Dual effects and mechanism of TiO2 nanotube arrays in reducing bacterial colonization and enhancing C3H10T1/2 cell adhesion

Zhaoxiang Peng,1,2,* Jiahua Ni,3,* Kang Zheng,2 Yandong Shen,2 Xiaoqing Wang,1 Guo He,3 Sungho Jin,4 Tingting Tang1 1Shanghai Key Laboratory of Orthopaedic Implants, Department of Orthopaedic Surgery, Shanghai Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai...

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Autores principales:	Peng Z, Ni J, Zheng K, Shen Y, Wang X, He G, Jin S, Tang T
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2013
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/7c4287196f964f2c9e379d60c139b159
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spelling	oai:doaj.org-article:7c4287196f964f2c9e379d60c139b1592021-12-02T03:00:38ZDual effects and mechanism of TiO2 nanotube arrays in reducing bacterial colonization and enhancing C3H10T1/2 cell adhesion1176-91141178-2013https://doaj.org/article/7c4287196f964f2c9e379d60c139b1592013-08-01T00:00:00Zhttp://www.dovepress.com/dual-effects-and-mechanism-of-tio2-nanotube-arrays-in-reducing-bacteri-a14045https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Zhaoxiang Peng,1,2,* Jiahua Ni,3,* Kang Zheng,2 Yandong Shen,2 Xiaoqing Wang,1 Guo He,3 Sungho Jin,4 Tingting Tang1 1Shanghai Key Laboratory of Orthopaedic Implants, Department of Orthopaedic Surgery, Shanghai Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, People's Republic of China; 2Department of Orthopaedic Surgery, Ningbo Medical Treatment Center Lihuili Hospital, Ningbo, People's Republic of China; 3State Key Lab of Metal Matrix Composites, School of Materials Science and Engineering, Shanghai Jiao Tong University, Shanghai, People's Republic of China; 4Materials Science and Engineering, University of California, San Diego, La Jolla, CA, USA *These authors contributed equally to this work Abstract: Competition occurs between the osteoblasts in regional microenvironments and pathogens introduced during surgery, on the surface of bone implants, such as joint prostheses. The aim of this study was to modulate bacterial and osteoblast adhesion on implant surfaces by using a nanotube array. Titanium oxide (TiO2) nanotube arrays, 30 nm or 80 nm in diameter, were prepared by a two-step anodization on titanium substrates. Mechanically polished and acid-etched titanium samples were also prepared to serve as control groups. The standard strains of Staphylococcus epidermidis (S. epidermidis, American Type Culture Collection [ATCC]35984) and mouse C3H10T1/2 cell lines with osteogenic potential were used to evaluate the different responses to the nanotube arrays, in bacteria and eukaryotic cells. We found that the initial adhesion and colonization of S. epidermidis on the surface of the TiO2 nanotube arrays were significantly reduced and that the adhesion of C3H10T1/2 cells on the surface of the TiO2 nanotube arrays was significantly enhanced when compared with the control samples. Based on a surface analysis of all four groups, we observed increased surface roughness, decreased water contact angles, and an enhanced concentration of oxygen and fluorine atoms on the TiO2 nanotube surface. We conclude that the TiO2 nanotube surface can reduce bacterial colonization and enhance C3H10T1/2 cell adhesion; multiple physical and chemical properties of the TiO2 nanotube surface may contribute to these dual effects. Keywords: bacterial adhesion, titanium implant, surface modificationPeng ZNi JZheng KShen YWang XHe GJin STang TDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2013, Iss default, Pp 3093-3105 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Peng Z Ni J Zheng K Shen Y Wang X He G Jin S Tang T Dual effects and mechanism of TiO2 nanotube arrays in reducing bacterial colonization and enhancing C3H10T1/2 cell adhesion
description	Zhaoxiang Peng,1,2,* Jiahua Ni,3,* Kang Zheng,2 Yandong Shen,2 Xiaoqing Wang,1 Guo He,3 Sungho Jin,4 Tingting Tang1 1Shanghai Key Laboratory of Orthopaedic Implants, Department of Orthopaedic Surgery, Shanghai Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, People's Republic of China; 2Department of Orthopaedic Surgery, Ningbo Medical Treatment Center Lihuili Hospital, Ningbo, People's Republic of China; 3State Key Lab of Metal Matrix Composites, School of Materials Science and Engineering, Shanghai Jiao Tong University, Shanghai, People's Republic of China; 4Materials Science and Engineering, University of California, San Diego, La Jolla, CA, USA *These authors contributed equally to this work Abstract: Competition occurs between the osteoblasts in regional microenvironments and pathogens introduced during surgery, on the surface of bone implants, such as joint prostheses. The aim of this study was to modulate bacterial and osteoblast adhesion on implant surfaces by using a nanotube array. Titanium oxide (TiO2) nanotube arrays, 30 nm or 80 nm in diameter, were prepared by a two-step anodization on titanium substrates. Mechanically polished and acid-etched titanium samples were also prepared to serve as control groups. The standard strains of Staphylococcus epidermidis (S. epidermidis, American Type Culture Collection [ATCC]35984) and mouse C3H10T1/2 cell lines with osteogenic potential were used to evaluate the different responses to the nanotube arrays, in bacteria and eukaryotic cells. We found that the initial adhesion and colonization of S. epidermidis on the surface of the TiO2 nanotube arrays were significantly reduced and that the adhesion of C3H10T1/2 cells on the surface of the TiO2 nanotube arrays was significantly enhanced when compared with the control samples. Based on a surface analysis of all four groups, we observed increased surface roughness, decreased water contact angles, and an enhanced concentration of oxygen and fluorine atoms on the TiO2 nanotube surface. We conclude that the TiO2 nanotube surface can reduce bacterial colonization and enhance C3H10T1/2 cell adhesion; multiple physical and chemical properties of the TiO2 nanotube surface may contribute to these dual effects. Keywords: bacterial adhesion, titanium implant, surface modification
format	article
author	Peng Z Ni J Zheng K Shen Y Wang X He G Jin S Tang T
author_facet	Peng Z Ni J Zheng K Shen Y Wang X He G Jin S Tang T
author_sort	Peng Z
title	Dual effects and mechanism of TiO2 nanotube arrays in reducing bacterial colonization and enhancing C3H10T1/2 cell adhesion
title_short	Dual effects and mechanism of TiO2 nanotube arrays in reducing bacterial colonization and enhancing C3H10T1/2 cell adhesion
title_full	Dual effects and mechanism of TiO2 nanotube arrays in reducing bacterial colonization and enhancing C3H10T1/2 cell adhesion
title_fullStr	Dual effects and mechanism of TiO2 nanotube arrays in reducing bacterial colonization and enhancing C3H10T1/2 cell adhesion
title_full_unstemmed	Dual effects and mechanism of TiO2 nanotube arrays in reducing bacterial colonization and enhancing C3H10T1/2 cell adhesion
title_sort	dual effects and mechanism of tio2 nanotube arrays in reducing bacterial colonization and enhancing c3h10t1/2 cell adhesion
publisher	Dove Medical Press
publishDate	2013
url	https://doaj.org/article/7c4287196f964f2c9e379d60c139b159
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Dual effects and mechanism of TiO2 nanotube arrays in reducing bacterial colonization and enhancing C3H10T1/2 cell adhesion

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