Rapid simultaneous clinical monitoring of five oral anti-coagulant drugs in human urine using green microextraction technique coupled with LC–MS/MS
Objectives: There is no analytical method for simultaneous monitoring of warfarin and novel direct oral anticoagulants (NOACs) in human urine samples in a single run in the clinics. Although several studies have reported their measurements in human blood samples, but its sample collection is more in...
Guardado en:
| Autores principales: | , , , , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Elsevier
2021
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/7c4917bde92c4ec8a008ce4906bb281a |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:7c4917bde92c4ec8a008ce4906bb281a |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:7c4917bde92c4ec8a008ce4906bb281a2021-11-18T04:43:58ZRapid simultaneous clinical monitoring of five oral anti-coagulant drugs in human urine using green microextraction technique coupled with LC–MS/MS1018-364710.1016/j.jksus.2021.101602https://doaj.org/article/7c4917bde92c4ec8a008ce4906bb281a2021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S1018364721002640https://doaj.org/toc/1018-3647Objectives: There is no analytical method for simultaneous monitoring of warfarin and novel direct oral anticoagulants (NOACs) in human urine samples in a single run in the clinics. Although several studies have reported their measurements in human blood samples, but its sample collection is more invasive and time-consuming, thereby challenging to monitor frequently. Methods: In this work, we developed a fast microextraction technique (ultrasound-assisted salt-induced liquid-liquid microextraction, USA-SI-LLME) coupled with high-performance liquid chromatography-tandem mass spectrometry (HPLC–MS/MS) to rapidly quantify four commonly used NOACs drugs (apixaban, dabigatran, edoxaban, and rivaroxaban) and warfarin in human urine samples. USA-SI-LLME conditions were optimized using a water-miscible organic solvent as an extraction solvent, high salt concentrations, sample pH, and extraction time (∼5.5 min). Results and conclusions: The analytical method showed excellent linearities from 0.5 to 500 μg/L for apixaban, edoxaban, rivaroxaban, warfarin, and 1 ∼ 500 μg/L for dabigatran. Intra- and inter-day precision values were <9.31% and R2 > 0.99 for all analytes. Limits of detection ranged between 0.07 ∼ 0.18 μg/L, and relative recoveries ranged between 92.18 and 110.15%. This method was successfully applied to analyze 15 one-spot urine samples from 15 clinical patients who regularly took warfarin or NOACs, and high accuracy was found. We concluded that this method could be used as a non-invasive high-throughput and rapid monitoring of NOACs and warfarin in human urine in clinical settings and could provide timely analysis during emergency care.Tzu-Yu PanWei-Chung TsaiChun-Hsiang TanChing-Mei ChengWei ChenThiagarajan SoundappanMariadhas Valan ArasuNaif Abdullah Al-DhabiChia-Fang WuVinoth Kumar PonnusamyMing-Tsang WuElsevierarticleNovel direct oral anticoagulantsWarfarinMicroextraction techniqueHuman urineLiquid chromatography–tandem mass spectrometryScience (General)Q1-390ENJournal of King Saud University: Science, Vol 33, Iss 8, Pp 101602- (2021) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Novel direct oral anticoagulants Warfarin Microextraction technique Human urine Liquid chromatography–tandem mass spectrometry Science (General) Q1-390 |
| spellingShingle |
Novel direct oral anticoagulants Warfarin Microextraction technique Human urine Liquid chromatography–tandem mass spectrometry Science (General) Q1-390 Tzu-Yu Pan Wei-Chung Tsai Chun-Hsiang Tan Ching-Mei Cheng Wei Chen Thiagarajan Soundappan Mariadhas Valan Arasu Naif Abdullah Al-Dhabi Chia-Fang Wu Vinoth Kumar Ponnusamy Ming-Tsang Wu Rapid simultaneous clinical monitoring of five oral anti-coagulant drugs in human urine using green microextraction technique coupled with LC–MS/MS |
| description |
