Intracellular Delivery of Proteins into Living Cells by Low-Molecular-Weight Polyethyleneimine

Yueheng Wu,1,2,* Lin jiang,2,* Zixuan Dong,1 Shaoxian Chen,2 Xi-Yong Yu,2 Shunqing Tang1 1National Engineering Research Center for Healthcare Devices, Guangdong Institute of Medical Instruments, Biomedical Engineering Institute, Jinan University, Guangzhou, 510632, People’s Republic of Chi...

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Autores principales:	Wu Y, jiang L, Dong Z, Chen S, Yu XY, Tang S
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2021
Materias:	polyethyleneimine protein delivery nanocomposites low-molecular-weight cytotoxicity Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/7c5971bd488a4263b3c86665afeb9daf
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spelling	oai:doaj.org-article:7c5971bd488a4263b3c86665afeb9daf2021-12-02T15:28:39ZIntracellular Delivery of Proteins into Living Cells by Low-Molecular-Weight Polyethyleneimine1178-2013https://doaj.org/article/7c5971bd488a4263b3c86665afeb9daf2021-06-01T00:00:00Zhttps://www.dovepress.com/intracellular-delivery-of-proteins-into-living-cells-by-low-molecular--peer-reviewed-fulltext-article-IJNhttps://doaj.org/toc/1178-2013Yueheng Wu,1,2,* Lin jiang,2,* Zixuan Dong,1 Shaoxian Chen,2 Xi-Yong Yu,2 Shunqing Tang1 1National Engineering Research Center for Healthcare Devices, Guangdong Institute of Medical Instruments, Biomedical Engineering Institute, Jinan University, Guangzhou, 510632, People’s Republic of China; 2Department of Cardiovascular Surgery, Guangdong Cardiovascular Institute, Guangdong Provincial Key Laboratory of South China Structural Heart Disease, Guangdong Provincial People’s Hospital, Guangdong Academy of Medical Sciences, School of Medicine, South China University of Technology, Guangzhou, 510080, People’s Republic of China*These authors contributed equally to this workCorrespondence: Shunqing TangNational Engineering Research Center for Healthcare Devices, Guangdong Institute of Medical Instruments, Biomedical Engineering Institute, Jinan University, Guangzhou, 510632, People’s Republic of ChinaEmail tshunqt@jnu.edu.cnIntroduction: Intracellular protein delivery is emerging as a potential strategy to revolutionize therapeutics in the field of biomedicine, aiming at treating a wide range of diseases including cancer, inflammatory diseases and other oxidative stress-related disorders with high specificity. However, the current challenges and limitations are addressed to either synthetically or biologically through multipotency of engineering, such as protein modification, insufficient delivery of large-size proteins, deficiency or mutation of proteins, and high cytotoxicity.Methods: We prepared the nanocomposites by mixing protein with PEI1200 at a certain molar ratio and demonstrated that it can deliver proteins into living cells in high efficiency and safety through the following experiments, such as dynamic light scattering, fluorescent detection, agarose gel electrophoresis, ß-Galactosidase activity detection, immunofluorescence staining, digital fluorescent detection, cell viability assay and flow cytometry.Results: The self-assembly of PEI1200/protein nanocomposites with appropriate molar ratio (4:1 and 8:1) could provide efficiently delivery of active proteins to a variety of cell types in the presence of serum. The nanocomposites could continuously release protein up to 96 h in their desired intracellular locations. In addition, these nanocomposites were able to preserve protein activity while maintain low cytotoxicity (when final concentration < 1 μg/mL).Conclusion: Collectively, PEI1200-based delivery system provided an alternative strategy to direct protein delivery in high efficiency and safety, offering increased potential applications in clinical biomedicine.Keywords: polyethyleneimine, protein delivery, nanocomposites, low-molecular-weight, cytotoxicityWu Yjiang LDong ZChen SYu XYTang SDove Medical Pressarticlepolyethyleneimineprotein deliverynanocompositeslow-molecular-weightcytotoxicityMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 16, Pp 4197-4208 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	polyethyleneimine protein delivery nanocomposites low-molecular-weight cytotoxicity Medicine (General) R5-920
