Pyrophosphate-Enhanced Oxidase Activity of Cerium Oxide Nanoparticles for Colorimetric Detection of Nucleic Acids

In recent years, cerium oxide (CeO<sub>2</sub>) nanoparticles (NPs) have drawn significant attention owing to their intrinsic enzyme mimetic properties, which make them powerful tools for biomolecular detection. In this work, we evaluated the effect of pyrophosphate (PPi) on the oxidase...

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Autores principales:	Seokhwan Kim, Jinjoo Han, Heeseok Chung, Yong-Keun Choi, Ayemeh Bagheri Hashkavayi, Yu Zhou, Ki Soo Park
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	cerium oxide colorimetry nucleic acid oxidase activity pyrophosphate Chemical technology TP1-1185
Acceso en línea:	https://doaj.org/article/7c45b5d480ed46edbe6b7eecc338ecfd
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spelling	oai:doaj.org-article:7c45b5d480ed46edbe6b7eecc338ecfd2021-11-25T18:57:30ZPyrophosphate-Enhanced Oxidase Activity of Cerium Oxide Nanoparticles for Colorimetric Detection of Nucleic Acids10.3390/s212275671424-8220https://doaj.org/article/7c45b5d480ed46edbe6b7eecc338ecfd2021-11-01T00:00:00Zhttps://www.mdpi.com/1424-8220/21/22/7567https://doaj.org/toc/1424-8220In recent years, cerium oxide (CeO<sub>2</sub>) nanoparticles (NPs) have drawn significant attention owing to their intrinsic enzyme mimetic properties, which make them powerful tools for biomolecular detection. In this work, we evaluated the effect of pyrophosphate (PPi) on the oxidase activity of CeO<sub>2</sub> NPs. The presence of PPi was found to enhance the oxidase activity of CeO<sub>2</sub> NPs, with enhanced colorimetric signals. This particular effect was then used for the colorimetric detection of target nucleic acids. Overall, the PPi-enhanced colorimetric signals of CeO<sub>2</sub> NPs oxidase activity were suppressed by the presence of the target nucleic acids. Compared with previous studies using CeO<sub>2</sub> NPs only, our proposed system significantly improved the signal change (ca. 200%), leading to more sensitive and reproducible colorimetric analysis of target nucleic acids. As a proof-of-concept study, the proposed system was successfully applied to the highly selective and sensitive detection of polymerase chain reaction products derived from <i>Klebsiella pneumoniae.</i> Our findings will benefit the rapid detection of nucleic acid biomarkers (e.g., pathogenic bacterial DNA or RNA) in point-of-care settings.Seokhwan KimJinjoo HanHeeseok ChungYong-Keun ChoiAyemeh Bagheri HashkavayiYu ZhouKi Soo ParkMDPI AGarticlecerium oxidecolorimetrynucleic acidoxidase activitypyrophosphateChemical technologyTP1-1185ENSensors, Vol 21, Iss 7567, p 7567 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	cerium oxide colorimetry nucleic acid oxidase activity pyrophosphate Chemical technology TP1-1185
spellingShingle	cerium oxide colorimetry nucleic acid oxidase activity pyrophosphate Chemical technology TP1-1185 Seokhwan Kim Jinjoo Han Heeseok Chung Yong-Keun Choi Ayemeh Bagheri Hashkavayi Yu Zhou Ki Soo Park Pyrophosphate-Enhanced Oxidase Activity of Cerium Oxide Nanoparticles for Colorimetric Detection of Nucleic Acids
description	In recent years, cerium oxide (CeO<sub>2</sub>) nanoparticles (NPs) have drawn significant attention owing to their intrinsic enzyme mimetic properties, which make them powerful tools for biomolecular detection. In this work, we evaluated the effect of pyrophosphate (PPi) on the oxidase activity of CeO<sub>2</sub> NPs. The presence of PPi was found to enhance the oxidase activity of CeO<sub>2</sub> NPs, with enhanced colorimetric signals. This particular effect was then used for the colorimetric detection of target nucleic acids. Overall, the PPi-enhanced colorimetric signals of CeO<sub>2</sub> NPs oxidase activity were suppressed by the presence of the target nucleic acids. Compared with previous studies using CeO<sub>2</sub> NPs only, our proposed system significantly improved the signal change (ca. 200%), leading to more sensitive and reproducible colorimetric analysis of target nucleic acids. As a proof-of-concept study, the proposed system was successfully applied to the highly selective and sensitive detection of polymerase chain reaction products derived from <i>Klebsiella pneumoniae.</i> Our findings will benefit the rapid detection of nucleic acid biomarkers (e.g., pathogenic bacterial DNA or RNA) in point-of-care settings.
format	article
author	Seokhwan Kim Jinjoo Han Heeseok Chung Yong-Keun Choi Ayemeh Bagheri Hashkavayi Yu Zhou Ki Soo Park
author_facet	Seokhwan Kim Jinjoo Han Heeseok Chung Yong-Keun Choi Ayemeh Bagheri Hashkavayi Yu Zhou Ki Soo Park
author_sort	Seokhwan Kim
title	Pyrophosphate-Enhanced Oxidase Activity of Cerium Oxide Nanoparticles for Colorimetric Detection of Nucleic Acids
title_short	Pyrophosphate-Enhanced Oxidase Activity of Cerium Oxide Nanoparticles for Colorimetric Detection of Nucleic Acids
title_full	Pyrophosphate-Enhanced Oxidase Activity of Cerium Oxide Nanoparticles for Colorimetric Detection of Nucleic Acids
title_fullStr	Pyrophosphate-Enhanced Oxidase Activity of Cerium Oxide Nanoparticles for Colorimetric Detection of Nucleic Acids
title_full_unstemmed	Pyrophosphate-Enhanced Oxidase Activity of Cerium Oxide Nanoparticles for Colorimetric Detection of Nucleic Acids
title_sort	pyrophosphate-enhanced oxidase activity of cerium oxide nanoparticles for colorimetric detection of nucleic acids
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/7c45b5d480ed46edbe6b7eecc338ecfd
work_keys_str_mv	AT seokhwankim pyrophosphateenhancedoxidaseactivityofceriumoxidenanoparticlesforcolorimetricdetectionofnucleicacids AT jinjoohan pyrophosphateenhancedoxidaseactivityofceriumoxidenanoparticlesforcolorimetricdetectionofnucleicacids AT heeseokchung pyrophosphateenhancedoxidaseactivityofceriumoxidenanoparticlesforcolorimetricdetectionofnucleicacids AT yongkeunchoi pyrophosphateenhancedoxidaseactivityofceriumoxidenanoparticlesforcolorimetricdetectionofnucleicacids AT ayemehbagherihashkavayi pyrophosphateenhancedoxidaseactivityofceriumoxidenanoparticlesforcolorimetricdetectionofnucleicacids AT yuzhou pyrophosphateenhancedoxidaseactivityofceriumoxidenanoparticlesforcolorimetricdetectionofnucleicacids AT kisoopark pyrophosphateenhancedoxidaseactivityofceriumoxidenanoparticlesforcolorimetricdetectionofnucleicacids
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Pyrophosphate-Enhanced Oxidase Activity of Cerium Oxide Nanoparticles for Colorimetric Detection of Nucleic Acids

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