Objectives: There is no analytical method for simultaneous monitoring of warfarin and novel direct oral anticoagulants (NOACs) in human urine samples in a single run in the clinics. Although several studies have reported their measurements in human blood samples, but its sample collection is more invasive and time-consuming, thereby challenging to monitor frequently. Methods: In this work, we developed a fast microextraction technique (ultrasound-assisted salt-induced liquid-liquid microextraction, USA-SI-LLME) coupled with high-performance liquid chromatography-tandem mass spectrometry (HPLC–MS/MS) to rapidly quantify four commonly used NOACs drugs (apixaban, dabigatran, edoxaban, and rivaroxaban) and warfarin in human urine samples. USA-SI-LLME conditions were optimized using a water-miscible organic solvent as an extraction solvent, high salt concentrations, sample pH, and extraction time (∼5.5 min). Results and conclusions: The analytical method showed excellent linearities from 0.5 to 500 μg/L for apixaban, edoxaban, rivaroxaban, warfarin, and 1 ∼ 500 μg/L for dabigatran. Intra- and inter-day precision values were <9.31% and R2 > 0.99 for all analytes. Limits of detection ranged between 0.07 ∼ 0.18 μg/L, and relative recoveries ranged between 92.18 and 110.15%. This method was successfully applied to analyze 15 one-spot urine samples from 15 clinical patients who regularly took warfarin or NOACs, and high accuracy was found. We concluded that this method could be used as a non-invasive high-throughput and rapid monitoring of NOACs and warfarin in human urine in clinical settings and could provide timely analysis during emergency care. |
| format |
article |
| author |
Tzu-Yu Pan Wei-Chung Tsai Chun-Hsiang Tan Ching-Mei Cheng Wei Chen Thiagarajan Soundappan Mariadhas Valan Arasu Naif Abdullah Al-Dhabi Chia-Fang Wu Vinoth Kumar Ponnusamy Ming-Tsang Wu |
| author_facet |
Tzu-Yu Pan Wei-Chung Tsai Chun-Hsiang Tan Ching-Mei Cheng Wei Chen Thiagarajan Soundappan Mariadhas Valan Arasu Naif Abdullah Al-Dhabi Chia-Fang Wu Vinoth Kumar Ponnusamy Ming-Tsang Wu |
| author_sort |
Tzu-Yu Pan |
| title |
Rapid simultaneous clinical monitoring of five oral anti-coagulant drugs in human urine using green microextraction technique coupled with LC–MS/MS |
| title_short |
Rapid simultaneous clinical monitoring of five oral anti-coagulant drugs in human urine using green microextraction technique coupled with LC–MS/MS |
| title_full |
Rapid simultaneous clinical monitoring of five oral anti-coagulant drugs in human urine using green microextraction technique coupled with LC–MS/MS |
| title_fullStr |
Rapid simultaneous clinical monitoring of five oral anti-coagulant drugs in human urine using green microextraction technique coupled with LC–MS/MS |
| title_full_unstemmed |
Rapid simultaneous clinical monitoring of five oral anti-coagulant drugs in human urine using green microextraction technique coupled with LC–MS/MS |
| title_sort |
rapid simultaneous clinical monitoring of five oral anti-coagulant drugs in human urine using green microextraction technique coupled with lc–ms/ms |
| publisher |
Elsevier |
| publishDate |
2021 |
| url |
https://doaj.org/article/7c4917bde92c4ec8a008ce4906bb281a |
| work_keys_str_mv |
AT tzuyupan rapidsimultaneousclinicalmonitoringoffiveoralanticoagulantdrugsinhumanurineusinggreenmicroextractiontechniquecoupledwithlcmsms AT weichungtsai rapidsimultaneousclinicalmonitoringoffiveoralanticoagulantdrugsinhumanurineusinggreenmicroextractiontechniquecoupledwithlcmsms AT chunhsiangtan rapidsimultaneousclinicalmonitoringoffiveoralanticoagulantdrugsinhumanurineusinggreenmicroextractiontechniquecoupledwithlcmsms AT chingmeicheng rapidsimultaneousclinicalmonitoringoffiveoralanticoagulantdrugsinhumanurineusinggreenmicroextractiontechniquecoupledwithlcmsms AT weichen rapidsimultaneousclinicalmonitoringoffiveoralanticoagulantdrugsinhumanurineusinggreenmicroextractiontechniquecoupledwithlcmsms AT thiagarajansoundappan rapidsimultaneousclinicalmonitoringoffiveoralanticoagulantdrugsinhumanurineusinggreenmicroextractiontechniquecoupledwithlcmsms AT mariadhasvalanarasu rapidsimultaneousclinicalmonitoringoffiveoralanticoagulantdrugsinhumanurineusinggreenmicroextractiontechniquecoupledwithlcmsms AT naifabdullahaldhabi rapidsimultaneousclinicalmonitoringoffiveoralanticoagulantdrugsinhumanurineusinggreenmicroextractiontechniquecoupledwithlcmsms AT chiafangwu rapidsimultaneousclinicalmonitoringoffiveoralanticoagulantdrugsinhumanurineusinggreenmicroextractiontechniquecoupledwithlcmsms AT vinothkumarponnusamy rapidsimultaneousclinicalmonitoringoffiveoralanticoagulantdrugsinhumanurineusinggreenmicroextractiontechniquecoupledwithlcmsms AT mingtsangwu rapidsimultaneousclinicalmonitoringoffiveoralanticoagulantdrugsinhumanurineusinggreenmicroextractiontechniquecoupledwithlcmsms |
| _version_ |
1718425110688301056 |