spellingShingle	polyethyleneimine protein delivery nanocomposites low-molecular-weight cytotoxicity Medicine (General) R5-920 Wu Y jiang L Dong Z Chen S Yu XY Tang S Intracellular Delivery of Proteins into Living Cells by Low-Molecular-Weight Polyethyleneimine
description	Yueheng Wu,1,2,* Lin jiang,2,* Zixuan Dong,1 Shaoxian Chen,2 Xi-Yong Yu,2 Shunqing Tang1 1National Engineering Research Center for Healthcare Devices, Guangdong Institute of Medical Instruments, Biomedical Engineering Institute, Jinan University, Guangzhou, 510632, People’s Republic of China; 2Department of Cardiovascular Surgery, Guangdong Cardiovascular Institute, Guangdong Provincial Key Laboratory of South China Structural Heart Disease, Guangdong Provincial People’s Hospital, Guangdong Academy of Medical Sciences, School of Medicine, South China University of Technology, Guangzhou, 510080, People’s Republic of China*These authors contributed equally to this workCorrespondence: Shunqing TangNational Engineering Research Center for Healthcare Devices, Guangdong Institute of Medical Instruments, Biomedical Engineering Institute, Jinan University, Guangzhou, 510632, People’s Republic of ChinaEmail tshunqt@jnu.edu.cnIntroduction: Intracellular protein delivery is emerging as a potential strategy to revolutionize therapeutics in the field of biomedicine, aiming at treating a wide range of diseases including cancer, inflammatory diseases and other oxidative stress-related disorders with high specificity. However, the current challenges and limitations are addressed to either synthetically or biologically through multipotency of engineering, such as protein modification, insufficient delivery of large-size proteins, deficiency or mutation of proteins, and high cytotoxicity.Methods: We prepared the nanocomposites by mixing protein with PEI1200 at a certain molar ratio and demonstrated that it can deliver proteins into living cells in high efficiency and safety through the following experiments, such as dynamic light scattering, fluorescent detection, agarose gel electrophoresis, ß-Galactosidase activity detection, immunofluorescence staining, digital fluorescent detection, cell viability assay and flow cytometry.Results: The self-assembly of PEI1200/protein nanocomposites with appropriate molar ratio (4:1 and 8:1) could provide efficiently delivery of active proteins to a variety of cell types in the presence of serum. The nanocomposites could continuously release protein up to 96 h in their desired intracellular locations. In addition, these nanocomposites were able to preserve protein activity while maintain low cytotoxicity (when final concentration < 1 μg/mL).Conclusion: Collectively, PEI1200-based delivery system provided an alternative strategy to direct protein delivery in high efficiency and safety, offering increased potential applications in clinical biomedicine.Keywords: polyethyleneimine, protein delivery, nanocomposites, low-molecular-weight, cytotoxicity
format	article
author	Wu Y jiang L Dong Z Chen S Yu XY Tang S
author_facet	Wu Y jiang L Dong Z Chen S Yu XY Tang S
author_sort	Wu Y
title	Intracellular Delivery of Proteins into Living Cells by Low-Molecular-Weight Polyethyleneimine
title_short	Intracellular Delivery of Proteins into Living Cells by Low-Molecular-Weight Polyethyleneimine
title_full	Intracellular Delivery of Proteins into Living Cells by Low-Molecular-Weight Polyethyleneimine
title_fullStr	Intracellular Delivery of Proteins into Living Cells by Low-Molecular-Weight Polyethyleneimine
title_full_unstemmed	Intracellular Delivery of Proteins into Living Cells by Low-Molecular-Weight Polyethyleneimine
title_sort	intracellular delivery of proteins into living cells by low-molecular-weight polyethyleneimine
publisher	Dove Medical Press
publishDate	2021
url	https://doaj.org/article/7c5971bd488a4263b3c86665afeb9daf
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Intracellular Delivery of Proteins into Living Cells by Low-Molecular-Weight Polyethyleneimine

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