APPLICATION OF METHOD BASED ON IN VITRO ANTIBODY QUANTIFICATION IN PBMC SUPERNATANT SAMPLES FOR IMMUNOGENICITY EVALUATION OF A (H5N1) AND A (H5N2) POTENTIALLY PANDEMIC INFLUENZA VACCINES IN CLINICAL TRIALS

There are many data worldwide which suggest that the methods for evaluation of influenza vaccines immunogenicity should be improved. The only method validated in Russia is a HAI (haemagglutination inhibition) assay with serum samples from vaccinated volunteers. This assay does not, however, complete...

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Autores principales:	I. V. Losev, S. A. Donina, G. D. Petukhova, M. K. Erofeeva, L. G. Rudenko, A. N. Naykhin
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2016
Materias:	influenza virus immunogenicity immune response humoral mononuclear cells peripheral blood supernates Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/7c57458f56ac4bb19ac66f21f021b387
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spelling	oai:doaj.org-article:7c57458f56ac4bb19ac66f21f021b3872021-11-18T08:03:45ZAPPLICATION OF METHOD BASED ON IN VITRO ANTIBODY QUANTIFICATION IN PBMC SUPERNATANT SAMPLES FOR IMMUNOGENICITY EVALUATION OF A (H5N1) AND A (H5N2) POTENTIALLY PANDEMIC INFLUENZA VACCINES IN CLINICAL TRIALS1563-06252313-741X10.15789/1563-0625-2016-2-129-138https://doaj.org/article/7c57458f56ac4bb19ac66f21f021b3872016-04-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/996https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XThere are many data worldwide which suggest that the methods for evaluation of influenza vaccines immunogenicity should be improved. The only method validated in Russia is a HAI (haemagglutination inhibition) assay with serum samples from vaccinated volunteers. This assay does not, however, completely reflect the vaccine-induced immunological changes. In this study, we evaluated antibody immune responses to A (H5N1) inactivated influenza vaccine boosting in healthy volunteers previously primed with A (H5N2) live attenuated influenza vaccine. We compared three methods of antibody detection: (i) HAI assay with serum samples; (ii) ELISA with serum samples; (iii) ELISA with PBMC (peripheral blood mononuclear cells) culture supernates, i.e., an alternative test based on quantification of antibodies secreted by PBMC in vitro. The latter test was shown to have an advantage over other techniques in IgA and IgG antibody detection at early timepoints (day 7) after vaccination. The first two methods allowed immunogenicity assessment at day 28 after vaccination.Thus, a test based on antibody quantification in PBMC supernatant samples can be used as an alternative method for evaluation of influenza vaccines immunogenicity. This method also exhibits a better strainspecificity.I. V. LosevS. A. DoninaG. D. PetukhovaM. K. ErofeevaL. G. RudenkoA. N. NaykhinSPb RAACIarticleinfluenza virusimmunogenicityimmune responsehumoralmononuclear cellsperipheral bloodsupernatesImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 18, Iss 2, Pp 129-138 (2016)
institution	DOAJ
collection	DOAJ
language	RU
topic	influenza virus immunogenicity immune response humoral mononuclear cells peripheral blood supernates Immunologic diseases. Allergy RC581-607
