad8d42021-11-18T07:57:57ZDicer-2 processes diverse viral RNA species.1932-620310.1371/journal.pone.0055458https://doaj.org/article/7c3f67634832427fa5782983209ad8d42013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23424633/?tool=EBIhttps://doaj.org/toc/1932-6203RNA silencing pathways play critical roles in gene regulation, virus infection, and transposon control. RNA interference (RNAi) is mediated by small interfering RNAs (siRNAs), which are liberated from double-stranded (ds)RNA precursors by Dicer and guide the RNA-induced silencing complex (RISC) to targets. Although principles governing small RNA sorting into RISC have been uncovered, the spectrum of RNA species that can be targeted by Dicer proteins, particularly the viral RNAs present during an infection, are poorly understood. Dicer-2 potently restricts viral infection in insects by generating virus-derived siRNAs from viral RNA. To better characterize the substrates of Dicer-2, we examined the virus-derived siRNAs produced during the Drosophila antiviral RNAi response to four different viruses using high-throughput sequencing. We found that each virus was uniquely targeted by the RNAi pathway; dicing substrates included dsRNA replication intermediates and intramolecular RNA stem loops. For instance, a putative intergenic RNA hairpin encoded by Rift Valley Fever virus generates abundant small RNAs in both Drosophila and mosquito cells, while repetitive sequences within the genomic termini of Vaccinia virus, which give rise to abundant small RNAs in Drosophila, were found to be transcribed in both insect and mammalian cells. Moreover, we provide evidence that the RNA species targeted by Dicer-2 can be modulated by the presence of a viral suppressor of RNAi. This study uncovered several novel, heavily targeted features within viral genomes, offering insight into viral replication, viral immune evasion strategies, and the mechanism of antiviral RNAi.Leah R SabinQi ZhengPramod ThekkatJamie YangGregory J HannonBrian D GregoryMatthew TudorSara CherryPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 2, p e55458 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Leah R Sabin Qi Zheng Pramod Thekkat Jamie Yang Gregory J Hannon Brian D Gregory Matthew Tudor Sara Cherry Dicer-2 processes diverse viral RNA species.
description	RNA silencing pathways play critical roles in gene regulation, virus infection, and transposon control. RNA interference (RNAi) is mediated by small interfering RNAs (siRNAs), which are liberated from double-stranded (ds)RNA precursors by Dicer and guide the RNA-induced silencing complex (RISC) to targets. Although principles governing small RNA sorting into RISC have been uncovered, the spectrum of RNA species that can be targeted by Dicer proteins, particularly the viral RNAs present during an infection, are poorly understood. Dicer-2 potently restricts viral infection in insects by generating virus-derived siRNAs from viral RNA. To better characterize the substrates of Dicer-2, we examined the virus-derived siRNAs produced during the Drosophila antiviral RNAi response to four different viruses using high-throughput sequencing. We found that each virus was uniquely targeted by the RNAi pathway; dicing substrates included dsRNA replication intermediates and intramolecular RNA stem loops. For instance, a putative intergenic RNA hairpin encoded by Rift Valley Fever virus generates abundant small RNAs in both Drosophila and mosquito cells, while repetitive sequences within the genomic termini of Vaccinia virus, which give rise to abundant small RNAs in Drosophila, were found to be transcribed in both insect and mammalian cells. Moreover, we provide evidence that the RNA species targeted by Dicer-2 can be modulated by the presence of a viral suppressor of RNAi. This study uncovered several novel, heavily targeted features within viral genomes, offering insight into viral replication, viral immune evasion strategies, and the mechanism of antiviral RNAi.
format	article
author	Leah R Sabin Qi Zheng Pramod Thekkat Jamie Yang Gregory J Hannon Brian D Gregory Matthew Tudor Sara Cherry
author_facet	Leah R Sabin Qi Zheng Pramod Thekkat Jamie Yang Gregory J Hannon Brian D Gregory Matthew Tudor Sara Cherry
author_sort	Leah R Sabin
title	Dicer-2 processes diverse viral RNA species.
title_short	Dicer-2 processes diverse viral RNA species.
title_full	Dicer-2 processes diverse viral RNA species.
title_fullStr	Dicer-2 processes diverse viral RNA species.
title_full_unstemmed	Dicer-2 processes diverse viral RNA species.
title_sort	dicer-2 processes diverse viral rna species.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/7c3f67634832427fa5782983209ad8d4
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Dicer-2 processes diverse viral RNA species.

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