Herpesviridae prevalence in aqueous humor using PCR

Narumon Keorochana, Wasamon Intaraprasong, Raveewan Choontanom Department of Ophthalmology, Phramongkutklao hospital, Phramongkutklao College of Medicine, Bangkok, Thailand Objective: The aim of this study was to determine the prevalence of Herpesviridae family in aqueous humor and the prevalence...

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Autores principales:	Keorochana N, Intaraprasong W, Choontanom R
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2018
Materias:	Herpesviridae prevalence Aqueous Humor Polymerase Chain Reaction Serology Ophthalmology RE1-994
Acceso en línea:	https://doaj.org/article/7c4c71d44c714a21b025001cc4c2b3ba
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spelling	oai:doaj.org-article:7c4c71d44c714a21b025001cc4c2b3ba2021-12-02T04:24:34ZHerpesviridae prevalence in aqueous humor using PCR1177-5483https://doaj.org/article/7c4c71d44c714a21b025001cc4c2b3ba2018-09-01T00:00:00Zhttps://www.dovepress.com/herpesviridae-prevalence-in-aqueous-humor-using-pcr-peer-reviewed-article-OPTHhttps://doaj.org/toc/1177-5483Narumon Keorochana, Wasamon Intaraprasong, Raveewan Choontanom Department of Ophthalmology, Phramongkutklao hospital, Phramongkutklao College of Medicine, Bangkok, Thailand Objective: The aim of this study was to determine the prevalence of Herpesviridae family in aqueous humor and the prevalence of antibodies against Herpesviridae family in serum.Methods: Participants undergoing cataract surgery were included in the study. Serum for viral serology including herpes simplex virus (HSV), varicella zoster virus (VZV), Epstein–Barr virus (EBV), and cytomegalovirus (CMV) was collected. Aqueous humor specimen was tapped for PCR analysis.Results: Ninety-two participants were included with a mean age of 67.67 years (SD ±12.51). The prevalence of positive serology tests was 83.3% for HSV, 94.0% for VZV, 98.8% for EBV, and 97.6% for CMV. A total of 66 aqueous humor specimens were negative for PCR of Herpesviridae family.Conclusion: This study showed previous HSV, VZV, EBV and CMV infections in >90% of the Thai population, while no viral presence was detected in aqueous humor. Thus, the serology test is unrelated to the presence of virus in the eye. We suggest that PCR is a valuable tool to diagnose intraocular viral infection and detect virus presenting active infection. Keywords: Herpesviridae, prevalence, aqueous humor, PCR, serology, uveitisKeorochana NIntaraprasong WChoontanom RDove Medical PressarticleHerpesviridaeprevalenceAqueous HumorPolymerase Chain ReactionSerologyOphthalmologyRE1-994ENClinical Ophthalmology, Vol Volume 12, Pp 1707-1711 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Herpesviridae prevalence Aqueous Humor Polymerase Chain Reaction Serology Ophthalmology RE1-994
spellingShingle	Herpesviridae prevalence Aqueous Humor Polymerase Chain Reaction Serology Ophthalmology RE1-994 Keorochana N Intaraprasong W Choontanom R Herpesviridae prevalence in aqueous humor using PCR
description	Narumon Keorochana, Wasamon Intaraprasong, Raveewan Choontanom Department of Ophthalmology, Phramongkutklao hospital, Phramongkutklao College of Medicine, Bangkok, Thailand Objective: The aim of this study was to determine the prevalence of Herpesviridae family in aqueous humor and the prevalence of antibodies against Herpesviridae family in serum.Methods: Participants undergoing cataract surgery were included in the study. Serum for viral serology including herpes simplex virus (HSV), varicella zoster virus (VZV), Epstein–Barr virus (EBV), and cytomegalovirus (CMV) was collected. Aqueous humor specimen was tapped for PCR analysis.Results: Ninety-two participants were included with a mean age of 67.67 years (SD ±12.51). The prevalence of positive serology tests was 83.3% for HSV, 94.0% for VZV, 98.8% for EBV, and 97.6% for CMV. A total of 66 aqueous humor specimens were negative for PCR of Herpesviridae family.Conclusion: This study showed previous HSV, VZV, EBV and CMV infections in >90% of the Thai population, while no viral presence was detected in aqueous humor. Thus, the serology test is unrelated to the presence of virus in the eye. We suggest that PCR is a valuable tool to diagnose intraocular viral infection and detect virus presenting active infection. Keywords: Herpesviridae, prevalence, aqueous humor, PCR, serology, uveitis
format	article
author	Keorochana N Intaraprasong W Choontanom R
author_facet	Keorochana N Intaraprasong W Choontanom R
author_sort	Keorochana N
title	Herpesviridae prevalence in aqueous humor using PCR
title_short	Herpesviridae prevalence in aqueous humor using PCR
title_full	Herpesviridae prevalence in aqueous humor using PCR
title_fullStr	Herpesviridae prevalence in aqueous humor using PCR
title_full_unstemmed	Herpesviridae prevalence in aqueous humor using PCR
title_sort	herpesviridae prevalence in aqueous humor using pcr
publisher	Dove Medical Press
publishDate	2018
url	https://doaj.org/article/7c4c71d44c714a21b025001cc4c2b3ba
work_keys_str_mv	AT keorochanan herpesviridaeprevalenceinaqueoushumorusingpcr AT intaraprasongw herpesviridaeprevalenceinaqueoushumorusingpcr AT choontanomr herpesviridaeprevalenceinaqueoushumorusingpcr
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Herpesviridae prevalence in aqueous humor using PCR

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