spellingShingle	influenza virus immunogenicity immune response humoral mononuclear cells peripheral blood supernates Immunologic diseases. Allergy RC581-607 I. V. Losev S. A. Donina G. D. Petukhova M. K. Erofeeva L. G. Rudenko A. N. Naykhin APPLICATION OF METHOD BASED ON IN VITRO ANTIBODY QUANTIFICATION IN PBMC SUPERNATANT SAMPLES FOR IMMUNOGENICITY EVALUATION OF A (H5N1) AND A (H5N2) POTENTIALLY PANDEMIC INFLUENZA VACCINES IN CLINICAL TRIALS
description	There are many data worldwide which suggest that the methods for evaluation of influenza vaccines immunogenicity should be improved. The only method validated in Russia is a HAI (haemagglutination inhibition) assay with serum samples from vaccinated volunteers. This assay does not, however, completely reflect the vaccine-induced immunological changes. In this study, we evaluated antibody immune responses to A (H5N1) inactivated influenza vaccine boosting in healthy volunteers previously primed with A (H5N2) live attenuated influenza vaccine. We compared three methods of antibody detection: (i) HAI assay with serum samples; (ii) ELISA with serum samples; (iii) ELISA with PBMC (peripheral blood mononuclear cells) culture supernates, i.e., an alternative test based on quantification of antibodies secreted by PBMC in vitro. The latter test was shown to have an advantage over other techniques in IgA and IgG antibody detection at early timepoints (day 7) after vaccination. The first two methods allowed immunogenicity assessment at day 28 after vaccination.Thus, a test based on antibody quantification in PBMC supernatant samples can be used as an alternative method for evaluation of influenza vaccines immunogenicity. This method also exhibits a better strainspecificity.
format	article
author	I. V. Losev S. A. Donina G. D. Petukhova M. K. Erofeeva L. G. Rudenko A. N. Naykhin
author_facet	I. V. Losev S. A. Donina G. D. Petukhova M. K. Erofeeva L. G. Rudenko A. N. Naykhin
author_sort	I. V. Losev
title	APPLICATION OF METHOD BASED ON IN VITRO ANTIBODY QUANTIFICATION IN PBMC SUPERNATANT SAMPLES FOR IMMUNOGENICITY EVALUATION OF A (H5N1) AND A (H5N2) POTENTIALLY PANDEMIC INFLUENZA VACCINES IN CLINICAL TRIALS
title_short	APPLICATION OF METHOD BASED ON IN VITRO ANTIBODY QUANTIFICATION IN PBMC SUPERNATANT SAMPLES FOR IMMUNOGENICITY EVALUATION OF A (H5N1) AND A (H5N2) POTENTIALLY PANDEMIC INFLUENZA VACCINES IN CLINICAL TRIALS
title_full	APPLICATION OF METHOD BASED ON IN VITRO ANTIBODY QUANTIFICATION IN PBMC SUPERNATANT SAMPLES FOR IMMUNOGENICITY EVALUATION OF A (H5N1) AND A (H5N2) POTENTIALLY PANDEMIC INFLUENZA VACCINES IN CLINICAL TRIALS
title_fullStr	APPLICATION OF METHOD BASED ON IN VITRO ANTIBODY QUANTIFICATION IN PBMC SUPERNATANT SAMPLES FOR IMMUNOGENICITY EVALUATION OF A (H5N1) AND A (H5N2) POTENTIALLY PANDEMIC INFLUENZA VACCINES IN CLINICAL TRIALS
title_full_unstemmed	APPLICATION OF METHOD BASED ON IN VITRO ANTIBODY QUANTIFICATION IN PBMC SUPERNATANT SAMPLES FOR IMMUNOGENICITY EVALUATION OF A (H5N1) AND A (H5N2) POTENTIALLY PANDEMIC INFLUENZA VACCINES IN CLINICAL TRIALS
title_sort	application of method based on in vitro antibody quantification in pbmc supernatant samples for immunogenicity evaluation of a (h5n1) and a (h5n2) potentially pandemic influenza vaccines in clinical trials
publisher	SPb RAACI
publishDate	2016
url	https://doaj.org/article/7c57458f56ac4bb19ac66f21f021b387
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APPLICATION OF METHOD BASED ON IN VITRO ANTIBODY QUANTIFICATION IN PBMC SUPERNATANT SAMPLES FOR IMMUNOGENICITY EVALUATION OF A (H5N1) AND A (H5N2) POTENTIALLY PANDEMIC INFLUENZA VACCINES IN CLINICAL TRIALS

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